US3962039A
Expired / Lapsed
US
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Analytical procedure for thyroid hormones
Assignee: Center for Laboratory Medicine
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Filing: 1975-08-08
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Granted: 1976-06-08
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Expires: 1995-08-08
Abstract
Abstract Thyroid hormones in blood serum are determined by admixing a blood serum sample with pepsin, incubating the sample for a period of time to allow pepsin to digest proteins having a thyroid hormone bound thereto and release the thyroid hormone from the proteins, adjusting the resulting mixture to a pH at which pepsin is inactive and assaying the mixture for the released thyroid hormone.
Inventors
- 👤 Harold M. Bates
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:26
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/37; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/963; Y10S436/00; Y10S436/804; Y10S436/825
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
102 words
A process for the analysis of the thyroid hormone content of a blood serum sample containing a thyroid hormone bound to blood serum proteins, which comprises admixing with a sample of blood serum an effective amount of pepsin at a pH of about 1.0, incubating the sample for a period of time and at a temperature sufficient to allow the pepsin to digest the proteins to which the thyroid hormone is bound and to release the thyroid hormone from said proteins, adjusting the mixture to a pH at which the pepsin is inactive, and assaying the mixture for the released thyroid hormone.
Claim 2
Dependent Claim
102 words
A process for the analysis of the thyroid hormone content of a blood serum sample containing a thyroid hormone bound to blood serum proteins, which comprises admixing with a sample of blood serum an effective amount of pepsin at a pH of about 1.0, incubating the sample for a period of time and at a temperature sufficient to allow the pepsin to digest the proteins to which the thyroid hormone is bound and to release the thyroid hormone from said proteins, adjusting the mixture to a pH at which the pepsin is inactive, and assaying the mixture for the released thyroid hormone.
Claim 3
Dependent Claim
12 words
A process according to claim 1 wherein said thyroid hormone is thyroxine.
Claim 4
Dependent Claim
12 words
A process according to claim 1 wherein said thyroid hormone is triiodothyronine.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-08 | 1979-12-08 | 1980-06-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-08 | 1983-12-08 | 1984-06-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-08 | 1987-12-08 | 1988-06-08 | $7700.00 | Window Closed / Lapsed |