US3122476A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Tissue culture medium
Assignee: Hyland Laboratories Inc
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Filing: 1960-06-21
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Granted: 1964-02-25
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Expires: 1980-06-21
Abstract
No abstract record available.
Inventors
- 👤 Lawrence E Gaeta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 00:58
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
105 words
A METHOD OF PREPARING A NEWBORN CALF SERUM USEFUL IN THE PREPARATION OF A GROWTH MEDIUM FOR NORMAL HUMAN AND ANIMAL CELLS, WHICH COMPRISES COLLECTING THE BLOOD OF NEWBORN CALVES, POOLING THE SERUM WHICH CONTAINS GAMMA GLOBULIN, REMOVING FIBRIN FROM THE SERUM, CHILLING THE SERUM TO ABOUT 0*C. AND ADDING CONCENTRATED ETHANAL TO THE SERUM TO ADJUST THE CONCENTRATION OF THE MIXTURE TO 20% ETHANOL, ADJUSTING THE PH TO ABOUT 7 AND COOLING AND AGITATING THE MIXTURE TO PRECIPITATE ALL THE GAMMA GLOBULIN PRESENT THEN REMOVING SAID PRECIPITATED GAMMA GLOBULIN TO OBTAIN A CLEAR SERUM WHICH IS NON TOXIC TO NORMAL HUMAN AND ANIMAL CELLS.
Claim 2
Independent Claim
105 words
A METHOD OF PREPARING A NEWBORN CALF SERUM USEFUL IN THE PREPARATION OF A GROWTH MEDIUM FOR NORMAL HUMAN AND ANIMAL CELLS, WHICH COMPRISES COLLECTING THE BLOOD OF NEWBORN CALVES, POOLING THE SERUM WHICH CONTAINS GAMMA GLOBULIN, REMOVING FIBRIN FROM THE SERUM, CHILLING THE SERUM TO ABOUT 0*C. AND ADDING CONCENTRATED ETHANAL TO THE SERUM TO ADJUST THE CONCENTRATION OF THE MIXTURE TO 20% ETHANOL, ADJUSTING THE PH TO ABOUT 7 AND COOLING AND AGITATING THE MIXTURE TO PRECIPITATE ALL THE GAMMA GLOBULIN PRESENT THEN REMOVING SAID PRECIPITATED GAMMA GLOBULIN TO OBTAIN A CLEAR SERUM WHICH IS NON TOXIC TO NORMAL HUMAN AND ANIMAL CELLS.
Claim 3
Dependent Claim
56 words
A NEWBORN CALF SERUM USEFUL IN THE PREPARATION OF TISSUE CULTURE MEDIA FOR THE GROWTH OF NORMAL HUMAN AND ANIMAL CELLS IN VITRO, SAID NEWBORN CALF SERUM CONTAINING ABOUT 44% OF A NONTOXIC SUBSTANCE HAVING THE CELL MOBILITY OF GAMMA GLOBULIN AS DETERMINED BY ELECTROPHORETIC ANALYSIS WHICH SERUM IS PREPARED BY THE METHOD OF CLAIM 1.
References Cited (Backward Citations)
US2543215A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1967-02-24 | 1967-08-26 | 1968-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1971-02-24 | 1971-08-26 | 1972-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1975-02-24 | 1975-08-26 | 1976-02-25 | $7700.00 | Window Closed / Lapsed |