US3964973A
Expired / Lapsed
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Preparation of insoluble biologically active compounds
Assignee: Czech Academy of Sciences
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Filing: 1974-01-30
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Granted: 1976-06-22
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Expires: 1994-01-30
Abstract
Abstract Biologically active compounds such as enzymes are insolubilized by hydrophilizing a macroporous substantially hydrophobic carrier by hydrolysis of a thin surface layer of the carrier to form free carboxylic acid groups, and thereafter activating the carrier and reacting the activated carrier with a biologically active compound.
Inventors
- 👤 Jiri Hradil
- 👤 Jiri Coupek
- 👤 Miroslava Krivakova
- 👤 Jiri Stamberg
- 👤 Arthur Stoy
- 👤 Jaroslava Turkova
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:32
CPC Classifications
C; C08; C08F; C08F8/00; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
165 words
A method of preparing a water insoluble biologically active product comprising subjecting a macroporous substantially hydrophobic polymeric carrier comprising a homopolymer or copolymer derived from (1) at least one acrylic monomer selected from the group consisting of alkylenediacrylates and dimethacrylates, oligo- and polyglycoldiacrylates and dimethacrylates, alkylenediacrylamides and methacrylamides, hydroxyalkylacrylates and methacrylates, oligo- and polyglycol monoacrylates and monomethacrylates, acrylonitrile and methacrylonitrile, or (2) a copolymer of at least one monomer of group (1) above with a vinyl monomer selected from the group consisting of vinylaniline, vinylpyridine and vinylcarbazol, to a superficial hydrolysis treatment with an aqueous alkaline medium to form free carboxylic acid groups in a thin surface layer of said polymeric carrier, subjecting the resulting superficially hydrolyzed polymeric carrier to an activation treatment comprising converting said free carboxylic acid groups to active carboxylic acid anhydride groups, and contacting the resulting activated polymeric carrier with a biologically active protein compound capable of being insolubilized and bound by reaction with said anhydride groups in said polymeric carrier.
Claim 2
Independent Claim
165 words
A method of preparing a water insoluble biologically active product comprising subjecting a macroporous substantially hydrophobic polymeric carrier comprising a homopolymer or copolymer derived from (1) at least one acrylic monomer selected from the group consisting of alkylenediacrylates and dimethacrylates, oligo- and polyglycoldiacrylates and dimethacrylates, alkylenediacrylamides and methacrylamides, hydroxyalkylacrylates and methacrylates, oligo- and polyglycol monoacrylates and monomethacrylates, acrylonitrile and methacrylonitrile, or (2) a copolymer of at least one monomer of group (1) above with a vinyl monomer selected from the group consisting of vinylaniline, vinylpyridine and vinylcarbazol, to a superficial hydrolysis treatment with an aqueous alkaline medium to form free carboxylic acid groups in a thin surface layer of said polymeric carrier, subjecting the resulting superficially hydrolyzed polymeric carrier to an activation treatment comprising converting said free carboxylic acid groups to active carboxylic acid anhydride groups, and contacting the resulting activated polymeric carrier with a biologically active protein compound capable of being insolubilized and bound by reaction with said anhydride groups in said polymeric carrier.
Claim 3
Dependent Claim
26 words
A method as defined in claim 1 wherein said hydrolysis treatment is carried out at a temperature of 60°C. to 135°C. for 30 to 120 minutes.
Claim 4
Dependent Claim
22 words
A method as defined in claim 2 wherein said aqueous alkaline medium contains sodium hydroxide at a concentration of 2M to 10M.
Claim 5
Dependent Claim
38 words
A method as defined in claim 3 wherein said activation treatment comprises heating the superifically hydrolyzed polymeric carrier to a temperature of 200°C. to 250°C. for 30 to 300 minutes at a pressure of 0.2 to 0.01 Torr.
Claim 6
Independent Claim
178 words
A method of preparing a water insoluble active product comprising subjecting a macroporous substantially hydrophobic polymeric carrier comprising a homopolymer or copolymer derived from (1) at least one acrylic monomer selected from the group consisting of alkylenediacrylates and dimethacrylates, oligo- and polyglycoldiacrylates and dimethacrylates, alkylenediacrylamides and methacrylamides, hydroxyalkylacrylates and methacrylates, oligo- and polyglycol monoacrylates and monomethacrylates, acrylonitrile and methacrylonitrile or (2) a copolymer of at least one monomer of group (1) above with a vinyl monomer selected from the group consisting of vinylaniline, vinylpyridine and vinylcarbazol, to a superficial hydrolysis treatment with an aqueous alkaline medium to form free carboxylic acid groups in a thin surface layer of said polymeric carrier, activating the resulting superficially thin layer of said hydrolyzed polymeric carrier by treating the superficially hydrolyzed polymeric carrier with a member of the group consisting of aceticanhydride and thionyl chloride to provide said surface layer with anhydride groups and contacting the resulting activated polymeric carrier with a biologically active protein compound capable of being insolubilized and bound by reaction with said anhydride groups in said polymeric carrier.
Claim 7
Independent Claim
178 words
A method of preparing a water insoluble active product comprising subjecting a macroporous substantially hydrophobic polymeric carrier comprising a homopolymer or copolymer derived from (1) at least one acrylic monomer selected from the group consisting of alkylenediacrylates and dimethacrylates, oligo- and polyglycoldiacrylates and dimethacrylates, alkylenediacrylamides and methacrylamides, hydroxyalkylacrylates and methacrylates, oligo- and polyglycol monoacrylates and monomethacrylates, acrylonitrile and methacrylonitrile or (2) a copolymer of at least one monomer of group (1) above with a vinyl monomer selected from the group consisting of vinylaniline, vinylpyridine and vinylcarbazol, to a superficial hydrolysis treatment with an aqueous alkaline medium to form free carboxylic acid groups in a thin surface layer of said polymeric carrier, activating the resulting superficially thin layer of said hydrolyzed polymeric carrier by treating the superficially hydrolyzed polymeric carrier with a member of the group consisting of aceticanhydride and thionyl chloride to provide said surface layer with anhydride groups and contacting the resulting activated polymeric carrier with a biologically active protein compound capable of being insolubilized and bound by reaction with said anhydride groups in said polymeric carrier.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-06-22 | 1979-12-22 | 1980-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-06-22 | 1983-12-22 | 1984-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-06-22 | 1987-12-22 | 1988-06-22 | $7700.00 | Window Closed / Lapsed |