US4687740A
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Decapeptides produced from bioadhesive polyphenolic proteins
Assignee: University of Connecticut Research and Development Corp
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Filing: 1986-01-21
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Granted: 1987-08-18
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Expires: 2006-01-21
Abstract
Abstract Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group containing hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl. Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.
Inventors
- 👤 J. Herbert Waite
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:47
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
63 words
A method for the preparation of decapeptides of the formula: ##STR10## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating bioadhesive proteins from mussel phenol glands; (b) enzymatically digesting said bioadhesive protein; and (c) recovering said decapeptides.
Claim 2
Independent Claim
63 words
A method for the preparation of decapeptides of the formula: ##STR10## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating bioadhesive proteins from mussel phenol glands; (b) enzymatically digesting said bioadhesive protein; and (c) recovering said decapeptides.
Claim 3
Dependent Claim
18 words
A method, as claimed in claim 1, which enzymatic digestion step comprises digesting said bioadhesive protein with trypsin.
Claim 4
Dependent Claim
29 words
A method, as claimed in claim 1, which enzymatic digestion step comprises: (a) digesting said bioadhesive protein with clostridial collagenase; and (b) digesting the resultant collagenase-resistant fragment with trypsin.
Claim 5
Independent Claim
117 words
A method for the preparation of decapeptides of the formula: ##STR11## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR12## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting said bioadhesive proteins with clostridial collagenase; (c) digesting the resultant collagenase-resistant fragment with trypsin; and (d) recovering said decapeptides.
Claim 6
Independent Claim
117 words
A method for the preparation of decapeptides of the formula: ##STR11## wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl which comprises the steps of: (a) isolating from marine mussels bioadhesive polyphenolic proteins which comprise: ##STR12## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, and wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting said bioadhesive proteins with clostridial collagenase; (c) digesting the resultant collagenase-resistant fragment with trypsin; and (d) recovering said decapeptides.
Claim 7
Independent Claim
108 words
A method for the preparation of decapeptides of the formula: ##STR13## wherein each x is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen amd methyl which comprises the steps of: (a) isolating from marine mussles bioadhesive polyphenolic proteins which comprise: ##STR14## wherein n is a whole number from about 60 to about 100, and wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting the bioadhesive proteins with trypsin; and (c) recovering said decapeptides.
Claim 8
Independent Claim
108 words
A method for the preparation of decapeptides of the formula: ##STR13## wherein each x is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen amd methyl which comprises the steps of: (a) isolating from marine mussles bioadhesive polyphenolic proteins which comprise: ##STR14## wherein n is a whole number from about 60 to about 100, and wherein each X is independently selected from the group consisting of hydroxyl and hydrogen, wherein each R is independently selected from the group consisting of hydrogen and methyl; (b) digesting the bioadhesive proteins with trypsin; and (c) recovering said decapeptides.
Claim 9
Dependent Claim
62 words
A method, as claimed in claim 2, wherein the isolation of said bioadhesive proteins comprises the steps of: a. disecting mussel phenol glands; b. homogenizing said glands in neutral buffer; c. centrifuging the resultant homogenized gland to a pellet; d. homogenizing said pellet in cold-dilute acetic acid; e. centrifuging said homogenized pellet; and f. recovering the bioadhesive protein from the resultant supernatant.
References Cited (Backward Citations)
US4496397A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1990-08-17 | 1991-02-16 | 1991-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-17 | 1995-02-16 | 1995-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-17 | 1999-02-16 | 1999-08-18 | $7700.00 | Window Closed / Lapsed |