US5840502A
Expired / Lapsed
US
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Methods for enriching specific cell-types by density gradient centrifugation
Assignee: Dendreon Pharmaceuticals LLC
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Filing: 1994-08-31
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Granted: 1998-11-24
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Expires: 2014-08-31
Abstract
Abstract The present invention relates to methods of enriching for desired cell population from cell sources, such as body fluids, dispersed tissue specimens and cultured cells. In particular, the present invention relates to the use of a cell-trap centrifugation tube containing a specific density gradient solution adjusted to the specific density of a desired cell population to enrich for the desired cell from a cell source. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to the undesired populations in a more convenient manner. The rapid cell enrichment method described herein has a wide range of diagnostic and therapeutic applications.
Inventors
- 👤 Peter Van Vlasselaer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/483; G01N33/487; G01N33/49; G01N33/491; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; B01L3/508; B01L3/5082; C; C12; C12N; C12N5/00; C12N5/0081; C12N5/0093; G01N15/00; G01N15/10; G01N15/14; G01N15/1456; G01N15/1459; A; A61; A61M; A61M2202/00; A61M2202/04; A61M2202/0413; A61M2202/0439; G01N2015/1006; G01N2015/1402; G01N2015/1477; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S436/00; Y10S436/824
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
110 words
A centrifugation tube which, prior to centrifugation of said tube, comprises: a tube adapted for centrifugation having a first closed end defining an inner bottom wall and an opposite open end; an annular member disposed in said tube and defining an opening therethrough, wherein said opening has an area less than the area of a cross section of said tube, said annular member defining a lower portion of the tube extending between said member and the tube bottom wall and an upper portion above said annular member; and a density gradient solution filling said lower portion and a part of said upper portion to a level above said annular member.
Claim 2
Dependent Claim
110 words
A centrifugation tube which, prior to centrifugation of said tube, comprises: a tube adapted for centrifugation having a first closed end defining an inner bottom wall and an opposite open end; an annular member disposed in said tube and defining an opening therethrough, wherein said opening has an area less than the area of a cross section of said tube, said annular member defining a lower portion of the tube extending between said member and the tube bottom wall and an upper portion above said annular member; and a density gradient solution filling said lower portion and a part of said upper portion to a level above said annular member.
Claim 3
Dependent Claim
26 words
The tube of claim 1, wherein said density gradient solution fills the upper portion to a level at least about 1 mm above said annular member.
Claim 4
Dependent Claim
15 words
The tube of claim 1, wherein said annular member is formed integrally with said tube.
Claim 5
Dependent Claim
25 words
The tube of claim 1, wherein said annular member is slideably disposed within said tube to permit adjustment of the volume of the lower portion.
Claim 6
Dependent Claim
14 words
The tube of claim 1 wherein said annular member defines a plurality of openings.
Claim 7
Dependent Claim
32 words
The tube of claim 1 wherein said density gradient solution has an osmolality of 280±10 mOsm kg/H 2 O and a specific density selected from the range 1.0300 gr/ml to 1.2000 gr/ml.
Claim 8
Independent Claim
206 words
A method of enriching for a population of desired cells from a cell mixture containing multiple cell populations, comprising: layering a cell mixture containing the desired cells onto a gradient density solution contained in a centrifuge tube; said tube having a first closed end defining an inner bottom wall and an opposite open end; an annular member disposed in said tube and defining an opening therethrough, wherein said opening has an area less than the area of a cross section of said tube, said annular member defining a lower portion of the tube extending between said member and said tube bottom wall and upper portion above said annular member; said tube containing a density gradient solution which fills said lower portion and a part of said top portion to a level above said annular member; said density gradient solution having an osmolality of 280±10 mOsm/kg H 2 O and a specific density within 0.0005 gr/ml of the specific density of the desired cell; centrifuging said tube at a gravitational force sufficient to pellet cells having specific densities greater than the specific density of the density gradient material in said tube; and collecting from upper portion of said tube an enriched fraction of said population of cells.
Claim 9
Independent Claim
206 words
A method of enriching for a population of desired cells from a cell mixture containing multiple cell populations, comprising: layering a cell mixture containing the desired cells onto a gradient density solution contained in a centrifuge tube; said tube having a first closed end defining an inner bottom wall and an opposite open end; an annular member disposed in said tube and defining an opening therethrough, wherein said opening has an area less than the area of a cross section of said tube, said annular member defining a lower portion of the tube extending between said member and said tube bottom wall and upper portion above said annular member; said tube containing a density gradient solution which fills said lower portion and a part of said top portion to a level above said annular member; said density gradient solution having an osmolality of 280±10 mOsm/kg H 2 O and a specific density within 0.0005 gr/ml of the specific density of the desired cell; centrifuging said tube at a gravitational force sufficient to pellet cells having specific densities greater than the specific density of the density gradient material in said tube; and collecting from upper portion of said tube an enriched fraction of said population of cells.
Claim 10
Dependent Claim
21 words
The method of claim 7 wherein the specific density is within 0.0002 gr/ml of the specific density of said desired cells.
Claim 11
Dependent Claim
45 words
The method of claim 7 further comprising incubating said cell mixture with a cell type-specific binding agent linked to carrier particles prior to centrifugation, said particles having a specific density that is at least 0.001 gr/ml greater than the specific density of the desired cells.
Claim 12
Dependent Claim
14 words
The method of claim 9 wherein the cell type-specific binding agent is an antibody.
Claim 13
Dependent Claim
14 words
The method of claim 9 wherein the cell type-specific binding agent is a lectin.
Claim 14
Dependent Claim
14 words
The method of claim 9 wherein the cell type-specific binding agent is a cytokine.
Claim 15
Dependent Claim
13 words
The method of claim 9 wherein the carrier particle is a silica bead.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2001-11-23 | 2002-05-25 | 2002-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2005-11-23 | 2006-05-25 | 2006-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2009-11-23 | 2010-05-25 | 2010-11-24 | $7700.00 | Window Closed / Lapsed |