US6068829A
Expired / Lapsed
US
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Method of identifying molecules that home to a selected organ in vivo
Assignee: Sanford Burnham Prebys Medical Discovery Institute
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Filing: 1997-05-23
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Granted: 2000-05-30
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Expires: 2017-05-23
Abstract
Abstract The present invention provides methods for in vivo panning of a library to identify molecules that specifically home to a selected organ.
Inventors
- 👤 Erkki Ruoslahti
- 👤 Renata Pasqualini
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 08:37
CPC Classifications
A; A61; A61K; A61K49/00; A61K49/0004; A61K49/0008; C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6883
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
93 words
A method of recovering a plurality of peptide or peptidomimetic molecules that home to a selected organ or tissue, comprising the steps of: a. administering to a subject a library of diverse peptide or peptidomimetic molecules, wherein each of said diverse molecules is linked to a tag that facilitates recovery of said peptide or peptidomimetic molecules; b. collecting a sample of the selected organ or tissue; and c. recovering a plurality of peptide or peptidomimetic molecules that home to said selected organ or tissue by isolating molecules comprising said tag from said sample.
Claim 2
Independent Claim
93 words
A method of recovering a plurality of peptide or peptidomimetic molecules that home to a selected organ or tissue, comprising the steps of: a. administering to a subject a library of diverse peptide or peptidomimetic molecules, wherein each of said diverse molecules is linked to a tag that facilitates recovery of said peptide or peptidomimetic molecules; b. collecting a sample of the selected organ or tissue; and c. recovering a plurality of peptide or peptidomimetic molecules that home to said selected organ or tissue by isolating molecules comprising said tag from said sample.
Claim 3
Dependent Claim
11 words
The method of claim 1, wherein said tag is a support.
Claim 4
Dependent Claim
21 words
The method of claim 1, wherein said selected organ or tissue is selected from the group consisting of brain and kidney.
Claim 5
Dependent Claim
14 words
The method of claim 1, wherein said selected organ or tissue is a tumor.
Claim 6
Dependent Claim
15 words
The method of claim 1, wherein said library is a library of diverse peptide molecules.
Claim 7
Dependent Claim
17 words
The method of claim 1, wherein said library comprises at least ten diverse peptide or peptidomimetic molecules.
Claim 8
Dependent Claim
18 words
The method of claim 1, wherein said library comprises several billion or more diverse peptide or peptidomimetic molecules.
Claim 9
Dependent Claim
13 words
The method of claim 1, wherein said tag that facilitates recovery is biotin.
Claim 10
Dependent Claim
14 words
The method of claim 1, wherein said tag that facilitates recovery is a hapten.
Claim 11
Dependent Claim
15 words
The method of claim 1, wherein said tag that facilitates recovery is a virus particle.
Claim 12
Dependent Claim
15 words
The method of claim 1, wherein said tag that facilitates recovery is a eukaryotic cell.
Claim 13
Dependent Claim
14 words
The method of claim 1, wherein said tag that facilitates recovery is a liposome.
Claim 14
Dependent Claim
14 words
The method of claim 1, wherein said tag that facilitates recovery is a microbead.
Claim 15
Independent Claim
86 words
A method of identifying a peptide or peptidomimetic molecule that homes to a selected organ or tissue, comprising the steps of: a. administering to a subject a library of diverse peptide or peptidomimetic molecules, wherein each of said diverse peptide or peptidomimetic molecules is linked to a unique oligonucleotide tag; b. collecting a sample of the selected organ or tissue; and c. identifying a unique oligonucleotide tag present in said sample, thereby identifying a peptide or peptidomimetic molecule that homes to said selected organ or tissue.
Claim 16
Independent Claim
86 words
A method of identifying a peptide or peptidomimetic molecule that homes to a selected organ or tissue, comprising the steps of: a. administering to a subject a library of diverse peptide or peptidomimetic molecules, wherein each of said diverse peptide or peptidomimetic molecules is linked to a unique oligonucleotide tag; b. collecting a sample of the selected organ or tissue; and c. identifying a unique oligonucleotide tag present in said sample, thereby identifying a peptide or peptidomimetic molecule that homes to said selected organ or tissue.
Claim 17
Dependent Claim
31 words
The method of claim 14, wherein each of said diverse peptide or peptidomimetic molecules of said library is linked to a tag that facilitates recovery of a peptide or peptidomimetic molecule.
Claim 18
Dependent Claim
26 words
The method of claim 14, wherein each of said diverse peptide or peptidomimetic molecules of said library is linked to a tag that is a support.
Claim 19
Dependent Claim
21 words
the method of claim 14, wherein said selected organ or tissue is selected from the group consisting of brain and kidney.
Claim 20
Dependent Claim
14 words
The method of claim 14, wherein said selected organ or tissue is a tumor.
Claim 21
Dependent Claim
15 words
The method of claim 14, wherein said library is a library of diverse peptide molecules.
Claim 22
Dependent Claim
17 words
The method of claim 14, wherein said library comprises at least ten diverse peptide or peptidomimetic molecules.
Claim 23
Dependent Claim
18 words
The method of claim 14, wherein said library comprises several billion or more diverse peptide or peptidomimetic molecules.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 2003-05-30 | 2003-11-29 | 2004-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-05-30 | 2007-11-29 | 2008-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-05-30 | 2011-11-29 | 2012-05-30 | $7700.00 | Window Closed / Lapsed |