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US3578604A Expired / Lapsed US ⏱️ 0.0 years remaining

Preparation of mixed gels of acrylamide and agarose or agar-agar

Assignee: Centre National de la Recherche Scientifique
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Filing: 1966-11-29
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Granted: 1971-05-11
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Expires: 1986-11-29
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Abstract

Abstract MIXED GELS OF ACRYLAMIDE AND A SUBSTANCE SELECTED FROM THE GROUP CONSISTING OF AGAROSE AND AGAR-AGAR, WHICH GELS'' ARE ESPECIALLY SUITABLE IN THE ELECTROPHORETIC SEPARATION OF NATURAL PRODUCTS AS MOLECUALR SIEVES AND FILTERS, ARE PREPARED BY PROVIDING HEATED, BUFFERED SOLUTIONS OF (1) MELTED AGAROSE OF AGAR-AGAR, AND (2) ACRYLAMIDE WITH N,N''-METHYLENE-BISACRYLAMIDE, ADDING AMMONIUM PERSULFATE AND N,N''N'',-TETRAMETHYL-ETHYLENE DIAMINE TO SOLUTION (1), RAPIDLY MIXING SO-TREATED SOLUTION (1) AND SOLUTION (2), IMMEDIATELY CASTING THE MIXTURE IN AN OVEN AT A TEMPERATURE ABOVE 50*C ., AND LETTING THE ACRYLAMIDE GEL FORM AND COOL TO ROOM TEMPERATURE.

Inventors

  • 👤 Jose Uriel

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 16:32
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704; G01N27/44747; B; B01; B01J; B01J20/00; B01J20/22

Claims Analysis

Total Claims: 0
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References Cited (Backward Citations)

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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1974-05-10 1974-11-09 1975-05-11 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1978-05-10 1978-11-09 1979-05-11 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1982-05-10 1982-11-09 1983-05-11 $7700.00 Window Closed / Lapsed

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