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US4415428A Expired / Lapsed US ⏱️ 0.0 years remaining

Support for electrophoresis and method of producing same

Assignee: FMC Corp
•
Filing: 1982-01-27
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Granted: 1983-11-15
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Expires: 2002-01-27
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Abstract

Abstract An electrophoretic support comprising a base plate having on at least one side thereof, a layer of an adherent resin containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide. The support provides excellent adhesion for polyacrylamide gels and other electrophoretic media such as agarose.

Inventors

  • 👤 Samuel Nochumson
  • 👤 Henry J. Witt

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 16:31
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747

Claims Analysis

Total Claims: 18
Claim 1
Independent Claim 43 words
A support for an electrophoretic medium comprising a base plate of an organoplastic sheet film and an adherent resin coating applied to at least one side of said base plate, said resin coating containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide.
Claim 2
Dependent Claim 43 words
A support for an electrophoretic medium comprising a base plate of an organoplastic sheet film and an adherent resin coating applied to at least one side of said base plate, said resin coating containing ethylenically unsaturated groups capable of undergoing copolymerization with acrylamide.
Claim 3
Dependent Claim 39 words
The support according to claim 1 wherein the organo plastic sheet film is selected from the class consisting essentially of polystyrene, polypropylene, styrene-acrylonitrile copolymer, polycarbonate, cellulose acetate propionate, cellulose acetate, butyrate, nitrile-acrylonitrile-styrene terpolymer, polyacrylate, polymethacrylate, polyterephthalate and acrylonitrile-butadiene-styrene copolymer.
Claim 4
Dependent Claim 46 words
The support of claim 1 wherein the ethylenically unsaturated groups in the resin consist of ethylenically unsaturated hydrocarbon radicals of 2 to 12 carbon atoms connected to said resin through functional linkages selected from the class consisting of --O--, --S--, --N--, --O(CO)--, S(CO)-- and --(CO)N-- linkages.
Claim 5
Dependent Claim 21 words
The support according to claim 1 wherein the resin coating is in the form of a separately applied film-forming adherent coating.
Claim 6
Dependent Claim 20 words
The support according to claim 4 wherein the coated side of the base plate and the resin coating are hydrophilic.
Claim 7
Dependent Claim 28 words
The support according to claim 5 wherein the hydrophilic surface on the coated side of the organoplastic sheet film is formed by contacting it with an electric discharge.
Claim 8
Dependent Claim 12 words
The support according to claim 3 wherein the resin is a polysaccharide.
Claim 9
Dependent Claim 11 words
The support according to claim 7 wherein the polysaccharide is agarose.
Claim 10
Dependent Claim 37 words
An electrophoretic support comprising a base plate of an organoplastic film sheet one side of which has been rendered hydrophilic by contact with a plasma chamber and having applied to said hydrophilic side a coating of allylglycidylagarose.
Claim 11
Dependent Claim 37 words
An electrophoretic support comprising a base plate of an organoplastic film sheet one side of which has been rendered hydrophilic by contact with a plasma chamber and having applied to said hydrophilic side a coating of allylglycidylagarose.
Claim 12
Dependent Claim 13 words
The support according to claim 9 wherein the organoplastic film is polyester film.
Claim 13
Dependent Claim 18 words
An electrophoretic element, for use in electrophoretic separations, comprising the support of claim 1 coated with electrophoretic medium.
Claim 14
Dependent Claim 22 words
The electrophoretic element of claim 11 wherein the electrophoretic medium is selected from the class consisting of polyacrylamide and agarose gel films.
Claim 15
Dependent Claim 14 words
An electrophoretic element, comprising the support of claim 9 coated with an electrophoretic medium.
Claim 16
Dependent Claim 22 words
The electrophoretic element of claim 13 wherein the electrophoretic medium is selected from the class consisting of polyacrylamide and agarose gel films.
Claim 17
Dependent Claim 15 words
The electrophoretic element of claim 14 wherein the electrophoretic medium is a polyacrylamide gel film.
Claim 18
Dependent Claim 15 words
The electrophoretic element of claim 14 wherein the electrophoretic medium is an agarose gel film.

References Cited (Backward Citations)

US4319975A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1986-11-14 1987-05-16 1987-11-15 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1990-11-14 1991-05-16 1991-11-15 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1994-11-14 1995-05-16 1995-11-15 $7700.00 Window Closed / Lapsed

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