Detection and/or determination of IgM, IgA, IgD and IgE immunoglobulins

Claim 1

Method for the detection or determination of an antigen specific immunoglobulin of a predetermined IgX class in a test medium wherein X is selected from the group consisting of M, A, D and E, comprising: (a) contacting and reacting the test medium with either insolubilized anti-IgX against the antigen specific immunoglobulin of the peculiar IgX class to be detected or determined, or antigen binding fragment of said anti-IgX under conditions suitable for forming a reaction product of said insolubilized anti-IgX bound to said IgX; (b) incubating said contacted and reacted test medium with an antigen for which the immunoglobulin to be detected or determined has specific affinity, and with a labelled antigen binding fragment of an antibody against said antigen; and (c) detecting or determining the labelling fragment, which detection or determination provides qualitative or quantitative information about the antigen specific immunoglobulin to be detected or determined.

Claim 15

A method for the detection of an IgX immunoglobulin in a serum sample containing the immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected; (b) contacting and incubating a given quantity of the liquid serum having the IgX immunoglobulin to be detected with said reagent of step (a), to form a first liquid phase and a first solid phase; (c) separating the first solid phase from the first liquid phase; (d) contacting and incubating with said first phase a given quantity of a specific binding antigen for which the IgX immunoglobulin to be detected has specific binding affinity, in order to form a second solid phase and a second liquid phase; (e) separating the solid phase of the second reaction mixture from the second liquid phase; (f) contacting and incubating said solid phase of the second reaction mixture with a given quantity of a labeled Fab-antibody against the specific binding antigen, in order to form a third solid phase and a third liquid phase; and (g) detecting the labeled activity of either the third liquid phase or the third solid phase of step (f) after separating said phases, which detection is a measure of the presence of the component to be detected.

Claim 16

A method for the detection of an IgX immunoglobulin in a serum sample containing the immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected; (b) contacting and incubating a given quantity of the liquid serum having the IgX immunoglobulin to be detected with said reagent of step (a), to form a first liquid phase and a first solid phase; (c) separating the first solid phase from the first liquid phase; (d) contacting and incubating with said first phase a given quantity of a specific binding antigen for which the IgX immunoglobulin to be detected has specific binding affinity, in order to form a second solid phase and a second liquid phase; (e) separating the solid phase of the second reaction mixture from the second liquid phase; (f) contacting and incubating said solid phase of the second reaction mixture with a given quantity of a labeled Fab-antibody against the specific binding antigen, in order to form a third solid phase and a third liquid phase; and (g) detecting the labeled activity of either the third liquid phase or the third solid phase of step (f) after separating said phases, which detection is a measure of the presence of the component to be detected.

Claim 25

A method for the detection and determination of an IgX immunoglobulin in an serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected and determined; (b) contacting and incubating a given quantity of the serum having the IgX immunoglobulin to be detected and to be determined with said reagent of step (a), whereby the reagent of step (a) is at least immunochemically equivalent to the maximum amount of IgX immunoglobulin expected, forming a reaction mixture having a first solid phase and a first liquid phase; (c) separating the first solid phase from the first liquid phase; (d) contacting and incubating with said first solid phase a given quantity of a specific binding antigen for which the IgX immunoglobulin to be detected and determined has specific binding affinity, whereby the specific binding antigen is at least immunochemically equivalent to the insolubilized anti-antibody of step (a), in order to form a second reaction mixture having a second solid phase and a second liquid phase; (e) separating the solid phase of the second reaction mixture from the second liquid phase; (f) contacting and incubating said solid phase of the second reaction mixture with a given quantity of a labeled Fab-antibody against the specific binding antigen, wherein the labeled Fab-antibody is at least immunochemically equivalent to the specific binding antigen of step (d), in order to form a third solid phase and a third liquid phase; and (g) detecting and determining the labeled activity of either the third liquid phase or the third solid phase of step (f) after separating said phases, which detection and determination is a measure of the presence and quantity of the component to be detected and determined.

Claim 26

A method for the detection and determination of an IgX immunoglobulin in an serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected and determined; (b) contacting and incubating a given quantity of the serum having the IgX immunoglobulin to be detected and to be determined with said reagent of step (a), whereby the reagent of step (a) is at least immunochemically equivalent to the maximum amount of IgX immunoglobulin expected, forming a reaction mixture having a first solid phase and a first liquid phase; (c) separating the first solid phase from the first liquid phase; (d) contacting and incubating with said first solid phase a given quantity of a specific binding antigen for which the IgX immunoglobulin to be detected and determined has specific binding affinity, whereby the specific binding antigen is at least immunochemically equivalent to the insolubilized anti-antibody of step (a), in order to form a second reaction mixture having a second solid phase and a second liquid phase; (e) separating the solid phase of the second reaction mixture from the second liquid phase; (f) contacting and incubating said solid phase of the second reaction mixture with a given quantity of a labeled Fab-antibody against the specific binding antigen, wherein the labeled Fab-antibody is at least immunochemically equivalent to the specific binding antigen of step (d), in order to form a third solid phase and a third liquid phase; and (g) detecting and determining the labeled activity of either the third liquid phase or the third solid phase of step (f) after separating said phases, which detection and determination is a measure of the presence and quantity of the component to be detected and determined.

Claim 30

A method for the detection of an IgX immunoglobulin in a serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected; (b) contacting and incubating a given quantity of the liquid serum having the IgX immunoglobulin to be detected with said reagent of step (a); (c) separating the solid phase from the liquid phase; (d) contacting and incubating with said solid phase a given quantity of a labeled reagent, which labelled reagent consists essentially of: (1) a specific binding antigen against the IgX immunoglobulin to be detected, which antigen is immunochemically bound to (2) a Fab-labeled antibody against said specific binding antigen, to form a second solid phase and a second liquid phase; and (e) detecting the labeled activity of either the second solid phase or second liquid phase of step (d) after separating said phases, which detection is a measure of the presence of the component to be detected.

Claim 31

A method for the detection of an IgX immunoglobulin in a serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected; (b) contacting and incubating a given quantity of the liquid serum having the IgX immunoglobulin to be detected with said reagent of step (a); (c) separating the solid phase from the liquid phase; (d) contacting and incubating with said solid phase a given quantity of a labeled reagent, which labelled reagent consists essentially of: (1) a specific binding antigen against the IgX immunoglobulin to be detected, which antigen is immunochemically bound to (2) a Fab-labeled antibody against said specific binding antigen, to form a second solid phase and a second liquid phase; and (e) detecting the labeled activity of either the second solid phase or second liquid phase of step (d) after separating said phases, which detection is a measure of the presence of the component to be detected.

Claim 33

A method for the detection and determination of an IgX immunoglobulin in a serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected and to be determined; (b) contacting and incubating a given quantity of the liquid sample having the IgX immunoglobulin to be detected and to be determined with said reagent of step (a), whereby the quantity of the reagent of step (a) is at least immunochemically equivalent to the maximum amount of IgX immunoglobulin expected, forming a reaction mixture having a solid phase and a liquid phase; (c) separating the solid phase from the liquid phase; (d) contacting and incubating with said solid phase a given quantity of a labeled reagent, which quantity is at least immunochemically equivalent to the insolubilized anti-antibody of step (a), which labeled reagent consists essentially of: (1) a specific binding antigen against the IgX immunoglobulin to be detected and to be determined, which antigen is immunochemically bound to (2) a Fab-labeled antibody against said specific binding antigen; to form a second solid phase and a second liquid phase; and (e) detecting and determining the labeled activity of either the second solid phase or the second liquid phase of step (d) after separating said phases, which detection and determination is a measure of the presence and quantity of the component to be detected and determined.

Claim 34

A method for the detection and determination of an IgX immunoglobulin in a serum sample containing the IgX immunoglobulin, wherein X is selected from the group consisting of M, A, D and E, comprising the steps of: (a) providing a given quantity of an insolubilized anti-antibody, or fragments thereof, against the IgX immunoglobulin to be detected and to be determined; (b) contacting and incubating a given quantity of the liquid sample having the IgX immunoglobulin to be detected and to be determined with said reagent of step (a), whereby the quantity of the reagent of step (a) is at least immunochemically equivalent to the maximum amount of IgX immunoglobulin expected, forming a reaction mixture having a solid phase and a liquid phase; (c) separating the solid phase from the liquid phase; (d) contacting and incubating with said solid phase a given quantity of a labeled reagent, which quantity is at least immunochemically equivalent to the insolubilized anti-antibody of step (a), which labeled reagent consists essentially of: (1) a specific binding antigen against the IgX immunoglobulin to be detected and to be determined, which antigen is immunochemically bound to (2) a Fab-labeled antibody against said specific binding antigen; to form a second solid phase and a second liquid phase; and (e) detecting and determining the labeled activity of either the second solid phase or the second liquid phase of step (d) after separating said phases, which detection and determination is a measure of the presence and quantity of the component to be detected and determined.