US4168261A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the purification of interferon using porous glass beads
Assignee: Stichting Rega VZW
•
Filing: 1977-05-05
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Granted: 1979-09-18
•
Expires: 1997-05-05
Abstract
Abstract An aqueous interferon solution which comprises contaminating proteins can be purified by chromatography on porous glass beads. The elution is effected at an acid pH.
Inventors
- 👤 Victor G. Edy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
35 words
A method for the purification of an aqueous interferon solution containing contaminating proteins which comprises subjecting said solution to chromatography on porous glass beads, and eluting the interferon from said beads at an acidic pH.
Claim 2
Independent Claim
35 words
A method for the purification of an aqueous interferon solution containing contaminating proteins which comprises subjecting said solution to chromatography on porous glass beads, and eluting the interferon from said beads at an acidic pH.
Claim 3
Dependent Claim
26 words
The method of claim 1, wherein the interferon is eluted from the glass beads with an aqueous solution having an acidic pH of at least 2.
Claim 4
Dependent Claim
22 words
The method of claim 2, wherein the elution is effected with a 0.1 M KCl-HCl buffer solution having a pH of 2.0.
Claim 5
Dependent Claim
28 words
The method of claim 1, wherein the glass beads are washed with a 0.01 M glycine-HCl buffer solution after adsorption of the interferon but before said elution step.
Claim 6
Dependent Claim
14 words
The method of claim 1, wherein the starting interferon solution comprises human fibroblast interferon.
Claim 7
Dependent Claim
13 words
The method of claim 1, wherein the starting interferon solution comprises mouse interferon.
Claim 8
Dependent Claim
18 words
The method of claim 1, wherein the pH of the starting interferon solution is between 7.0 and 9.2.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-09-17 | 1983-03-19 | 1983-09-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-09-17 | 1987-03-19 | 1987-09-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-09-17 | 1991-03-19 | 1991-09-18 | $7700.00 | Window Closed / Lapsed |