US6696074B2
Expired / Lapsed
US
⏱️ 0.0 years remaining
Processing fetal or neo-natal tissue to produce a scaffold for tissue engineering
Assignee: TEI Biosciences Inc
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Filing: 2001-05-31
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Granted: 2004-02-24
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Expires: 2021-05-31
Abstract
Abstract A method of forming and preserving a bioremodelable, biopolymer scaffold material is carried out by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The process may include, but is not limited to, harvesting the tissue, optionally extracting growth and differentiation factors from the tissue, inactivating infective agents of the tissue such as by treating with KOH or NaOH, mechanically expressing undesirable components from the tissue, delipidizing the tissue with organic solvents, mechanically expressing components released by the solvet, washing the tissue, optionally drying the tissue, and optionally cross-linking the tissue, not necessarily in the order described. The resulting product, named EB Matrix (EBM), is characterized by its microbial, fungal, viral and prion inactivated state. EBM is strong, bioremodelable, drapable and does not undergo calcification.
Inventors
- 👤 Jianwu Dai
- 👤 Eugene Bell
- 👤 Vladimir Russakovsky
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 07:54
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/3683; A61L27/3691; A61F; A61F2/00; A61F2/02; A61F2/24; A61F2/2412; A61F2/2415; A61K; A61K35/00; A61K35/12; A61K35/34; A61K35/44; A61L27/3604; A61L27/362; A61L27/3687; A61L27/38; A61L27/3804; A61L27/50; A61L27/507; C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; C12N5/0656; A61L2430/00; A61L2430/40; C12N2502/00; C12N2502/02; C12N2502/14; C12N2533/00; C12N2533/50; C12N2533/54
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
87 words
A method for producing a biopolymer scaffold, comprising: (a) providing fetal tissue from an animal; (b) treating the tissue with KOH or NaOH; (c) applying pressure to the tissue to release components disassociated from the tissue by treatment with KOH or NaOH; (d) rinsing the tissue in aqueous solution; (e) treating the tissue with organic solvents to delipidize the tissue; (f) applying pressure to the tissue to release components disassociated from the tissue by treatment with organic solvents; and (g) rinsing the tissue in an aqueous solution.
Claim 2
Independent Claim
87 words
A method for producing a biopolymer scaffold, comprising: (a) providing fetal tissue from an animal; (b) treating the tissue with KOH or NaOH; (c) applying pressure to the tissue to release components disassociated from the tissue by treatment with KOH or NaOH; (d) rinsing the tissue in aqueous solution; (e) treating the tissue with organic solvents to delipidize the tissue; (f) applying pressure to the tissue to release components disassociated from the tissue by treatment with organic solvents; and (g) rinsing the tissue in an aqueous solution.
Claim 3
Dependent Claim
13 words
The method of claim 1 wherein the fetal tissue is bovine fetal tissue.
Claim 4
Dependent Claim
22 words
The method of claim 2 wherein the bovine fetal tissue is from a source between 10 weeks of age and newborn age.
Claim 5
Dependent Claim
11 words
The method of claim 1 wherein the fetal tissue is skin.
Claim 6
Dependent Claim
19 words
The method of claim 4 further comprising removing subdermal and epidermal tissue prior to treatment with KOH or NaOH.
Claim 7
Dependent Claim
29 words
The method of claim 1 wherein treatment with KOH or NaOH comprises treatment with 0.1 N to 10 N KOH or NaOH for between 10 minutes and 2 hours.
Claim 8
Dependent Claim
20 words
The method of claim 1 wherein treatment with KOH or NaOH takes place at between −4° C. and 10° C.
Claim 9
Dependent Claim
31 words
The method of claim 1 wherein treatment with organic solvents comprises treatment with a solution comprising a solvent selected from the group consisting of: chloroform, acetone, ether, alcohols, and mixtures thereof.
Claim 10
Dependent Claim
19 words
The method of claim 8 wherein treatment with organic solvents comprises treatment with a mixture of chloroform and ethanol.
Claim 11
Dependent Claim
20 words
The method of claim 1 wherein the tissue is rinsed with an aqueous alcohol solution after treatment with organic solvents.
Claim 12
Dependent Claim
12 words
The method of claim 1 further comprising: (h) freeze drying the tissue.
Claim 13
Dependent Claim
12 words
The method of claim 1 wherein the tissue is not chemically cross-linked.
Claim 14
Dependent Claim
18 words
The method of claim 1 wherein the tissue is treated with genipin or DHT to cross-link the tissue.
Claim 15
Independent Claim
87 words
A method for producing a biopolymer scaffold, comprising: (a) providing neo-natal tissue from an animal; (b) treating the tissue with KOH or NaOH; (c) applying pressure to the tissue to release components disassociated from the tissue by treatment with KOH or NaOH; (d) rinsing the tissue in aqueous solution; (e) treating the tissue with organic solvents to delipidize the tissue; (f) applying pressure to the tissue to release components disassociated from the tissue by treatment with organic solvents; and (g) rinsing the tissue in an aqueous solution.
Claim 16
Independent Claim
87 words
A method for producing a biopolymer scaffold, comprising: (a) providing neo-natal tissue from an animal; (b) treating the tissue with KOH or NaOH; (c) applying pressure to the tissue to release components disassociated from the tissue by treatment with KOH or NaOH; (d) rinsing the tissue in aqueous solution; (e) treating the tissue with organic solvents to delipidize the tissue; (f) applying pressure to the tissue to release components disassociated from the tissue by treatment with organic solvents; and (g) rinsing the tissue in an aqueous solution.
Claim 17
Dependent Claim
12 words
The method of claim 14 wherein the neo-natal tissue is porcine tissue.
Claim 18
Dependent Claim
11 words
The method of claim 14 wherein the neo-natal tissue is skin.
Claim 19
Dependent Claim
19 words
The method of claim 16 further comprising removing subdermal and epidermal tissue prior to treatment with KOH or NaOH.
Claim 20
Dependent Claim
29 words
The method of claim 14 wherein treatment with KOH or NaOH comprises treatment with 0.1 N to 10 N KOH or NaOH for between 10 minutes and 2 hours.
Claim 21
Dependent Claim
20 words
The method of claim 14 wherein treatment with KOH or NaOH takes place at between −4° C. and 10° C.
Claim 22
Dependent Claim
31 words
The method of claim 14 wherein treatment with organic solvents comprises treatment with a solution comprising a solvent selected from the group consisting of: ethanol chloroform, acetone, ether, and mixtures thereof.
Claim 23
Dependent Claim
19 words
The method of claim 20 wherein treatment with organic solvents comprises treatment with a mixture of chloroform and ethanol.
Claim 24
Dependent Claim
20 words
The method of claim 14 wherein the tissue is rinsed with an aqueous alcohol solution after treatment with organic solvents.
Claim 25
Dependent Claim
12 words
The method of claim 14 further comprising: (h) freeze drying the tissue.
Claim 26
Dependent Claim
12 words
The method of claim 14 wherein the tissue is not chemically cross-linked.
Claim 27
Dependent Claim
18 words
The method of claim 14 wherein the tissue is treated with genipin or DHT to cross-linked the tissue.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2007-02-23 | 2007-08-25 | 2008-02-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2011-02-23 | 2011-08-25 | 2012-02-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2015-02-23 | 2015-08-25 | 2016-02-24 | $7700.00 | Window Closed / Lapsed |