US4582805A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilization of biological matter via copolymers of isocyanatoalkyl esters
Assignee: Dow Chemical Co
•
Filing: 1983-12-29
•
Granted: 1986-04-15
•
Expires: 2003-12-29
Abstract
Abstract A process is disclosed for the chemical immobilization of biological material such as bacteria and enzymes containing an active hydrogen with a vinyl addition polymer of an isocyanatoalkyl ester of an ethylenically unsaturated carboxylic acid. The vinyl addition polymer is versatile in that it can be copolymerized with varying amounts and types of comonomers.
Inventors
- 👤 John W. Bozzelli
- 👤 Roberta C. Cheng
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:22
CPC Classifications
C; C08; C08G; C08G18/00; C08G18/06; C08G18/70; C08G18/72; C08G18/728; C08G18/28; C08G18/40; C08G18/64; C08G18/6415; C08G18/6446; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
77 words
A process for chemically immobilizing biological material containing an active hydrogen moiety comprising: (a) contacting said biological material with a vinyl addition polymer comprising a biological immobilizing amount of a polymerized isocyanatoalkyl ester of an ethylenically unsaturated carboxylic acid whereby said vinyl addition polymer has pendent isocyanate groups, and (b) reacting said isocyanate groups pendent from the backbone of said vinyl addition polymer with said active hydrogen moieties of said biological material to immobilize said biological material.
Claim 2
Dependent Claim
77 words
A process for chemically immobilizing biological material containing an active hydrogen moiety comprising: (a) contacting said biological material with a vinyl addition polymer comprising a biological immobilizing amount of a polymerized isocyanatoalkyl ester of an ethylenically unsaturated carboxylic acid whereby said vinyl addition polymer has pendent isocyanate groups, and (b) reacting said isocyanate groups pendent from the backbone of said vinyl addition polymer with said active hydrogen moieties of said biological material to immobilize said biological material.
Claim 3
Dependent Claim
35 words
The process of claim 1 wherein said vinyl addition polymer is a copolymer containing a monomer such that said copolymer is more hydrophilic than 40/60 weight percent copolymer of an isocyanate monomer and methyl methacrylate.
Claim 4
Dependent Claim
12 words
The process of claim 1 wherein said biological material is an enzyme.
Claim 5
Dependent Claim
13 words
The process of claim 1 wherein said biological material is a bacterial cell.
Claim 6
Dependent Claim
15 words
The bacterial cell of claim 4 which is Ampullariella species 3876, 3877, 3965 or 3966.
Claim 7
Dependent Claim
9 words
The Ampullariella species of claim 5 which is 3876.
Claim 8
Dependent Claim
12 words
The process of claim 1 wherein said isocyanatoalkyl ester is 2-isocyanatoethyl methacrylate.
Claim 9
Dependent Claim
18 words
The process of claim 1 wherein said vinyl addition polymer is a copolymer comprising 2-isocyanatoethyl methacrylate and N,N'-dimethylacrylamide.
Claim 10
Dependent Claim
9 words
A product formed from the process of claim 1.
Claim 11
Dependent Claim
36 words
The product of claim 9 wherein said vinyl addition polymer is a copolymer containing a monomer such that said copolymer is more hydrophilic than a 40/60 weight percent copolymer of an isocyanate monomer and methyl methacrylate.
Claim 12
Dependent Claim
12 words
The product of claim 9 wherein said biological material is an enzyme.
Claim 13
Dependent Claim
13 words
The product of claim 9 wherein said biological material is a bacterial cell.
Claim 14
Dependent Claim
15 words
The bacterial cell of claim 12 which is Ampullariella species 3876, 3877, 3965 or 3966.
Claim 15
Dependent Claim
9 words
The Ampullariella species of claim 13 which is 3876.
Claim 16
Dependent Claim
12 words
The product of claim 9 wherein said isocyanatoalkyl ester is 2-isocyanatoethyl methacrylate.
Claim 17
Dependent Claim
18 words
The product of claim 9 wherein said vinyl addition polymer is a copolymer comprising 2-isocyanatoethyl methacrylate and N,N'-dimethylacrylamide.
References Cited (Backward Citations)
CA874457A
Cited
US3672955A
Cited
US3767790A
Cited
US4312946A
Cited
US4195129A
Cited
US4177038A
Cited
US4308349A
Cited
US4440903A
Cited
US4504628A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-14 | 1989-10-14 | 1990-04-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-14 | 1993-10-14 | 1994-04-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-14 | 1997-10-14 | 1998-04-15 | $7700.00 | Window Closed / Lapsed |