Patent US3850748A successfully refreshed from Google Patents.
US3850748A
Expired / Lapsed
US
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Method of producing animal cells in suspension culture
Assignee: Eli Lilly and Co
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Filing: 1973-06-28
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Granted: 1974-11-26
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Expires: 1993-06-28
Abstract
Abstract A new and superior method of producing cells in suspension culture is carried out by culturing the cells in a new culture medium under suitable agitation in a culture vessel which presents resinous, organic, polymeric surfaces to the medium. Blood serum is an ingredient of the medium. The temperature and pH of the medium are those regularly used in cell culture. The cells remain in the culture without passaging, cells being removed as necessary to keep the cell population at about 105 to about 106 cells/ml. Medium is periodically removed and replaced with fresh medium, and aggregated cells are periodically dispersed.
Inventors
- 👤 R Cook
- 👤 F Counter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 00:57
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
28 words
AGITATING THE MEDIUM SUFFICIENTLY TO KEEP THE CELLS IN SUSPENSION, BUT NOT SO VIGOROUSLY AS TO CAUSE FOAMING OF THE MEDIUM OR DENATURATION OF COMPONENTS OF THE MEDIUM;
Claim 2
Dependent Claim
28 words
AGITATING THE MEDIUM SUFFICIENTLY TO KEEP THE CELLS IN SUSPENSION, BUT NOT SO VIGOROUSLY AS TO CAUSE FOAMING OF THE MEDIUM OR DENATURATION OF COMPONENTS OF THE MEDIUM;
Claim 3
Independent Claim
31 words
A METHOD OF PRODUCING ANIMAL CELLS IN SUSPENSION CULTURE WHICH COMPRISES: A. ADDING A POPULATION OF AT LEAST ABOUT 10**-5 OF THE CELLS PER ML. TO A SUSPENSION CULTURE MEDIUM COMPRISING:
Claim 4
Independent Claim
31 words
A METHOD OF PRODUCING ANIMAL CELLS IN SUSPENSION CULTURE WHICH COMPRISES: A. ADDING A POPULATION OF AT LEAST ABOUT 10**-5 OF THE CELLS PER ML. TO A SUSPENSION CULTURE MEDIUM COMPRISING:
Claim 5
Dependent Claim
24 words
MAINTAINING THE TEMPERATURE OF THE MEDIUM AT ABOUT 32*C. TO ABOUT 41*C.; 3.MAINTAINING THE PH OF THE MEDIUM AT ABOUT 6.8 TO ABOUT 7.2;
Claim 6
Dependent Claim
24 words
MAINTAINING THE TEMPERATURE OF THE MEDIUM AT ABOUT 32*C. TO ABOUT 41*C.; 3.MAINTAINING THE PH OF THE MEDIUM AT ABOUT 6.8 TO ABOUT 7.2;
Claim 7
Dependent Claim
15 words
A method of claim 1 wherein the polymeric surfaces comprise polycarbonate, polypropylene, or polytetrafluoroethylene polymers.
Claim 8
Dependent Claim
12 words
maintaining the temperature of the medium at about 32*C. to about 41*C.;
Claim 9
Dependent Claim
12 words
maintaining the temperature of the medium at about 32*C. to about 41*C.;
Claim 10
Dependent Claim
12 words
maintaining the pH of the medium at about 6.8 to about 7.2;
Claim 11
Dependent Claim
12 words
maintaining the pH of the medium at about 6.8 to about 7.2;
Claim 12
Dependent Claim
15 words
A method of claim 2 wherein the temperature is from about 35*C. to about 38*C.
Claim 13
Dependent Claim
11 words
A method of claim 3 wherein the blood serum is bovine.
Claim 14
Dependent Claim
6 words
PERIODICALLY REMOVING PART OF THE MEDIUM;
Claim 15
Dependent Claim
6 words
PERIODICALLY REMOVING PART OF THE MEDIUM;
Claim 16
Dependent Claim
6 words
periodically removing part of the medium;
Claim 17
Dependent Claim
6 words
periodically removing part of the medium;
Claim 18
Dependent Claim
8 words
periodically replacing the medium removed with fresh medium;
Claim 19
Dependent Claim
8 words
periodically replacing the medium removed with fresh medium;
Claim 20
Dependent Claim
8 words
PERIODICALLY REPLACING THE MEDIUM REMOVED WITH FRESH MEDIUM;
Claim 21
Dependent Claim
8 words
PERIODICALLY REPLACING THE MEDIUM REMOVED WITH FRESH MEDIUM;
Claim 22
Dependent Claim
21 words
A method of claim 1 wherein the concentration range of the blood serum in the suspension culture medium is 50-150 ml./l.
Claim 23
Dependent Claim
15 words
A method of claim 5 wherein the polymeric surfaces comprise polycarbonate, polypropylene, or polytetrafluoroethylene polymers.
Claim 24
Dependent Claim
6 words
PERIODICALLY DISPERSING AGGREGATES OF CELLS; AND
Claim 25
Dependent Claim
6 words
PERIODICALLY DISPERSING AGGREGATES OF CELLS; AND
Claim 26
Dependent Claim
6 words
periodically dispersing aggregates of cells; and
Claim 27
Dependent Claim
6 words
periodically dispersing aggregates of cells; and
Claim 28
Dependent Claim
21 words
periodically removing a quantity of cells sufficient to maintain the cell population at about 105 to about 106 cells per milliliter.
Claim 29
Dependent Claim
21 words
periodically removing a quantity of cells sufficient to maintain the cell population at about 105 to about 106 cells per milliliter.
Claim 30
Dependent Claim
21 words
PERIODICALLY REMOVING A QUANTITY OF CELLS SUFFICIENT TO MAINTAIN THE CELL POPULATION AT ABOUT 10**-5 TO ABOUT 10**-6 CELLS PER MILLILITER.
Claim 31
Dependent Claim
21 words
PERIODICALLY REMOVING A QUANTITY OF CELLS SUFFICIENT TO MAINTAIN THE CELL POPULATION AT ABOUT 10**-5 TO ABOUT 10**-6 CELLS PER MILLILITER.
Claim 32
Dependent Claim
15 words
A method of claim 6 wherein the temperature is from about 35*C. to about 38*C.
Claim 33
Dependent Claim
11 words
A method of claim 7 wherein the blood serum is bovine.
Claim 34
Dependent Claim
11 words
A method of claim 5 wherein the suspension culture medium comprises:
Claim 35
Dependent Claim
15 words
A method of claim 9 wherein the polymeric surfaces comprise polycarbonate, polypropylene, or polytetrafluoroethylene polymers.
Claim 36
Dependent Claim
15 words
A method of claim 10 wherein the temperature is from about 35*C. to about 38*C.
Claim 37
Dependent Claim
11 words
A method of claim 11 wherein the blood serum is bovine.
References Cited (Backward Citations)
US3073746A
Cited
US3122476A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-25 | 1978-05-27 | 1978-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-25 | 1982-05-27 | 1982-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-25 | 1986-05-27 | 1986-11-26 | $7700.00 | Window Closed / Lapsed |