US4308349A
Expired / Lapsed
US
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Isomerization of glucose to fructose using glucose isomerase from ampullariella
Assignee: Dow Chemical Co
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Filing: 1978-03-09
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Granted: 1981-12-29
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Expires: 1998-03-09
Abstract
Abstract The production of a heat stable glucose isomerase from a microorganism belonging to the genus Ampullariella and a method for using the isomerase to convert glucose to fructose.
Inventors
- 👤 Sharonkay E. Foley
- 👤 Patrick J. Oriel
- 👤 Carol C. Epstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:13
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12N9/00; C12N9/90; C12N9/92; C12P; C12P19/00; C12P19/24; C12R; C12R2001/00; C12R2001/01; C12R2001/045; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
29 words
A process for producing glucose isomerase from a microorganism belonging to the genus Ampullariella which comprises growing the Ampullariella in a culture medium and recovering the glucose isomerase therefrom.
Claim 2
Dependent Claim
29 words
A process for producing glucose isomerase from a microorganism belonging to the genus Ampullariella which comprises growing the Ampullariella in a culture medium and recovering the glucose isomerase therefrom.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the culture medium contains xylose or a xylose supplying source.
Claim 4
Dependent Claim
15 words
The process of claim 1 wherein the microorganism is Ampullariella digitata or a mutant thereof.
Claim 5
Dependent Claim
16 words
The process of claim 1 wherein the microorganism is Ampullariella species 3876 or a mutant thereof.
Claim 6
Dependent Claim
16 words
The process of claim 1 wherein the microorganism is Ampullariella species 3877 or a mutant thereof.
Claim 7
Dependent Claim
16 words
The process of claim 1 wherein the microorganism is Ampullariella species 3965 or a mutant thereof.
Claim 8
Dependent Claim
16 words
The process of claim 1 wherein the microorganism is Ampullariella species 3966 or a mutant thereof.
Claim 9
Dependent Claim
15 words
The process of claim 1 wherein the microorganism is Ampullariella lobata or a mutant thereof.
Claim 10
Dependent Claim
15 words
The process of claim 1 wherein the microorganism is Ampullariella campanulata or a mutant thereof.
Claim 11
Dependent Claim
15 words
The process of claim 1 wherein the microorganism is Ampullariella regularis or a mutant thereof.
Claim 12
Dependent Claim
39 words
A process for isomerizing D-glucose to D-fructose including the step of treating the D-glucose in an aqueous medium with an isomerase produced by a microorganism belonging to the genus Ampullariella thereby converting a portion of the glucose to fructose.
Claim 13
Dependent Claim
39 words
A process for isomerizing D-glucose to D-fructose including the step of treating the D-glucose in an aqueous medium with an isomerase produced by a microorganism belonging to the genus Ampullariella thereby converting a portion of the glucose to fructose.
Claim 14
Dependent Claim
15 words
The process of claim 11 wherein a thermal stabilizing agent is present during the isomerization.
Claim 15
Dependent Claim
24 words
The process of claim 12 wherein the thermal stabilizing agent is a cation selected from the group consisting of cobalt, magnesium, and manganese ions.
Claim 16
Dependent Claim
15 words
The process of claim 13 wherein the glucose isomerase is immobilized on a water-insoluble support.
Claim 17
Dependent Claim
31 words
The process of claim 14 wherein about one pound of immobilized glucose isomerase is present for the isomerization of from about 50 to 5000 pounds of glucose/fructose mixture as dry solids.
Claim 18
Dependent Claim
27 words
The process of claim 15 wherein the pH is from about 6 to about 8.5 and the temperature is from about 45° C. to about 85° C.
Claim 19
Dependent Claim
28 words
The process of claim 11, wherein fructose comprises between about 40 percent and 50 percent of the hexose present in the aqueous medium following treatment with glucose isomerase.
Claim 20
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3876 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31351, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 21
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3876 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31351, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 22
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3877 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31352, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 23
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3877 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31352, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 24
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3965 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31353, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 25
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3965 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31353, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 26
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3966 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31354, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 27
Dependent Claim
46 words
A biologically pure culture of the microorganism Ampullariella species 3966 demonstrating glucose isomerase activity having the identifying characteristics of ATCC 31354, said culture producing glucose isomerase in a recoverable quantity upon fermentation in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and essential minerals.
Claim 28
Dependent Claim
63 words
A culture medium consisting essentially of a biologically pure microorganism selected from the group consisting of Ampullariella species 3876, Ampullariella species 3877, Ampullariella species 3965 and Ampullariella species 3966 or a mutant thereof and an aqueous nutrient culture medium containing assimilable sources of carbon, nitrogen, and essential minerals, said culture medium wherein glucose isomerase is produced in a recoverable quantity upon aerobic fermentation.
Claim 29
Dependent Claim
63 words
A culture medium consisting essentially of a biologically pure microorganism selected from the group consisting of Ampullariella species 3876, Ampullariella species 3877, Ampullariella species 3965 and Ampullariella species 3966 or a mutant thereof and an aqueous nutrient culture medium containing assimilable sources of carbon, nitrogen, and essential minerals, said culture medium wherein glucose isomerase is produced in a recoverable quantity upon aerobic fermentation.
Claim 30
Dependent Claim
19 words
The culture medium of claim 22 further including from about 0.1 percent to 10 percent by weight of xylose.
Claim 31
Dependent Claim
17 words
The culture medium of claim 23 wherein the microorganism is Ampullariella species 3876 or a mutant thereof.
Claim 32
Dependent Claim
17 words
The culture medium of claim 23 wherein the microorganism is Ampullariella species 3877 or a mutant thereof.
Claim 33
Dependent Claim
17 words
The culture medium of claim 23 wherein the microorganism is Ampullariella species 3965 or a mutant thereof.
Claim 34
Dependent Claim
17 words
The culture medium of claim 23 wherein the microorganism is Ampullariella species 3966 or a mutant thereof.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-28 | 1985-06-29 | 1985-12-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-28 | 1989-06-29 | 1989-12-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-28 | 1993-06-29 | 1993-12-29 | $7700.00 | Window Closed / Lapsed |