Patent US4837170A successfully refreshed from Google Patents.
US4837170A
Expired / Lapsed
US
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Monoclonal antibody, measuring method of glucosylated protein by utilizing the monoclonal antibody, and kit therefor
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1987-01-14
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Granted: 1989-06-06
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Expires: 2007-01-14
Abstract
Abstract For the diagnosis of diabetes, a glucosylated protein in a sample is measured by subjecting the sample to a reducing treatment and then detecting a compound, which contains one or more reduced glucosylated lysine residual groups, in accordance with an immunoassay technique in which the reduced glucosylated lysine residual groups are used as an epitope.
Inventors
- 👤 Yasuo Ohe
- 👤 Makiko Matsuura
- 👤 Fumio Shimizu
- 👤 Yoshito Nakajima
- 👤 Shin Sadahito
- 👤 Kenji Shima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; C; C07; C07K; C07K16/00; C07K16/18; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/811
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
71 words
A method for the measurement of a glucosylated protein in a sample, which comprises subjecting the sample to a reducing treatment and then detecting a protein having one or more reduced glucosylated lysine residual groups with a monoclonal antibody, wherein said monoclonal antibody is one produced by immunization with an antigen selected from the group consisting of reduced glucosylated polylysine, reduced glucosylated human serum albumin, and reduced glucosylated bovine serum albumin.
Claim 2
Independent Claim
71 words
A method for the measurement of a glucosylated protein in a sample, which comprises subjecting the sample to a reducing treatment and then detecting a protein having one or more reduced glucosylated lysine residual groups with a monoclonal antibody, wherein said monoclonal antibody is one produced by immunization with an antigen selected from the group consisting of reduced glucosylated polylysine, reduced glucosylated human serum albumin, and reduced glucosylated bovine serum albumin.
Claim 3
Dependent Claim
34 words
A method as claimed in claim 1, wherein the sample is a protein fraction of a body fluid, said fraction being obtained by an adsorption treatment of the body fluid with a protein-adsorptive carrier.
Claim 4
Dependent Claim
13 words
A method as claimed in claim 1, wherein the body fluid is blood.
Claim 5
Dependent Claim
21 words
A method as claimed in claim 1, wherein the protein-adsorptive carrier is a Coomassie Brilliant Blue G250- or Cibacron Blue-insolubilized carrier.
Claim 6
Dependent Claim
32 words
The method of claim 1, wherein said protein which is detected in said sample is selected from the group consisting of reduced glucosylated human serum albumin and reduced glucosylated bovine serum albumin.
Claim 7
Dependent Claim
39 words
A diagnostic kit for diabetes, which comprises a monoclonal antibody produced by immunization of a subject with an antigen selected from the group consisting of reduced glucosylated polylysine, reduced glucosylated human serum albumin, and reduced glucosylated bovine serum albumin.
Claim 8
Dependent Claim
39 words
A diagnostic kit for diabetes, which comprises a monoclonal antibody produced by immunization of a subject with an antigen selected from the group consisting of reduced glucosylated polylysine, reduced glucosylated human serum albumin, and reduced glucosylated bovine serum albumin.
Claim 9
Dependent Claim
5 words
The hybridoma ATCC HB 9297.
Claim 10
Dependent Claim
5 words
The hybridoma ATCC HB 9297.
Claim 11
Dependent Claim
10 words
A monoclonal antibody produced by the hybridoma ATCC HB 9297.
Claim 12
Dependent Claim
10 words
A monoclonal antibody produced by the hybridoma ATCC HB 9297.
Claim 13
Dependent Claim
11 words
A diagnostic kit containing a monoclonal antibody according to claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-06-05 | 1992-12-05 | 1993-06-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-06-05 | 1996-12-05 | 1997-06-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-06-05 | 2000-12-05 | 2001-06-06 | $7700.00 | Window Closed / Lapsed |