US4474892A
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US
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Two-site immunoassays using monoclonal antibodies of different classes or subclasses and test kits for performing same
Assignee: Leland Stanford Junior University
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Filing: 1983-02-16
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Granted: 1984-10-02
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Expires: 2003-02-16
Abstract
Abstract Two-site immunometric assays for multideterminant antigens are described in which the antigen is reacted with an immobilized monoclonal antibody directed against one antigen determinant and a second monoclonal antibody that is directed against a distinct antigenic determinant and is of a different class or subclass than the immobilized monoclonal antibody. The second monoclonal antibody is labeled in direct versions of the assay and is reacted with a labeled antibody against it in indirect versions of the assay. The immobilizing medium and classes (subclasses) of the antibodies may be selected so as to reduce the likelihood of nonspecific binding enhance sensitivity and/or permit signal amplification.
Inventors
- 👤 Ferid Murad
- 👤 John A. Lewicki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/577; G01N33/543; G01N33/54306; G01N33/573; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/819; Y10S436/824; Y10S436/828
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
79 words
In a two-site immunoassay for detecting the presence of a multideterminant antigen in a sample wherein the antigen in the sample is reacted with (a) an immobilized monoclonal antibody directed aganist one of the antigen determinants; and (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; and (ii) labeled directly or indirectly; the improvement wherein the second monoclonal antibody is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody.
Claim 2
Dependent Claim
79 words
In a two-site immunoassay for detecting the presence of a multideterminant antigen in a sample wherein the antigen in the sample is reacted with (a) an immobilized monoclonal antibody directed aganist one of the antigen determinants; and (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; and (ii) labeled directly or indirectly; the improvement wherein the second monoclonal antibody is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody.
Claim 3
Dependent Claim
46 words
The immunoassay of claim 1 wherein the second monoclonal antibody is of a class or subclass of antibody that may be removed selectively from the immobilizing medium to which the immobilized monoclonal antibody is attached without substantial removal of the immobilized antibody from the immobilizing medium.
Claim 4
Dependent Claim
15 words
The immunoassay of claim 1 wherein the immobilized monoclonal antibody is immobilized by Protein A.
Claim 5
Dependent Claim
83 words
A direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising: (a) incubating the sample with an immobilized first monoclonal antibody against one of the antigen determinants; (b) incubating the incubation product of (a) with a labeled second monoclonal antibody against another distinct determinant of the antigen, the second monoclonal antibody being of a different immunoglobulin class or subclass than the first monoclonal antibody; and (c) detecting the presence of labeled complex in the incubation product of (b).
Claim 6
Dependent Claim
83 words
A direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising: (a) incubating the sample with an immobilized first monoclonal antibody against one of the antigen determinants; (b) incubating the incubation product of (a) with a labeled second monoclonal antibody against another distinct determinant of the antigen, the second monoclonal antibody being of a different immunoglobulin class or subclass than the first monoclonal antibody; and (c) detecting the presence of labeled complex in the incubation product of (b).
Claim 7
Dependent Claim
46 words
The immunoassay of claim 4 wherein the second monoclonal antibody is of a class or subclass of antibody that may be removed selectively from the immobilizing medium to which the immobilized monoclonal antibody is attached without substantial removal of the immobilized antibody from the immobilizing medium.
Claim 8
Dependent Claim
15 words
The immunoassay of claim 6 wherein the immobilized monoclonal antibody is immobilized by Protein A.
Claim 9
Dependent Claim
45 words
The immunoassay of claim 6 wherein after (b) and before (c) the protein A complexes are separated from the incubation mixture of (b) and washed with an aqueous medium having a pH that selectively removes nonspecifically bound second monoclonal antibody from the protein A complexes.
Claim 10
Dependent Claim
98 words
An indirect two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising: (a) incubating the sample with an immobilized first monoclonal antibody against one of the antigen determinants; (b) incubating the incubation product of (a) with a second monoclonal antibody against another distinct determinant of the antigen, the second monoclonal antibody being of a different immunoglobulin class or subclass than the first monoclonal antibody; (c) incubating the incubation product of (b) with labeled antibody against the second monoclonal antibody; and (d) detecting the presence of labeled immobilized complexes in the incubation product of (c).
Claim 11
Dependent Claim
98 words
An indirect two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising: (a) incubating the sample with an immobilized first monoclonal antibody against one of the antigen determinants; (b) incubating the incubation product of (a) with a second monoclonal antibody against another distinct determinant of the antigen, the second monoclonal antibody being of a different immunoglobulin class or subclass than the first monoclonal antibody; (c) incubating the incubation product of (b) with labeled antibody against the second monoclonal antibody; and (d) detecting the presence of labeled immobilized complexes in the incubation product of (c).
Claim 12
Dependent Claim
46 words
The immunoassay of claim 8 wherein the second monoclonal antibody is of a class or subclass of antibody that may be removed selectively from the immobilizing medium to which the immobilized monoclonal antibody is attached without substantial removal of the immobilized antibody from the immobilizing medium.
Claim 13
Dependent Claim
15 words
The immunoassay of claim 8 wherein the immobilized monoclonal antibody is immobilized by Protein A.
Claim 14
Dependent Claim
73 words
A test kit for carrying out a direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) an immobilized monoclonal antibody directed against one of the determinants of the antigen; and (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is labeled; and (iii) is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody.
Claim 15
Dependent Claim
73 words
A test kit for carrying out a direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) an immobilized monoclonal antibody directed against one of the determinants of the antigen; and (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is labeled; and (iii) is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody.
Claim 16
Dependent Claim
47 words
The test kit of claim 11 wherein the second monoclonal antibody is of a class or subclass of antibody that may be removed selectively from the immobilizing medium to which the immobilized monoclonal antibody is attached without substantial removal of the immobilized antibody from the immobilizing medium.
Claim 17
Dependent Claim
16 words
The test kit of claim 12 wherein the immobilized monoclonal antibody is immobilized by Protein A.
Claim 18
Dependent Claim
80 words
A test kit for carrying out a direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) a first monoclonal antibody directed against one of the determinants of the antigen; (b) a first monoclonal antibody immobilizing medium; and (c) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is labeled; and (iii) is of a different immunoglobulin class or subclass than the first monoclonal antibody.
Claim 19
Dependent Claim
80 words
A test kit for carrying out a direct two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) a first monoclonal antibody directed against one of the determinants of the antigen; (b) a first monoclonal antibody immobilizing medium; and (c) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is labeled; and (iii) is of a different immunoglobulin class or subclass than the first monoclonal antibody.
Claim 20
Dependent Claim
77 words
Test kit for carrying out an indirect two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) an immobilized monoclonal antibody directed against one of the determinants of the antigen; (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody; and (c) a labeled antibody against the second monoclonal antibody.
Claim 21
Dependent Claim
77 words
Test kit for carrying out an indirect two-site immunoassay for detecting the presence of a multideterminant antigen in a sample comprising in containers: (a) an immobilized monoclonal antibody directed against one of the determinants of the antigen; (b) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is of a different immunoglobulin class or subclass than the immobilized monoclonal antibody; and (c) a labeled antibody against the second monoclonal antibody.
Claim 22
Dependent Claim
84 words
A test kit for carrying out an indirect two-site immunoassay for detecting the presence of multideterminant antigen in a sample comprising in containers: (a) a first monoclonal antibody directed against one of the determinants of the antigen; (b) a first monoclonal antibody immobilizing medium; (c) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is of a different immunoglobulin class or subclass than the first monoclonal antibody; and (d) a labeled antibody against the second monoclonal antibody.
Claim 23
Dependent Claim
84 words
A test kit for carrying out an indirect two-site immunoassay for detecting the presence of multideterminant antigen in a sample comprising in containers: (a) a first monoclonal antibody directed against one of the determinants of the antigen; (b) a first monoclonal antibody immobilizing medium; (c) a second monoclonal antibody that is (i) directed against another distinct determinant of the antigen; (ii) is of a different immunoglobulin class or subclass than the first monoclonal antibody; and (d) a labeled antibody against the second monoclonal antibody.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-02 | 1988-04-02 | 1988-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-02 | 1992-04-02 | 1992-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-02 | 1996-04-02 | 1996-10-02 | $7700.00 | Window Closed / Lapsed |