US3836433A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fixation of nitrogenous materials
Assignee: RECH APPLIC SOC GEN DE
•
Filing: 1972-04-13
•
Granted: 1974-09-17
•
Expires: 1992-04-13
Abstract
Abstract Dialdehydes, e.g., glyoxal or glutaraldehyde are used to fix nitrogenous materials, e.g., amino acids, proteins, enzymes, antigens, hormones, etc., to insoluble supports which form gels in aqueous media, e.g., polyacrylamide or polysaccharides such as agar-agar.
Inventors
- 👤 P Wirth
- 👤 R Tixier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:59
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
15 words
A process as claimed in claim 1 in which the polysaccharide is agar or agarose.
Claim 2
Dependent Claim
16 words
A process as claimed in claim 1 in which the nitrogenous substance is a natural compound.
Claim 3
Dependent Claim
55 words
A process as claimed in claim 3 in which the nitrogenous substance is a natural compound, such as an amino acid, a polypeptide or a compound containing a proteic moiety or an amino or amido function such as an enzyme, enzyme inhibitor, anti-body, anti-gen, polypeptide hormone, proteic hormone or a protein such as a concanavaline.
Claim 4
Dependent Claim
25 words
A process as claimed in claim 1 in which the dialdehyde is of the formula: OHC-R-CHO in which R is an aliphatic or aromatic chain.
Claim 5
Dependent Claim
26 words
A process as claimed in claim 5 in which R is a polymethylene chain of the formula -(CH2)n in which n is from 0 to 6.
Claim 6
Dependent Claim
15 words
A process as claimed in claim 5 in which the dialdehyde is glutaraldehyde or glyoxal.
Claim 7
Dependent Claim
46 words
A process as claimed in claim 1 which comprises, in the first stage, reacting beads of the gel with an excess of a dialdehyde compound in aqueous solution, and, in a second stage, reacting the nitrogenous substance with the free aldehyde groups attached to the support.
Claim 8
Dependent Claim
21 words
A process as claimed in claim 2 wherein the support is an agarose-acrylamide gel and the nitrogenous substance in an enzyme.
Claim 9
Dependent Claim
27 words
A process as claimed in claim 9 in which the enzyme is trypsine, chymotrypsine, a pancreatic protease, a papaya juice protease, alpha-amylase, Urease, arginase, lysozyme or cytochrome.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-09-16 | 1978-03-18 | 1978-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-09-16 | 1982-03-18 | 1982-09-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-09-16 | 1986-03-18 | 1986-09-17 | $7700.00 | Window Closed / Lapsed |