Osteogenic composition and implant containing same

Claim 2

The osteogenic composition of Claim 1 which is in viscous liquid form.

Claim 3

The osteogenic composition of Claim 1 wherein in step (b), the demineralized bone matrix is converted to a pulp.

Claim 4

The osteogenic composition of Claim 1 wherein the demineralized bone matrix contains not greater than about 10 weight percent calcium.

Claim 5

The osteogenic composition of Claim 4 wherein the demineralized bone matrix contains not greater than about 1 weight percent calcium.

Claim 6

The osteogenic composition of Claim 5 wherein the demineralized bone matrix contains not greater than about 0.5 weight percent calcium.

Claim 7

The osteogenic composition of Claim 1 wherein heating is carried out at a temperature of from about 30 to about 75°C.

Claim 8

The osteogenic composition of Claim 7 wherein heating is carried out at a temperature of from about 35 to about 60°C.

Claim 9

The osteogenic composition of Claim 8 wherein heating is carried out at a temperature of from about 40 to about 55°C.

Claim 10

The osteogenic composition of Claim 1 wherein heating is carried out for a period of from about 5 minutes to about 10 hours.

Claim 11

The osteogenic composition of Claim 1 containing demineralized bone powder.

Claim 12

The osteogenic composition of Claim 1 containing at least one ingredient selected from the group consisting of antiviral agent, antimicrobial agent, antibiotic agent, amino acid, peptide, vitamin, inorganic element, hormone, endocrine tissue, synthesizer, enzyme, polymer-cell scaffolding agent with parenchymal cells, angiogenic drug, polymeric drug carrier, collagen lattice, antigenic agent, cytoskeletal agent, cartilage fragment, living cell, natural extract, tissue transplant, bioadhesive, biologically active component, mesenchymal agent, bone digester, antitumor agent, cellular attractant, cellular attachment agent, immunosuppressant, nucleic acid and penetration enhancer.

Claim 13

The osteogenic composition of Claim 1 adhered to the surface of and/or incorporated within a surgical implant device.

Claim 14

The osteogenic composition of Claim 13 wherein the surgical implant device is selected from the group consisting of donor bone tissue, an osteoprosthetic device, an orthopedic fracture wrapping and a collagen-coated microspherical filler.

Claim 15

The osteogenic composition of Claim 1, additionally comprising: d) treating the liquid composition produced in step (c) to obtain the osteogenic composition in solid form by adding organic solvent to the liquid composition, whereby the osteogenic composition precipitates out of solution in solid form.

Claim 16

The osteogenic composition of Claim 15, additionally comprising: titrating the liquid composition produced in step (c), before carrying out step (d), to remove excess acid.

Claim 17

The osteogenic composition of Claim 15, wherein the precipitated solid composition is further dissolved in solution, whereby the osteogenic composition is in injectable form.

Claim 18

The osteogenic composition of Claim 17, which is in a form suitable for delivery by percutaneous injection to a fracture delivery site, for direct injection into an osseous defect resulting from periodontal disease (subgingival injection), or as a general injectable for stimulating bone growth to counteract bone loss due to injury or disease.

Claim 19

The osteogenic composition of Claim 1, additionally comprising: d) treating the liquid composition produced in step (c) to obtain the osteogenic composition in solid form, by first titrating the liquid composition produced in step (c) to remove excess acid, then placing the titrated composition in a dialysis bag and then carrying out dialysis, whereby the osteogenic composition remains in the dialysis bag in solid form.

Claim 20

The osteogenic composition of Claim 19, wherein the dialyzed solid composition is further dissolved in solution, whereby the osteogenic composition is in injectable form.

Claim 21

The osteogenic composition of Claim 21, which is in a form suitable for delivery by percutaneous injection to a fracture delivery site, for direct injection into an osseous defect resulting from periodontal disease (subgingival injection) or as a general injectable for stimulating bone growth to counteract bone loss due to injury or disease.

Claim 22

The osteogenic composition of Claim 1 wherein said acid-promoted cleavage step (b) is carried out simultaneously with said bone demineralizing step (a).

Claim 23

The osteogenic composition of Claim 1 wherein said acid-promoted cleavage step (b) is separately carried out after said demineralizing step (a) has been completed.

Claim 24

The osteogenic composition of Claim 1 wherein said heating step (c) is carried out simultaneously with or subsequent to said acid-promoted cleavage step (b).

Claim 25

The osteogenic composition of Claim 1 containing hydroxyapatite.

Claim 26

The osteogenic composition of Claim 1 containing non-demineralized bone powder.

Claim 27

The osteogenic composition of Claim 1 containing at least one ingredient selected from the group consisting of bone morphogenic agents and growth factors.

Claim 28

The osteogenic composition of Claim 27 containing at least one ingredient selected from the group consisting of platelet derived growth factor, basic fibroblast growth factor, transforming growth factor beta, insulin-like growth factor, and epidermal growth factor.

Claim 29

A process of making an osteogenic composition, which comprises the steps of: a) demineralizing bone tissue to provide a demineralized bone matrix from which at least a substantial portion of the original calcium content of the tissue has been removed, said matrix being made up of acid-insoluble collagenous residue and non-collagenous proteinaceous matter; b) subjecting the demineralized bone matrix to acid-promoted cleavage of intra-molecular bonds in collagen molecules and inter-molecular bonds between collagen molecules to provide a cleavage product which is acid-soluble upon heating; and c) heating the cleavage product to provide a liquid surface-adherent osteogenic composition.