US4293654A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture microcarriers
Assignee: Massachusetts Institute of Technology
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Filing: 1979-07-02
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Granted: 1981-10-06
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Expires: 1999-07-02
Abstract
Abstract Improved cell culture microcarriers, and methods for their production and use, are disclosed herein. These improved microcarriers have positive charge capacities adjusted and/or controlled within a range suitable for good cell growth. One method for producing such improved microcarriers is by treating beads formed from polymers containing pendant hydroxy groups, such as dextran beads, with an aqueous solution of an alkaline material and a chloro- or bromo-substituted tertiary amine under precisely controlled conditions to produce the desired exchange capacity. The resultant positively charged microcarriers have been used in microcarrier cultures to produce outstanding growth of anchorage-dependent cells. Such cells can be harvested, or used for the production of viruses, vaccines, hormones, interferon or other cellular growth by-products.
Inventors
- 👤 David W. Levine
- 👤 William G. Thilly
- 👤 Daniel I. C. Wang
- 👤 Jason S. Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:44
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C07K14/565; A; A61; A61K; A61K38/00; C12N2533/00; C12N2533/70
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
31 words
Cell culture microcarriers having a degree of substitution thereon with positively-charged chemical moieties sufficient to provide a charge capacity of from about 0.1 to about 4.5 meq/gram of dry, untreated microcarriers.
Claim 2
Dependent Claim
31 words
Cell culture microcarriers having a degree of substitution thereon with positively-charged chemical moieties sufficient to provide a charge capacity of from about 0.1 to about 4.5 meq/gram of dry, untreated microcarriers.
Claim 3
Dependent Claim
34 words
Cell culture microcarriers comprising crosslinked dextran beads having a sufficient amount of positively charged groups thereon to provide a charge capacity of between about 0.1 and about 4.5 meq/gram of dry, crosslinked dextran beads.
Claim 4
Dependent Claim
34 words
Cell culture microcarriers comprising crosslinked dextran beads having a sufficient amount of positively charged groups thereon to provide a charge capacity of between about 0.1 and about 4.5 meq/gram of dry, crosslinked dextran beads.
Claim 5
Dependent Claim
14 words
Cell culture microcarriers of claim 2 wherein said positively charged groups comprise diethylaminoethyl groups.
Claim 6
Dependent Claim
56 words
Cell culture microcarriers comprising a reaction product of crosslinked dextran beads and an aqueous solution of a tertiary or quaternary amine and a base, said aqueous solution having an amount and ratio of amine and base sufficient to provide said microcarriers with an exchange capacity of from about 0.1 to about 4.5 meq/gram of dry dextran.
Claim 7
Dependent Claim
56 words
Cell culture microcarriers comprising a reaction product of crosslinked dextran beads and an aqueous solution of a tertiary or quaternary amine and a base, said aqueous solution having an amount and ratio of amine and base sufficient to provide said microcarriers with an exchange capacity of from about 0.1 to about 4.5 meq/gram of dry dextran.
Claim 8
Dependent Claim
11 words
Cell culture microcarriers of claim 4 wherein said amine comprises diethylaminoethyl.
Claim 9
Dependent Claim
12 words
Cell culture microcarriers of claim 5 wherein said base comprises sodium hydroxide.
Claim 10
Independent Claim
54 words
A method of producing cell culture microcarriers comprising soaking crosslinked dextran beads in an aqueous solution of a tertiary or quaternary amine and a base until said beads are substituted with a sufficient amount of amine moieties to produce an exchange capacity thereon of from about 0.1 to about 4.5 meq/gram of dry dextran.
Claim 11
Independent Claim
54 words
A method of producing cell culture microcarriers comprising soaking crosslinked dextran beads in an aqueous solution of a tertiary or quaternary amine and a base until said beads are substituted with a sufficient amount of amine moieties to produce an exchange capacity thereon of from about 0.1 to about 4.5 meq/gram of dry dextran.
Claim 12
Dependent Claim
10 words
A method of claim 7 wherein said amine comprises diethylaminoethyl.
Claim 13
Dependent Claim
11 words
A method of claim 8 wherein said base comprises sodium hydroxide.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-05 | 1985-04-06 | 1985-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-05 | 1989-04-06 | 1989-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-05 | 1993-04-06 | 1993-10-06 | $7700.00 | Window Closed / Lapsed |