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US4046870A
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Assay for free thyroid hormones
Assignee: Corning Glass Works
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Filing: 1976-06-30
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Granted: 1977-09-06
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Expires: 1996-06-30
Abstract
Abstract Method for determining the concentration of free (unbound) thyroid hormones (T3 or T4) in a fluid sample. The method comprises the steps of performing an immunoassay for total T3 or total T4 in the presence and absence of a blocking agent to establish a binding differential and then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.
Inventors
- 👤 William Hertl
- 👤 Gerald Odstrchel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
75 words
A method for determining the concentration of free thyroid hormone in a fluid sample, the method comprising the steps of (1) performing separate immunoassays for thyroid hormone, one immunoassay being in the presence of a blocking agent and the other immunoassay being in the absence of a blocking agent to thereby establish a binding differential; and (2) then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.
Claim 2
Independent Claim
75 words
A method for determining the concentration of free thyroid hormone in a fluid sample, the method comprising the steps of (1) performing separate immunoassays for thyroid hormone, one immunoassay being in the presence of a blocking agent and the other immunoassay being in the absence of a blocking agent to thereby establish a binding differential; and (2) then correlating that differential with a standard curve which relates known free thyroid hormone concentrations with binding differentials.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the immunoassay is a radioimmunoassay.
Claim 4
Dependent Claim
26 words
The method of claim 2 wherein the radioimmunoassay is a solid phase radioimmunoassay using composites comprising anti-thyroxine antibodies or anti-triiodothyronine antibodies attached to a water-insoluble carrier.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein the water insoluble carrier comprises silanized glass particles.
Claim 6
Dependent Claim
38 words
The method of claim 1 wherein the binding differential is represented by the difference between the percent of thyroid hormone bound with and without blocking agent divided by the percent of thyroid hormone bound with the blocking agent.
Claim 7
Dependent Claim
13 words
The method of claim 1 wherein the fluid sample is human blood serum.
Claim 8
Dependent Claim
18 words
The method of claim 1 wherein the blocking agent is thimerosal or 8-anilino-1-naphthalensulfonic acid, or a salt thereof.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-05 | 1981-03-07 | 1981-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-05 | 1985-03-07 | 1985-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-05 | 1989-03-07 | 1989-09-06 | $7700.00 | Window Closed / Lapsed |