US3821083A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of immobilized enzymes
Assignee: Nestec SA
•
Filing: 1972-06-06
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Granted: 1974-06-28
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Expires: 1992-06-06
Abstract
Abstract A water-insoluble enzyme product comprises an enzyme bonded to aldehyde groups of a water-insoluble polymer coated on an inert carrier. Polyacrolein is the preferred polymer, and enzymes include papain and trypsin. Processes for preparing the products are also described.
Inventors
- 👤 Leemputten E Van
- 👤 M Horisberger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:54
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12N11/02; C12N11/08; C12N11/082
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
18 words
A process according to claim 1 in which the acetal of polyacrolein is the methyl-acetal or the butyl-acetal.
Claim 2
Dependent Claim
29 words
A process according to claim 1 in which the enzyme contains at least one amino group capable of reacting with at least one free aldehyde group of the polymer.
Claim 3
Dependent Claim
14 words
A process according to claim 3 in which the enzyme is papain or trypsin.
Claim 4
Dependent Claim
22 words
A process according to claim 1 in which the reaction between the enzyme and the polymer is effected in a buffer solution.
Claim 5
Dependent Claim
18 words
A process according to claim 5 in which the buffer solution has a pH of 7 to 8.
Claim 6
Dependent Claim
11 words
A water-insoluble enzyme product produced by the process of claim 1.
Claim 7
Dependent Claim
14 words
A product according to claim 7 in which the enzyme is papain or trypsin.
Claim 8
Dependent Claim
18 words
A product according to claim 7 in which the acetal of polyacrolein is the methyl-acetal or the butyl-acetal.
Claim 9
Dependent Claim
20 words
A product according to claim 7 in which enzyme is bonded essentially only to superficial aldehyde groups of the layer.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-27 | 1977-12-27 | 1978-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-27 | 1981-12-27 | 1982-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-27 | 1985-12-27 | 1986-06-28 | $7700.00 | Window Closed / Lapsed |