US4036693A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Treatment of cell culture microcarries
Assignee: Massachusetts Institute of Technology
•
Filing: 1976-02-02
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Granted: 1977-07-19
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Expires: 1996-02-02
Abstract
Abstract A method of treating certain cell culture microcarriers to improve their performance is disclosed. In this method, positively charged microcarriers, such as those produced by reacting polydextran beads with diethylaminoethyl, are treated by contacting them with macromolecular polyanions, such as carboxymethylcellulose, prior and/or during use in cultures. Such treatment overcomes deleterious effects previously observed in attempts to use these microcarriers in cell culture systems.
Inventors
- 👤 David W. Levine
- 👤 William G. Thilly
- 👤 Daniel I. C. Wang
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:32
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12M; C12M25/00; C12M25/16; C12N2533/00; C12N2533/70
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
31 words
In the method of growing anchorage-dependent cells in a suspension of positively charged microcarriers in culture medium: the improvement comprising treating said microcarriers by contacting them with a nontoxic macromolecular polyanion.
Claim 2
Dependent Claim
31 words
In the method of growing anchorage-dependent cells in a suspension of positively charged microcarriers in culture medium: the improvement comprising treating said microcarriers by contacting them with a nontoxic macromolecular polyanion.
Claim 3
Dependent Claim
12 words
An improvement of claim 1 wherein said macromolecular polyanion is a cellulose.
Claim 4
Dependent Claim
20 words
An improvement of claim 2 wherein said macromolecular polyanion has a molecular weight of between about 600 and about 200,000.
Claim 5
Dependent Claim
12 words
An improvement of claim 3 wherein said microcarriers are positively-charged polydextran microcarriers.
Claim 6
Dependent Claim
11 words
An improvement of claim 1 wherein said macromolecular polyanion is carboxymethylcellulose.
Claim 7
Dependent Claim
11 words
An improvement of claim 4 wherein said macromolecular polyanion is carboxymethylcellulose.
Claim 8
Dependent Claim
17 words
An improvement of claim 6 wherein said microcarriers are contacted with said carboxymethylcellulose prior to cell inoculation.
Claim 9
Dependent Claim
16 words
An improvement of claim 7 wherein medium replenishments containing carboxymethylcellulose are added to said culture medium.
Claim 10
Independent Claim
23 words
A method of treating positively-charged cell culture microcarriers to enhance their cell growing characteristics comprising contacting said microcarriers with a nontoxic macromolecular polyanion.
Claim 11
Independent Claim
23 words
A method of treating positively-charged cell culture microcarriers to enhance their cell growing characteristics comprising contacting said microcarriers with a nontoxic macromolecular polyanion.
Claim 12
Dependent Claim
15 words
A method of claim 9 wherein said macromolecular polyanion is a negatively-charged polysaccharide or protein.
Claim 13
Dependent Claim
11 words
A method of claim 9 wherein said macromolecular polyanion is carboxymethylcellulose.
Claim 14
Dependent Claim
12 words
A method of claim 11 wherein said microcarriers are positively-charged polydextran microcarriers.
Claim 15
Dependent Claim
10 words
Cell culture microcarriers produced by the method of claim 9.
Claim 16
Dependent Claim
10 words
Cell culture microcarriers produced by the method of claim 12.
References Cited (Backward Citations)
US3850748A
Cited
US3910819A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-18 | 1981-01-17 | 1981-07-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-18 | 1985-01-17 | 1985-07-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-18 | 1989-01-17 | 1989-07-19 | $7700.00 | Window Closed / Lapsed |