US6335016B1
Expired / Lapsed
US
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Chicken embryo lethal orphan (CELO) virus
Assignee: Boehringer Ingelheim International GmbH
•
Filing: 1997-04-18
•
Granted: 2002-01-01
•
Expires: 2017-04-18
Abstract
Abstract A CELO virus obtained by in vitro manipulation of a plasmid-cloned CELO virus DNA is suitable for the production of vectors for gene therapy and as a vaccine against infectious diseases in humans and animals, particularly birds.
Inventors
- 👤 Adam Baker
- 👤 Matthew Cotten
- 👤 Susanna Chiocca
- 👤 Robert Kurzbauer
- 👤 Gotthold Schaffner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 22:28
CPC Classifications
C; C12; C12N; C12N7/00; A; A61; A61P; A61P31/00; A61P35/00; C07; C07K; C07K14/00; C07K14/005; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A61K; A61K39/00; A61K2039/51; A61K2039/525; C12N2710/00; C12N2710/00011; C12N2710/10011; C12N2710/10211; C12N2710/10222; C12N2710/10241; C12N2710/10243
Claims Analysis
Total Claims: 169
Claim 1
Independent Claim
81 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of all or part of, an insertion in, or a mutation in, one or more non-essential regions selected from the group consisting of: (a) nucleotides from about 794 to about 1,330 of SEQ ID NO:1; and (b) nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 2
Dependent Claim
81 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of all or part of, an insertion in, or a mutation in, one or more non-essential regions selected from the group consisting of: (a) nucleotides from about 794 to about 1,330 of SEQ ID NO:1; and (b) nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 3
Dependent Claim
27 words
The CELO virus DNA of claim 1 , wherein said deletion, insertion or mutation occurs within nucleotides from about 794 to about 1,330 of SEQ ID NO:1.
Claim 4
Dependent Claim
28 words
The CELO virus DNA of claim 2 , wherein said CELO virus DNA contains a deletion of nucleotides from about 794 to about 1,330 of SEQ ID NO:1.
Claim 5
Dependent Claim
76 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of part of, an insertion in, or a mutation in, a non-essential region consisting of nucleotides from about 31,800 to about 43,734 of SEQ ID NO:1, and wherein said CELO virus DNA yields CELO virus particles in suitable cells with or without complementation.
Claim 6
Dependent Claim
76 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of part of, an insertion in, or a mutation in, a non-essential region consisting of nucleotides from about 31,800 to about 43,734 of SEQ ID NO:1, and wherein said CELO virus DNA yields CELO virus particles in suitable cells with or without complementation.
Claim 7
Dependent Claim
32 words
The CELO virus DNA of claim 4 , wherein said CELO virus DNA further contains a deletion, insertion or mutation within nucleotides from about 794 to about 1,330 of SEQ ID NO:1.
Claim 8
Dependent Claim
27 words
The CELO virus DNA of claim 1 , wherein said deletion, insertion or mutation occurs within nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 9
Dependent Claim
28 words
The CELO virus DNA of claim 6 , wherein said CELO virus DNA contains a deletion of nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 10
Dependent Claim
28 words
The CELO virus DNA of claim 5 , wherein said CELO virus DNA contains a deletion of nucleotides from about 794 to about 1,330 of SEQ ID NO:1.
Claim 11
Dependent Claim
32 words
The CELO virus DNA of claim 4 , wherein said CELO virus DNA further contains a deletion, insertion or mutation within nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 12
Dependent Claim
35 words
The CELO virus DNA of claim 1 , wherein said deletion, insertion or mutation occurs within nucleotides from about 794 to about 1,330 and nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 13
Dependent Claim
36 words
The CELO virus DNA of claim 10 , wherein said CELO virus DNA contains a deletion of nucleotides from about 794 to about 1,330 and nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 14
Dependent Claim
28 words
The CELO virus DNA of claim 9 , wherein said CELO virus DNA contains a deletion of nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 15
Dependent Claim
39 words
The CELO virus DNA of claim 4 , wherein CELO virus DNA further contains a deletion, insertion or mutation within nucleotides from about 794 to about 1,330 and nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 16
Dependent Claim
36 words
The CELO virus DNA of claim 13 , wherein said CELO virus DNA contains a deletion of nucleotides from about 794 to about 1,330 and nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1.
Claim 17
Dependent Claim
22 words
The CELO virus DNA of claim 4 , wherein said CELO virus genome comprises nucleotides 1 to 43,804 in SEQ ID NO:1.
Claim 18
Dependent Claim
34 words
The CELO virus DNA of claim 4 , wherein said CELO virus DNA is contained on a plasmid which replicates in bacteria or yeast and which yields virus particles after being introduced into cells.
Claim 19
Dependent Claim
22 words
The CELO virus DNA of claim 1 , wherein said CELO virus genome comprises nucleotides 1 to 43,804 in SEQ ID NO:1.
Claim 20
Dependent Claim
34 words
The CELO virus DNA of claim 1 , wherein said CELO virus DNA is contained on a plasmid which replicates in bacteria or yeast and which yields virus particles after being introduced into cells.
Claim 21
Dependent Claim
53 words
The CELO virus DNA of claim 18 , wherein said CELO virus DNA contains a deletion of a gene which is necessary for the formation of mature virus particles, and wherein said CELO virus DNA, after being introduced into cells together with a plasmid which complements said deleted gene, yields mature virus particles.
Claim 22
Dependent Claim
16 words
The CELO virus DNA of claim 1 , wherein said CELO virus DNA contains foreign DNA.
Claim 23
Dependent Claim
15 words
The CELO virus DNA of claim 20 , wherein said foreign DNA encodes a protein.
Claim 24
Dependent Claim
18 words
The CELO virus DNA of claim 20 , wherein said foreign DNA is inserted in a deleted section.
Claim 25
Dependent Claim
16 words
The CELO virus DNA of claim 19 , wherein said CELO virus DNA contains foreign DNA.
Claim 26
Dependent Claim
15 words
The CELO virus DNA of claim 23 , wherein said foreign DNA encodes a protein.
Claim 27
Dependent Claim
18 words
The CELO virus DNA of claim 23 , wherein said foreign DNA is inserted in a deleted section.
Claim 28
Dependent Claim
18 words
The CELO virus DNA of claim 21 , wherein said foreign DNA codes for a therapeutically active protein.
Claim 29
Dependent Claim
17 words
The CELO virus DNA of claim 26 , wherein said foreign DNA codes for an immunostimulatory protein.
Claim 30
Dependent Claim
16 words
The CELO virus DNA of claim 27 , wherein said foreign DNA codes for a cytokine.
Claim 31
Dependent Claim
72 words
The CELO virus DNA of claim 26 , wherein said foreign DNA codes for a protein selected from the group consisting of IL-1, IL-2, IL-6, IL-12, GM-CSF, an interferon, IκB, a glucocorticoid receptor, a catalase, manganese superoxide dismutase, glutathione peroxidase, LIP, LAP, ADF, Bcl-2, adenovirus E1B19K, Mcl-2, BAX, IRF-2, ICE protease, cJun, TAM-67, adenovirus E1A, p53, factor VIII, factor IX, erythropoietin, cystic fibrosis transmembrane regulator, dystrophin, globin, the LDL receptor and β-glucuronidase.
Claim 32
Dependent Claim
22 words
The CELO virus DNA of claim 20 , wherein said foreign DNA codes for a tumor antigen or an immunogenic fragment thereof.
Claim 33
Dependent Claim
20 words
The CELO virus DNA of claim 20 , wherein said foreign DNA codes for an antigen from a human pathogen.
Claim 34
Dependent Claim
20 words
The CELO virus DNA of claim 20 , wherein said foreign DNA codes for an antigen from an animal pathogen.
Claim 35
Dependent Claim
20 words
The CELO virus DNA of claim 32 , wherein said foreign DNA codes for an antigen from a bird pathogen.
Claim 36
Dependent Claim
19 words
The CELO virus DNA of claim 20 , wherein said foreign DNA codes for a ligand for mammalian cells.
Claim 37
Dependent Claim
53 words
The CELO virus DNA of claim 16 , wherein said CELO virus DNA contains a deletion of a gene which is necessary for the formation of mature virus particles, and wherein said CELO virus DNA, after being introduced into cells together with a plasmid which complements said deleted gene, yields mature virus particles.
Claim 38
Dependent Claim
38 words
The CELO virus DNA of claim 20 , wherein said CELO virus DNA contains said foreign DNA on a section comprising nucleotides from about 28,114 to about 30,495 of SEQ ID NO:1, which contains the fibre 1 gene.
Claim 39
Dependent Claim
35 words
The CELO virus DNA of claim 20 , wherein said CELO virus DNA contains said foreign DNA in the region of the reading frame at nucleotide 794 of SEQ ID NO:1 which codes for dUTPase.
Claim 40
Dependent Claim
48 words
The CELO virus DNA of claim 1 , wherein said left terminal repeat comprises nucleotides 1 to 68 in SEQ ID NO:1, said packaging signal comprises nucleotides 70 to 200 in SEQ ID NO:1, and said right terminal repeat comprises nucleotides 43,734 to 43,804 in SEQ ID NO:1.
Claim 41
Dependent Claim
19 words
The CELO virus DNA of claim 20 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 42
Dependent Claim
19 words
The CELO virus DNA of claim 23 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 43
Dependent Claim
22 words
The CELO virus DNA of claim 39 , wherein said regulatory sequence is selected from the group consisting of promoters and enhancers.
Claim 44
Dependent Claim
40 words
The CELO virus DNA of claim 41 , wherein said promoter is selected from the group consisting of the CMV immediate early promoter, the Rous Sarcoma Virus LTR, the adenovirus major late promoter and the CELO virus major late promoter.
Claim 45
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 1 .
Claim 46
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 20 .
Claim 47
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 23 .
Claim 48
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 39 .
Claim 49
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 40 .
Claim 50
Dependent Claim
26 words
A method for producing a protein comprising culturing the host cell of claim 44 under conditions such that said protein is expressed, and recovering said protein.
Claim 51
Dependent Claim
26 words
A method for producing a protein comprising culturing the host cell of claim 45 under conditions such that said protein is expressed, and recovering said protein.
Claim 52
Dependent Claim
65 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of nucleotides within the region from about 201 to about 5,000 of SEQ ID NO:1, and wherein said CELO virus DNA yields CELO virus particles in suitable cells with or without complementation.
Claim 53
Dependent Claim
65 words
A fowl adenovirus type 1 (CELO) virus DNA comprising the left and right inverted terminal repeats and the packaging signal of the CELO virus genome, wherein said CELO virus DNA contains a deletion of nucleotides within the region from about 201 to about 5,000 of SEQ ID NO:1, and wherein said CELO virus DNA yields CELO virus particles in suitable cells with or without complementation.
Claim 54
Dependent Claim
22 words
The CELO virus DNA of claim 50 , wherein said CELO virus genome comprises nucleotides 1 to 43,804 in SEQ ID NO:1.
Claim 55
Dependent Claim
34 words
The CELO virus DNA of claim 50 , wherein said CELO virus DNA is contained on a plasmid which replicates in bacteria or yeast and which yields virus particles after being introduced into cells.
Claim 56
Dependent Claim
53 words
The CELO virus DNA of claim 52 , wherein said CELO virus DNA contains a deletion of a gene which is necessary for the formation of mature virus particles, and wherein said CELO virus DNA, after being introduced into cells together with a plasmid which complements said deleted gene, yields mature virus particles.
Claim 57
Dependent Claim
16 words
The CELO virus DNA of claim 50 , wherein said CELO virus DNA contains foreign DNA.
Claim 58
Dependent Claim
15 words
The CELO virus DNA of claim 54 , wherein said foreign DNA encodes a protein.
Claim 59
Dependent Claim
18 words
The CELO virus DNA of claim 54 , wherein said foreign DNA is inserted in the deleted section.
Claim 60
Dependent Claim
16 words
The CELO virus DNA of claim 53 , wherein said CELO virus DNA contains foreign DNA.
Claim 61
Dependent Claim
15 words
The CELO virus DNA of claim 57 , wherein said foreign DNA encodes a protein.
Claim 62
Dependent Claim
18 words
The CELO virus DNA of claim 57 , wherein said foreign DNA is inserted in the deleted section.
Claim 63
Dependent Claim
18 words
The CELO virus DNA of claim 55 , wherein said foreign DNA codes for a therapeutically active protein.
Claim 64
Dependent Claim
17 words
The CELO virus DNA of claim 60 , wherein said foreign DNA codes for an immunostimulatory protein.
Claim 65
Dependent Claim
16 words
The CELO virus DNA of claim 61 , wherein said foreign DNA codes for a cytokine.
Claim 66
Dependent Claim
72 words
The CELO virus DNA of claim 60 , wherein said foreign DNA codes for a protein selected from the group consisting of IL-1, IL-2, IL-6, IL-12, GM-CSF, an interferon, IκB, a glucocorticoid receptor, a catalase, manganese superoxide dismutase, glutathione peroxidase, LIP, LAP, ADF, Bcl-2, adenovirus E1B19K, Mcl-2, BAX, IRF-2, ICE protease, cjun, TAM-67, adenovirus E1A, p53, factor VIII, factor IX, erythropoietin, cystic fibrosis transmembrane regulator, dystrophin, globin, the LDL receptor and β-glucuronidase.
Claim 67
Dependent Claim
22 words
The CELO virus DNA of claim 54 , wherein said foreign DNA codes for a tumor antigen or an immunogenic fragment thereof.
Claim 68
Dependent Claim
20 words
The CELO virus DNA of claim 54 , wherein said foreign DNA codes for an antigen from a human pathogen.
Claim 69
Dependent Claim
20 words
The CELO virus DNA of claim 54 , wherein said foreign DNA codes for an antigen from an animal pathogen.
Claim 70
Dependent Claim
20 words
The CELO virus DNA of claim 66 , wherein said foreign DNA codes for an antigen from a bird pathogen.
Claim 71
Dependent Claim
19 words
The CELO virus DNA of claim 54 , wherein said foreign DNA codes for a ligand for mammalian cells.
Claim 72
Dependent Claim
48 words
The CELO virus DNA of claim 50 , wherein said left terminal repeat comprises nucleotides 1 to 68 in SEQ ID NO:1, said packaging signal comprises nucleotides 70 to 200 in SEQ ID NO:1, and said right terminal repeat comprises nucleotides 43,734 to 43,804 in SEQ ID NO:1.
Claim 73
Dependent Claim
19 words
The CELO virus DNA of claim 54 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 74
Dependent Claim
19 words
The CELO virus DNA of claim 57 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 75
Dependent Claim
22 words
The CELO virus DNA of claim 70 , wherein said regulatory sequence is selected from the group consisting of promoters and enhancers.
Claim 76
Dependent Claim
40 words
The CELO virus DNA of claim 72 , wherein said promoter is selected from the group consisting of the CMV immediate early promoter, the Rous Sarcoma Virus LTR, the adenovirus major late promoter and the CELO virus major late promoter.
Claim 77
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 50 .
Claim 78
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 54 .
Claim 79
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 57 .
Claim 80
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 70 .
Claim 81
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 71 .
Claim 82
Dependent Claim
27 words
A method for producing of a protein comprising culturing the host cell of claim 54 under conditions such that said protein is expressed, and recovering said protein.
Claim 83
Dependent Claim
27 words
A method for producing of a protein comprising culturing the host cell of claim 57 under conditions such that said protein is expressed, and recovering said protein.
Claim 84
Dependent Claim
31 words
Process for preparing recombinant CELO virus DNA according to claim 1 , characterized in that the CELO virus genome or sections thereof are contained on a plasmid and are genetically manipulated.
Claim 85
Dependent Claim
41 words
Process according to claim 81 , characterized in that the CELO virus genome or sections thereof contained on a plasmid are manipulated in a region which is different from the left and right inverted terminal repeat and from the packaging signal.
Claim 86
Dependent Claim
18 words
Process according to claim 81 , characterized in that insertions, insertions and deletions, or deletions are carried out.
Claim 87
Dependent Claim
18 words
Process according to claim 82 , characterized in that insertions, insertions and deletions, or deletions are carried out.
Claim 88
Dependent Claim
55 words
Process according to claim 83 , characterized in that a foreign gene is inserted in a naturally occurring or artificially introduced restriction cutting site on a section of the CELO virus DNA which contains a sequence which is not necessary for the replication of the virus in the host cell or which can be complemented.
Claim 89
Dependent Claim
50 words
Process according to claim 85 , characterized in that in addition to the insertion, a deletion is carried out in another region of the CELO virus DNA which contains a sequence which is not necessary for the replication of the virus in the host cell or which can be complemented.
Claim 90
Dependent Claim
16 words
Process according to claim 81 , characterized in that the manipulation is carried out by recombination.
Claim 91
Dependent Claim
16 words
Process according to claim 82 , characterized in that the manipulation is carried out by recombination.
Claim 92
Dependent Claim
18 words
Process according to claim 87 , characterized in that DNA molecules obtained by polymerase chain reaction are recombined.
Claim 93
Dependent Claim
16 words
Process according to claim 87 , characterized in that DNA molecules obtained by ligation are recombined.
Claim 94
Dependent Claim
18 words
Process according to claim 87 , characterized in that DNA molecules obtained by cloning in bacteria are recombined.
Claim 95
Dependent Claim
121 words
Process for preparing recombinant CELO virus DNA according to claim 91 , comprising: (a) cloning a CELO virus DNA fragment containing two restriction sites into a bacterial plasmid; (b) inserting foreign DNA between these restriction sites which occur only once on the plasmid; (c) excising the fragment containing the foreign DNA from the plasmid and mixing said fragment with a plasmid which contains the complete CELO virus DNA and which has been cut at a restriction cutting site which occurs only once; (d) transforming the bacteria with this mixture of DNA molecules and growing the bacteria; and (e) obtaining a plasmid by recombination of the DNA molecules which contains the entire CELO virus DNA with the foreign DNA as an insert.
Claim 96
Dependent Claim
18 words
Process according to claim 85 , characterized in that a reporter gene is inserted as the foreign DNA.
Claim 97
Dependent Claim
18 words
Process according to claim 87 , characterized in that a reporter gene is inserted as the foreign DNA.
Claim 98
Dependent Claim
34 words
Process according to claim 93 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a therapeutically active protein is inserted in an additional step.
Claim 99
Dependent Claim
37 words
Process according to claim 93 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a tumor antigen or a fragment thereof is inserted in an additional step.
Claim 100
Dependent Claim
37 words
Process according to claim 93 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from a human pathogen is inserted in an additional step.
Claim 101
Dependent Claim
37 words
Process according to claim 93 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from an animal pathogen is inserted in an additional step.
Claim 102
Dependent Claim
35 words
Process according to claim 93 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a ligand for mammalian cells is inserted in an additional step.
Claim 103
Dependent Claim
34 words
Process according to claim 94 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a therapeutically active protein is inserted in an additional step.
Claim 104
Dependent Claim
37 words
Process according to claim 94 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a tumor antigen or a fragment thereof is inserted in an additional step.
Claim 105
Dependent Claim
37 words
Process according to claim 94 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from a human pathogen is inserted in an additional step.
Claim 106
Dependent Claim
37 words
Process according to claim 94 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from an animal pathogen is inserted in an additional step.
Claim 107
Dependent Claim
35 words
Process according to claim 94 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a ligand for mammalian cell is inserted in an additional step.
Claim 108
Dependent Claim
48 words
Process for preparing CELO virus which comprises: (a) transforming avian cells with a plasmid containing CELO virus DNA of claim 1 , thereby obtaining transformed cells; (b) cultivating said transformed cells (a), thereby obtaining a cell culture; and (c) harvesting said cell culture (b), thereby preparing CELO virus.
Claim 109
Dependent Claim
33 words
Process according to claim 105 , characterized in that the CELO virus DNA lacks sequences which are necessary for the formation of mature virus particles, the process which further comprises complementing said sequences.
Claim 110
Dependent Claim
30 words
Process according to claim 106 , characterized in that the avian cells used are helper cells which complement said sequences which are necessary for the formation of mature virus particles.
Claim 111
Dependent Claim
30 words
Process according to claim 106 , characterized in that the avian cells are also transformed with a plasmid which complements said sequences necessary for the formation of mature virus particles.
Claim 112
Dependent Claim
31 words
Process according to claim 106 , characterized in that the avian cells are also infected with a helper virus which complements said sequences necessary for the formation of mature virus particles.
Claim 113
Dependent Claim
16 words
The CELO virus DNA of claim 4 , wherein said CELO virus DNA contains foreign DNA.
Claim 114
Dependent Claim
15 words
The CELO virus DNA of claim 110 , wherein said foreign DNA encodes a protein.
Claim 115
Dependent Claim
18 words
The CELO virus DNA of claim 110 , wherein said foreign DNA is inserted in a deleted section.
Claim 116
Dependent Claim
16 words
The CELO virus DNA of claim 35 , wherein said CELO virus DNA contains foreign DNA.
Claim 117
Dependent Claim
15 words
The CELO virus DNA of claim 113 , wherein said foreign DNA encodes a protein.
Claim 118
Dependent Claim
18 words
The CELO virus DNA of claim 113 , wherein said foreign DNA is inserted in a deleted section.
Claim 119
Dependent Claim
18 words
The CELO virus DNA of claim 111 , wherein said foreign DNA codes for a therapeutically active protein.
Claim 120
Dependent Claim
17 words
The CELO virus DNA of claim 116 , wherein said foreign DNA codes for an immunostimulatory protein.
Claim 121
Dependent Claim
16 words
The CELO virus DNA of claim 117 , wherein said foreign DNA codes for a cytokine.
Claim 122
Dependent Claim
72 words
The CELO virus DNA of claim 116 , wherein said foreign DNA codes for a protein selected from the group consisting of IL-1, IL-2, IL-6, IL-12, GM-CSF, an interferon, IκB, a glucocorticoid receptor, a catalase, manganese superoxide dismutase, glutathione peroxidase, LIP, LAP, ADF, Bcl-2, adenovirus E1B19K, Mcl-2, BAX, IRF-2, ICE protease, cjun, TAM-67, adenovirus E1A, p53, factor VIII, factor IX, erythropoietin, cystic fibrosis transmembrane regulator, dystrophin, globin, the LDL receptor and β-glucuronidase.
Claim 123
Dependent Claim
22 words
The CELO virus DNA of claim 110 , wherein said foreign DNA codes for a tumor antigen or an immunogenic fragment thereof.
Claim 124
Dependent Claim
20 words
The CELO virus DNA of claim 110 , wherein said foreign DNA codes for an antigen from a human pathogen.
Claim 125
Dependent Claim
20 words
The CELO virus DNA of claim 110 , wherein said foreign DNA codes for an antigen from an animal pathogen.
Claim 126
Dependent Claim
20 words
The CELO virus DNA of claim 122 , wherein said foreign DNA codes for an antigen from a bird pathogen.
Claim 127
Dependent Claim
19 words
The CELO virus DNA of claim 110 , wherein said foreign DNA codes for a ligand for mammalian cells.
Claim 128
Dependent Claim
33 words
The CELO virus DNA of claim 110 , wherein said CELO virus DNA contains said foreign DNA at about the FseI cutting site which is located at position 35,693 of SEQ ID NO:1.
Claim 129
Dependent Claim
48 words
The CELO virus DNA of claim 4 , wherein said left terminal repeat comprises nucleotides 1 to 68 in SEQ ID NO:1, said packaging signal comprises nucleotides 70 to 200 in SEQ ID NO:1, and said right terminal repeat comprises nucleotides 43,734 to 43,804 in SEQ ID NO:1.
Claim 130
Dependent Claim
19 words
The CELO virus DNA of claim 110 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 131
Dependent Claim
19 words
The CELO virus DNA of claim 113 , wherein said foreign DNA is operably associated with a regulatory sequence.
Claim 132
Dependent Claim
22 words
The CELO virus DNA of claim 127 , wherein said regulatory sequence is selected from the group consisting of promoters and enhancers.
Claim 133
Dependent Claim
40 words
The CELO virus DNA of claim 129 , wherein said promoter is selected from the group consisting of the CMV immediate early promoter, the Rous Sarcoma Virus LTR, the adenovirus major late promoter and the CELO virus major late promoter.
Claim 134
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 4 .
Claim 135
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 110 .
Claim 136
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 113 .
Claim 137
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 127 .
Claim 138
Dependent Claim
12 words
A host cell comprising the CELO virus DNA of claim 128 .
Claim 139
Dependent Claim
26 words
A method for producing a protein comprising culturing the host cell of claim 132 under conditions such that said protein is expressed, and recovering said protein.
Claim 140
Dependent Claim
26 words
A method for producing a protein comprising culturing the host cell of claim 133 under conditions such that said protein is expressed, and recovering said protein.
Claim 141
Dependent Claim
32 words
A process for preparing recombinant CELO virus DNA according to claim 4 , characterized in that the CELO virus genome or sections thereof are contained on a plasmid and are genetically manipulated.
Claim 142
Dependent Claim
42 words
The process according to claim 138 , characterized in that the CELO virus genome or sections thereof contained on a plasmid are manipulated in a region which is different from the left and right inverted terminal repeat and from the packaging signal.
Claim 143
Dependent Claim
19 words
The process according to claim 138 , characterized in that insertions, insertions and deletions, or deletions are carried out.
Claim 144
Dependent Claim
19 words
The process according to claim 139 , characterized in that insertions, insertions and deletions, or deletions are carried out.
Claim 145
Dependent Claim
56 words
The process according to claim 140 , characterized in that a foreign gene is inserted in a naturally occurring or artificially introduced restriction cutting site on a section of the CELO virus DNA which contains a sequence which is not necessary for the replication of the virus in the host cell or which can be complemented.
Claim 146
Dependent Claim
51 words
The process according to claim 142 , characterized in that in addition to the insertion, a deletion is carried out in another region of the CELO virus DNA which contains a sequence which is not necessary for the replication of the virus in the host cell or which can be complemented.
Claim 147
Dependent Claim
17 words
The process according to claim 138 , characterized in that the manipulation is carried out by recombination.
Claim 148
Dependent Claim
17 words
The process according to claim 139 , characterized in that the manipulation is carried out by recombination.
Claim 149
Dependent Claim
19 words
The process according to claim 144 , characterized in that DNA molecules obtained by polymerase chain reaction are recombined.
Claim 150
Dependent Claim
17 words
The process according to claim 144 , characterized in that DNA molecules obtained by ligation are recombined.
Claim 151
Dependent Claim
19 words
The process according to claim 144 , characterized in that DNA molecules obtained by cloning in bacteria are recombined.
Claim 152
Dependent Claim
122 words
The process for preparing recombinant CELO virus DNA according to claim 148 , comprising: (a) cloning a CELO virus DNA fragment containing two restriction sites into a bacterial plasmid; (b) inserting foreign DNA between these restriction sites which occur only once on the plasmid; (c) excising the fragment containing the foreign DNA from the plasmid and mixing said fragment with a plasmid which contains the complete CELO virus DNA and which has been cut at a restriction cutting site which occurs only once; (d) transforming the bacteria with this mixture of DNA molecules and growing the bacteria; and (e) obtaining a plasmid by recombination of the DNA molecules which contains the entire CELO virus DNA with the foreign DNA as an insert.
Claim 153
Dependent Claim
19 words
The process according to claim 142 , characterized in that a reporter gene is inserted as the foreign DNA.
Claim 154
Dependent Claim
19 words
The process according to claim 144 , characterized in that a reporter gene is inserted as the foreign DNA.
Claim 155
Dependent Claim
35 words
The process according to claim 150 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a therapeutically active protein is inserted in an additional step.
Claim 156
Dependent Claim
38 words
The process according to claim 150 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a tumor antigen or a fragment thereof is inserted in an additional step.
Claim 157
Dependent Claim
38 words
The process according to claim 150 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from a human pathogen is inserted in an additional step.
Claim 158
Dependent Claim
38 words
The process according to claim 150 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from an animal pathogen is inserted in an additional step.
Claim 159
Dependent Claim
36 words
The process according to claim 150 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a ligand for mammalian cells is inserted in an additional step.
Claim 160
Dependent Claim
35 words
The process according to claim 151 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a therapeutically active protein is inserted in an additional step.
Claim 161
Dependent Claim
38 words
The process according to claim 151 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a tumor antigen or a fragment thereof is inserted in an additional step.
Claim 162
Dependent Claim
38 words
The process according to claim 151 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from a human pathogen is inserted in an additional step.
Claim 163
Dependent Claim
38 words
The process according to claim 151 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for an antigen derived from an animal pathogen is inserted in an additional step.
Claim 164
Dependent Claim
36 words
The process according to claim 151 , characterized in that the reporter gene has a restriction cutting site into which foreign DNA which codes for a ligand for mammalian cells is inserted in an additional step.
Claim 165
Dependent Claim
49 words
A process for preparing CELO virus which comprises: (a) transforming avian cells with a plasmid containing CELO virus DNA of claim 4 , thereby obtaining transformed cells; (b) cultivating said transformed cells (a), thereby obtaining a cell culture; and (c) harvesting said cell culture (b), thereby preparing CELO virus.
Claim 166
Dependent Claim
30 words
The process according to claim 162 , characterized in that the CELO virus DNA lacks sequences which are necessary for the formation of mature virus particles and complementing said sequences.
Claim 167
Dependent Claim
31 words
The process according to claim 163 , characterized in that the avian cells used are helper cells which complement said sequences which are necessary for the formation of mature virus particles.
Claim 168
Dependent Claim
31 words
The process according to claim 163 , characterized in that the avian cells are also transformed with a plasmid which complements said sequences necessary for the formation of mature virus particles.
Claim 169
Dependent Claim
32 words
The process according to claim 163 , characterized in that the avian cells are also infected with a helper virus which complements said sequences necessary for the formation of mature virus particles.
References Cited (Backward Citations)
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Cited
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Cited
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Cited
WO1996003517A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2004-12-31 | 2005-07-02 | 2006-01-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2008-12-31 | 2009-07-02 | 2010-01-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2012-12-31 | 2013-07-02 | 2014-01-01 | $7700.00 | Window Closed / Lapsed |