Patent US2357833A successfully refreshed from Google Patents.
US3767531A
Expired / Lapsed
US
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Preparation of insolubilized enzymes
Assignee: United States Department of Agriculture
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Filing: 1972-02-14
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Granted: 1973-10-23
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Expires: 1992-02-14
Abstract
Abstract Insolubilized but active enzymes are prepared by reacting an enzyme with a phenol-formaldehyde resin and glutaraldehyde.
Inventors
- 👤 A Olson
- 👤 W Stanley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:29
CPC Classifications
C; C08; C08G; C08G8/00; C08G8/28; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/089; C12N11/091
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
60 words
A process for preparing an insoluble but active enzyme which comprises: a. dissolving a soluble enzyme in water, b. mixing the resulting solution with granules of solid phenolformaldehyde resin to cause the enzyme to be adsorbed by the resin, c. contacting the resin containing adsorbed enzyme with an aqueous solution of glutaraldehyde, and d. separating the resulting enzyme-resin-glutaraldehyde reaction product.
Claim 2
Dependent Claim
60 words
A process for preparing an insoluble but active enzyme which comprises: a. dissolving a soluble enzyme in water, b. mixing the resulting solution with granules of solid phenolformaldehyde resin to cause the enzyme to be adsorbed by the resin, c. contacting the resin containing adsorbed enzyme with an aqueous solution of glutaraldehyde, and d. separating the resulting enzyme-resin-glutaraldehyde reaction product.
Claim 3
Dependent Claim
19 words
An insoluble but active enzyme comprising a complex of a soluble enzyme and a phenol-formaldehyde resin, cross-linked with glutaraldehyde.
Claim 4
Dependent Claim
19 words
An insoluble but active enzyme comprising a complex of a soluble enzyme and a phenol-formaldehyde resin, cross-linked with glutaraldehyde.
Claim 5
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a sugar-hydrolyzing enzyme.
Claim 6
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a glucose-oxidizing enzyme.
Claim 7
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a protein-degrading enzyme.
Claim 8
Dependent Claim
13 words
The product of claim 2 wherein the enzyme is a hydrogen peroxide-degrading enzyme.
Claim 9
Dependent Claim
12 words
The product of claim 2 wherein the enzyme is a starch-hydrolyzing enzyme.
References Cited (Backward Citations)
US3705084A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-22 | 1977-04-23 | 1977-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-22 | 1981-04-23 | 1981-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-22 | 1985-04-23 | 1985-10-23 | $7700.00 | Window Closed / Lapsed |