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Element for electrophoresis

Assignee: DIRECTOR OF FINANCE DIVISION MINISTER'S SECRETARIAT SCIENCE AND TECHNOLOGY AGENCY NO 2-2-1 KASUMIGASEKI CHIYODA-KU TOKYO JAPAN
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Filing: 1984-03-12
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Granted: 1985-10-22
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Expires: 2004-03-12
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Abstract

Abstract An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one specifically selected repeating unit; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, and a compound containing at least one carbamoyl group (modifier).

Inventors

  • 👤 Masashi Ogawa
  • 👤 Hisashi Shiraishi
  • 👤 Teppei Ikeda

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 10:33
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31507; Y10T428/31551; Y10T428/31623; Y10T428/31725; Y10T428/31728; Y10T428/31739; Y10T428/31743; Y10T428/31746; Y10T428/3175; Y10T428/31754; Y10T428/31779; Y10T428/31783; Y10T428/31786; Y10T428/31797; Y10T428/31971

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 503 words
An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of: (1) a repeating unit having the formula (1): ##STR18## in which R 11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; Q 1 is --COO--, --CON(R 11 )--, or an arylene group containing 6-10 carbon atoms; L 1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R 11 )-- and containing 3-15 carbon atoms, or a divalent atom containing at least one linkage selected from the group consisting of --O--, --N(R 11 )--, --CO--, --SO--, --SO 2 --, --SO 3 --, --SO 2 N(R 11 )--, --N(R 11 )CON(R 11 ) and --N(R 11 )COO--, and containing 1-12 carbon atoms, in which R 11 has the same meaning as defined above; R 12 is --CH═CH 2 or --CH 2 CH 2 X 1 , in which X 1 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 1 by a base; and x 1 and y 1 both representing molar percents range from 0 to 99 and from 1 to 100, respectively, and x 1 +y 1 is not less than 90; (2) a repeating unit having the formula (2): ##STR19## in which R 21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; R 22 is --CH═CH 2 or --CH 2 CH 2 X 2 , in which X 2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 2 by a base; L 2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ 2 --, and --COZ 2 R 23 --, in which R 23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z 2 is the oxygen atom or NH; and x 2 and y 2 both representing molar percents range from 10 to 95 and from 5 to 90, respectively, and x 2 +y 2 is not less than 90; and (3) a repeating unit having the formula (3): ##STR20## in which R 31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; L 3 is a divalent linkage group containing 1-20 carbon atoms; X 3 is an active ester; x 3 and y 3 both representing molar percent range from 0 to 95 and from 5 to 100, respectively, and x 3 +y 3 is not less than 90; and m is 0 or 1; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a cross-linking agent in the presence of water, and a compound containing at least one carbamoyl group serving as modifier.
Claim 2
Dependent Claim 503 words
An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of: (1) a repeating unit having the formula (1): ##STR18## in which R 11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; Q 1 is --COO--, --CON(R 11 )--, or an arylene group containing 6-10 carbon atoms; L 1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R 11 )-- and containing 3-15 carbon atoms, or a divalent atom containing at least one linkage selected from the group consisting of --O--, --N(R 11 )--, --CO--, --SO--, --SO 2 --, --SO 3 --, --SO 2 N(R 11 )--, --N(R 11 )CON(R 11 ) and --N(R 11 )COO--, and containing 1-12 carbon atoms, in which R 11 has the same meaning as defined above; R 12 is --CH═CH 2 or --CH 2 CH 2 X 1 , in which X 1 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 1 by a base; and x 1 and y 1 both representing molar percents range from 0 to 99 and from 1 to 100, respectively, and x 1 +y 1 is not less than 90; (2) a repeating unit having the formula (2): ##STR19## in which R 21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; R 22 is --CH═CH 2 or --CH 2 CH 2 X 2 , in which X 2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 2 by a base; L 2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ 2 --, and --COZ 2 R 23 --, in which R 23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z 2 is the oxygen atom or NH; and x 2 and y 2 both representing molar percents range from 10 to 95 and from 5 to 90, respectively, and x 2 +y 2 is not less than 90; and (3) a repeating unit having the formula (3): ##STR20## in which R 31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; L 3 is a divalent linkage group containing 1-20 carbon atoms; X 3 is an active ester; x 3 and y 3 both representing molar percent range from 0 to 95 and from 5 to 100, respectively, and x 3 +y 3 is not less than 90; and m is 0 or 1; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a cross-linking agent in the presence of water, and a compound containing at least one carbamoyl group serving as modifier.
Claim 3
Dependent Claim 22 words
The element for electrophoresis as claimed in claim 1, in which said medium layer for electrophoresis contains a water-soluble polymer and agarose.
Claim 4
Dependent Claim 22 words
The element for electrophoresis as claimed in claim 1 or 2, in which said compound serving as modifier is urea or formamide.
Claim 5
Dependent Claim 23 words
The element for electrophoresis as claimed in claim 1 or 2, in which said support layer is made of a plastic material sheet.
Claim 6
Dependent Claim 20 words
The element for electrophoresis as claimed in claim 4, in which the plastic material sheet is a polyethylene terephthalate sheet.
Claim 7
Dependent Claim 23 words
The element for electrophoresis as claimed in claim 1, in which the adhesive layer consists essentially of a polymer having the formula (1).

References Cited (Backward Citations)

US4012560A Cited
US4048377A Cited
US4383376A Cited
US4415428A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1988-10-21 1989-04-22 1989-10-22 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1992-10-21 1993-04-22 1993-10-22 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1996-10-21 1997-04-22 1997-10-22 $7700.00 Window Closed / Lapsed

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