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US4808702A
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Decapeptides produced from bioadhesive polyphenolic proteins
Assignee: Enzon Labs Inc
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Filing: 1987-06-01
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Granted: 1989-02-28
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Expires: 2007-06-01
Abstract
Abstract Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group comprising hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently sselected from the group comprising hydrogen and methyl. Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.
Inventors
- 👤 J. Herbert Waite
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:59
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/29
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
30 words
A composition prepared by a method which comprises the steps of: (a) isolating bioadhesive proteins from mussel phenol glands; (b) enzymatically digesting said bioadhesive protein; and (c) recovering said decapeptides.
Claim 2
Dependent Claim
30 words
A composition prepared by a method which comprises the steps of: (a) isolating bioadhesive proteins from mussel phenol glands; (b) enzymatically digesting said bioadhesive protein; and (c) recovering said decapeptides.
Claim 3
Dependent Claim
82 words
A composition prepared by a method which comprises the steps of: (a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR10## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl; (b) digesting said bioadhesive proteins with clostridial collagenase; (c) digesting the resultant collagenase-resistant fragment with trypsin; and (d) recovering said decapeptides.
Claim 4
Dependent Claim
82 words
A composition prepared by a method which comprises the steps of: (a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR10## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl; (b) digesting said bioadhesive proteins with clostridial collagenase; (c) digesting the resultant collagenase-resistant fragment with trypsin; and (d) recovering said decapeptides.
Claim 5
Independent Claim
97 words
A method for the preparation of polyphenolic molecules consisting of from about 1 to about 1000 repeating decapeptide units, which method comprises the step of reacting decapeptides of the formula: ##STR11## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl, with a linking group which is selected from the group comprising amino acid, oligopeptide, and bifunctional spacer, in a neutral or slightly basic buffer, at a temperature, and for a period of time until the reaction is substantially complete.
Claim 6
Independent Claim
97 words
A method for the preparation of polyphenolic molecules consisting of from about 1 to about 1000 repeating decapeptide units, which method comprises the step of reacting decapeptides of the formula: ##STR11## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl, with a linking group which is selected from the group comprising amino acid, oligopeptide, and bifunctional spacer, in a neutral or slightly basic buffer, at a temperature, and for a period of time until the reaction is substantially complete.
Claim 7
Dependent Claim
52 words
Polyphenolic molecules which comprise from about 1 to about 1000 repeating units of the formula: ##STR12## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen and wherein each R is independently selected from the group consisting of hydrogen and methyl, and wherein the repeating units are linked.
Claim 8
Dependent Claim
52 words
Polyphenolic molecules which comprise from about 1 to about 1000 repeating units of the formula: ##STR12## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen and wherein each R is independently selected from the group consisting of hydrogen and methyl, and wherein the repeating units are linked.
References Cited (Backward Citations)
US4585585A
Cited
US4687740A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-28 | 1992-08-29 | 1993-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-28 | 1996-08-29 | 1997-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-28 | 2000-08-29 | 2001-02-28 | $7700.00 | Window Closed / Lapsed |