US3966897A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Medium for use in bioassay and method of using same
Assignee: Marine Colloids Inc
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Filing: 1973-04-02
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Granted: 1976-06-29
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Expires: 1993-04-02
Abstract
Abstract A hydratable or hydrated gel medium for use in bioassay of a test substance contains fixed in a localized zone a reagent for the test substance. Assays are conducted rapidly by diffusing the test substance followed by an indicator substance also reactive with the reagent into the zone and measuring a change in characteristics within the zone caused by the reaction of the indicator substance.
Inventors
- 👤 Donald W. Renn
- 👤 Calvin A. Saravis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/558; G01N33/559; C; C12; C12Q; C12Q1/00; Y; Y10; Y10S; Y10S436/00; Y10S436/805; Y10S436/82
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
66 words
A medium for use in bioassay diffusion processes with test substances, said medium comprising a layer of dry material hydratable to a gel through which said test substance can diiffuse, and fixed to a restricted localized zone of said layer against diffusive movement therethrough a reagent capable of reaction with said test substance, said reagent being confined to said zone which is smaller than said layer.
Claim 2
Independent Claim
66 words
A medium for use in bioassay diffusion processes with test substances, said medium comprising a layer of dry material hydratable to a gel through which said test substance can diiffuse, and fixed to a restricted localized zone of said layer against diffusive movement therethrough a reagent capable of reaction with said test substance, said reagent being confined to said zone which is smaller than said layer.
Claim 3
Independent Claim
63 words
A medium for use in bioassay diffusion processes with test substances, said medium comprising a layer of hydrated gel material through which said test substance can diffuse, and fixed to a restricted localized zone of said layer against diffusive movement therethrough a reagent capable of reaction with said test substance, said reagent being confined to said zone which is smaller than said layer.
Claim 4
Independent Claim
63 words
A medium for use in bioassay diffusion processes with test substances, said medium comprising a layer of hydrated gel material through which said test substance can diffuse, and fixed to a restricted localized zone of said layer against diffusive movement therethrough a reagent capable of reaction with said test substance, said reagent being confined to said zone which is smaller than said layer.
Claim 5
Dependent Claim
22 words
A medium as claimed in claim 1 in which said reagent is an antibody, an antigen, an enzyme, or an enzyme substrate.
Claim 6
Dependent Claim
13 words
A medium as claimed in claim 1 in which said reagent is proteinaceous.
Claim 7
Dependent Claim
16 words
A medium as claimed in claim 1 in which said hydratable material is an organic polymer.
Claim 8
Dependent Claim
36 words
A medium as claimed in claim 1 in which said hydratable material is an organic hydroxy-containing polymer, and said reagent is proteinaceous and is bound to said polymer by means of a cyanogen halide bonding agent.
Claim 9
Dependent Claim
34 words
A medium as claimed in claim 1 in which said hydratable material is a polymer of acrylamide, and said reagent is proteinaceous and is bound to said polymer by means of glutaraldehyde bonding agent.
Claim 10
Dependent Claim
22 words
A medium as claimed in claim 1 in which said layer is a cellulosic material, a polysaccharide or a polymer of acrylamide.
Claim 11
Dependent Claim
94 words
The method of conducting a bioassay of a test substance which comprises providing a medium as claimed in claim 2, applying to said medium a sample of said test substance and an indicator substance also capable of reacting with said reagent, at least said indicator substance being applied to said medium at a position spaced from the margin of said zone, causing said substances to undergo diffusion into said zone to react sequentially with said reagent, and subsequently measuring the change in characteristics within said zone caused by the reaction of said indicator substance.
Claim 12
Dependent Claim
43 words
The method as claimed in claim 9 in which said indicator substance is radioactively labelled and the characteristic measured is radioactivity, and including the additional step of removing from said zone by diffusion any excess of indicator substance before said radioactivity is measured.
Claim 13
Dependent Claim
88 words
The method as claimed in claim 10 in which the test substance is applied to said medium at a position spaced from the margin of said zone and the medium is subjected to electrophoresis to cause said test substance to undergo diffusion into said zone, and an indicator substance in an amount in excess of that capable of reacting with said reagent is applied to said medium at a position spaced from the margin of said zone and caused to undergo diffusion sequentially into said zone by electrophoresis.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-29 | 1979-12-29 | 1980-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-29 | 1983-12-29 | 1984-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-29 | 1987-12-29 | 1988-06-29 | $7700.00 | Window Closed / Lapsed |