US4087327A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mammalion cell culture process
Assignee: Monsanto Co
•
Filing: 1976-04-12
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Granted: 1978-05-02
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Expires: 1996-04-12
Abstract
Abstract A cell culture reactor system is provided for the growth of cells in vitro which employs elongate hollow or solid fibers arranged in a shallow layer configuration as a matrix for cell attachment in which the flow path of the culture media is directed substantially uniformly through the fiber layer and substantially transverse to the plane of the elongate axes of the fibers.
Inventors
- 👤 Joseph Feder
- 👤 Katharine Ku
- 👤 Mau-Jung Kuo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 02:43
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/04; C12M29/00; C12M29/16
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
76 words
In the method of culturing mammalian cells in vitro whereby the cell growth attaches onto a plurality of elongate fibers disposed in a cell culture reactor having culture medium inlet and outlet means, the improvement comprising employing said fibers in a shallow layer configuration and distributing the culture medium with distributor plate means in a flow path substantially uniformly through said fiber layer and substantially, transverse to the plane of the elongate axes of said fibers.
Claim 2
Dependent Claim
76 words
In the method of culturing mammalian cells in vitro whereby the cell growth attaches onto a plurality of elongate fibers disposed in a cell culture reactor having culture medium inlet and outlet means, the improvement comprising employing said fibers in a shallow layer configuration and distributing the culture medium with distributor plate means in a flow path substantially uniformly through said fiber layer and substantially, transverse to the plane of the elongate axes of said fibers.
Claim 3
Dependent Claim
50 words
The method of claim 1 in which the distributing is carried out by causing the culture medium to flow through an inlet distributor plate having a plurality of small openings therethrough and being positioned substantially parallel to said fiber layer and between said fiber layer and said medium inlet means.
Claim 4
Dependent Claim
14 words
The method of claim 1 in which the distributor plate is a micrometallic filter.
Claim 5
Dependent Claim
22 words
The method of claim 3 in which the micrometallic filter has a pore size of from about 0.5 to about 10 microns.
Claim 6
Dependent Claim
41 words
The method of claim 3 in which an outlet micrometallic filter having a pore size of from about 10 to about 100 microns is positioned substantially parallel to said fiber layer and between said fiber layer and said medium outlet means.
Claim 7
Dependent Claim
20 words
The method of claim 1 in which the fiber layer comprises from one to about 50 thicknesses of individual fibers.
References Cited (Backward Citations)
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US3821087A
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US3883393A
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-05-01 | 1981-10-31 | 1982-05-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-05-01 | 1985-10-31 | 1986-05-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-05-01 | 1989-10-31 | 1990-05-02 | $7700.00 | Window Closed / Lapsed |