US3645852A
Expired / Lapsed
US
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Method of binding water-soluble proteins and water-soluble peptides to water-insoluble polymers using cyanogen halide
Assignee: Pfizer Health AB
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Filing: 1970-03-06
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Granted: 1972-02-29
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Expires: 1990-03-06
Abstract
Abstract Cyanogen halides are employed for binding water-soluble proteins and water-soluble peptides containing a primary or secondary amino group to a water-insoluble polymer containing one or more hydroxyl and/or primary and/or secondary amino groups. The invention is useful, among other things, for binding watersoluble enzymes to water-insoluble polymers while preserving the activity of the enzyme.
Inventors
- 👤 Rolf E A V Axen
- 👤 Jerker Olof Porath
- 👤 Erik Sverker Ernbach
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 22:01
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/817; Y10S436/818; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
29 words
thereafter reacting the water-insoluble polymer obtained from step (1) with a member selected from the group consisting of water-soluble proteins and water-soluble peptides of the type specified in (b).
Claim 2
Dependent Claim
40 words
The method according to claim 1, wherein said water-insoluble polymer is selected from the group consisting of water-insoluble polysaccharides and water-insoluble polysaccharide derivatives containing a member selected from the group consisting of hydroxyl groups and primary and secondary amino groups.
Claim 3
Dependent Claim
18 words
The method as set forth in claim 1, wherein said water-insoluble polymer is insoluble but swellable in water.
Claim 4
Dependent Claim
18 words
The method as claimed in claim 1, wherein said water-insoluble polymer is a cross-linked three-dimensional hydroxyl group-containing network.
Claim 5
Dependent Claim
17 words
The method as claimed in claim 1, wherein the polymer is a copolymer of dextran with epichlorohydrin.
Claim 6
Dependent Claim
15 words
The method as set forth in claim 1, wherein the water-soluble peptide is an enzyme.
Claim 7
Dependent Claim
25 words
The method as set forth in claim 1, wherein the member selected from the group consisting of water-soluble proteins and water-soluble peptides is an antibody.
Claim 8
Dependent Claim
24 words
The method as claimed in claim 1, wherein the member selected from the group consisting of water-soluble proteins and water-soluble peptides is an antigen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |