Cultivation method of animal cell

Abstract

Abstract PURPOSE:To adjust the pH of a culture fluid very easily without damaging animal cells, etc., by adjusting the concentration of gaseous carbon dioxide in the vapor phase of a cultivation tank in cultivating the animal cells. CONSTITUTION:Animal cells, e.g. various leukemic cells or ascitic cancer cells, are cultivated in a cultivation tank. If the pH of the culture fluid is lower than a given value, air, gaseous carbon dioxide, oxygen gas, nitrogen gas, etc. alone or mixture thereof is introduced into the cultivation tank to reduce the concentration of the gaseous carbon dioxide in the vapor phase of the cultivation tank. If the pH of the culture fluid is higher than the given value, the concentration of the gaseous carbon dioxide in the vapor phase of the cultivation tank is increased.

Inventors

• 👤 Wataru Nakamatsu
• 👤 亘 中松
• 👤 Yoshitoshi Takano
• 👤 高野　佐敏
• 👤 Naoki Nishimura
• 👤 直樹 西村
• 👤 Hiroshiro Shibai
• 👤 柴井　博四郎

Bibliographic Details