US4195129A
Expired / Lapsed
US
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Method for immobilizing enzymes and microbial cells
Assignee: Kansai Paint Co Ltd
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Filing: 1977-12-29
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Granted: 1980-03-25
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Expires: 1997-12-29
Abstract
Abstract A method for immobilizing enzymes or microbial cells which comprises the steps of uniformly mixing an aqueous dispersion of enzymes or microbial cells with a photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photopolymerizable ethylenically unsaturated groups per molecule and hydrophilic groups, and irradiating actinic rays to the above mixture of photo-curable resin and enzymes or microbial cells. The immobilized product is advantageously used in various enzymatic reactions with stable enzyme activity.
Inventors
- 👤 Saburo Fukui
- 👤 Tsutomu Yamamoto
- 👤 Takamitsu Iida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:06
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; C12N11/08; C12N11/082; C12N11/087; C12N11/089; C12N11/093; C12N11/096; C12Q; C12Q1/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
79 words
A method for immobilizing enzymes or microbial cells which is characterized in the steps of: uniformly mixing aqueous dispersion of enzymes or microbial cells with a monomer-free photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photo-polymerizable ethylenically unsaturated groups per molecule and nonionic hydrophilic groups; and irradiating actinic rays having a wave length of 2500 to 6000 A to said mixture, whereby said enzymes or microbial cells are entrapped in said resin.
Claim 2
Independent Claim
79 words
A method for immobilizing enzymes or microbial cells which is characterized in the steps of: uniformly mixing aqueous dispersion of enzymes or microbial cells with a monomer-free photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photo-polymerizable ethylenically unsaturated groups per molecule and nonionic hydrophilic groups; and irradiating actinic rays having a wave length of 2500 to 6000 A to said mixture, whereby said enzymes or microbial cells are entrapped in said resin.
Claim 3
Dependent Claim
71 words
A method for immobilizing enzymes or microbial cells as claimed in claim 1 wherein said mixture additionally contains another monomer-free photo-curable resin having a number average molecular weight of 800 to 100,000, two or more photo-polymerizable ethylenically unsaturated groups per molecule and ionic hydrophilic groups, the ratio of the resin having ionic hydrophilic groups to the resin having nonionic hydrophilic groups being in the range of 1/99 to 50/50 by weight.
Claim 4
Dependent Claim
59 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein said enzymes are urease, glucose oxidase, catalase, glucoamylase, glucose isomerase, invertase, acetoacetic decarboxylase, glucose oxidase-catalase, peroxidase, lactase, D-amino acid oxidase, α-galactosidase, aminoacylase, aspartase or penicillin amidase, and said microbial cells are the cells of Lactobacillus bulgaricus, Aerobacter aerogenes, Bacillus subtilis, Azotobacter vinelandii or Proteus vulgaris.
Claim 5
Dependent Claim
58 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein said photo-curable resin having ionic hydrophilic groups is at least one member selected from the group consisting of salts of high acid value unsaturated polyesters, high acid value unsaturated epoxides, anionic unsaturated acrylic resins, cationic unsaturated acrylic resins, unsaturated polyamines and unsaturated carboxylated cellulose.
Claim 6
Dependent Claim
66 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein said photo-curable resin having nonionic hydrophilic groups is at least one member selected from the group consisting of: polyesters made from polyethylene glycol and acrylic or methacrylic acid, urethanated adduct of the product made from polyisocyanate and polyethylene glycol with 2-hydroxyethyl acrylate or methacrylate, unsaturated cellulose, unsaturated polyvinyl alcohol and unsaturated polyamide.
Claim 7
Dependent Claim
26 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein said ratio is in the range of 3/97 to 20/80 by weight.
Claim 8
Dependent Claim
24 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein a photosensitizer is added to said mixture of photo-curable resins.
Claim 9
Dependent Claim
46 words
A method for immobilizing enzymes or microbial cells as claimed in claim 7, wherein said photosensitizer is at least one member selected from the group consisting of α-carbonyl alcohol, acyloin ether, α-substituted acyloin, naphtol, hydroxyanthracene, axoamide, uranyl nitrate, ferric chloride, mercaptan, disulfide, ascorbic acid and rivoflavin.
Claim 10
Dependent Claim
23 words
A method for immobilizing enzymes or microbial cells as claimed in claim 2, wherein said number average molecular weight is 1,000 to 70,000.
Claim 11
Dependent Claim
22 words
A method for immobilizing enzymes or microbial cells as claimed in claim 4, wherein said high acid value is 40 to 200.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-25 | 1983-09-24 | 1984-03-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-25 | 1987-09-24 | 1988-03-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-25 | 1991-09-24 | 1992-03-25 | $7700.00 | Window Closed / Lapsed |