Patent US7151157B2 successfully refreshed from Google Patents.
US5045201A
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US
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Glass microbeads for biochemical separation of material from a fluid medium
Assignee: AGC Glass Europe SA
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Filing: 1989-09-18
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Granted: 1991-09-03
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Expires: 2009-09-18
Abstract
Abstract Glass microbeads bearing a coating which includes at least one binding agent fixed to the glass microbeads, and which binding agent is adapted releasably to bind to a material contained within a fluid medium by a biological affinity reaction, whereby the material can be removed from the fluid medium with the glass microbeads and then stripped from the glass microbeads while leaving the at least one binding agent attached to the glass microbeads. Inventive microbeads may bear a monomolecular layer of a silane as a fixing agent for a binding agent which is selected for its biological affinity for the material to be separated.
Inventors
- 👤 Dominique Dubois
- 👤 Marcel Deizant
- 👤 Francois Toussaint
- 👤 Thierry Kemp
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:21
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/10; B01D15/18; B01D15/1807; B01D15/26; B01D15/38; B01D15/3804; B01D15/3809; B01J; B01J20/00; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28011; B01J20/28014; B01J20/28016; B01J20/28019; B01J20/28021; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3263; B01J20/3268; B01J20/3272; B01J20/3274; B82; B82Y; B82Y30/00; B82Y5/00; C; C12; C12M; C12M47/00; C12M47/02; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2995; Y10T428/2996
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
111 words
Glass microbeads, comprising: glass microbeads which are glass microspheres having non-porous surfaces and which bear a coating comprised of at least one binding agent and at least one fixing agent which fixes the at least one binding agent to the non-porous surfaces of the glass microspheres by covalent bonding, and which at least one binding agent is capable of releasably binding, by a biological affinity reaction, a material contained within a fluid medium when in contact therewith, whereby the material can be removed from the fluid medium with the glass microbeads and then stripped from the glass microbeads while leaving the at least one binding agent attached to the glass microbeads.
Claim 2
Dependent Claim
111 words
Glass microbeads, comprising: glass microbeads which are glass microspheres having non-porous surfaces and which bear a coating comprised of at least one binding agent and at least one fixing agent which fixes the at least one binding agent to the non-porous surfaces of the glass microspheres by covalent bonding, and which at least one binding agent is capable of releasably binding, by a biological affinity reaction, a material contained within a fluid medium when in contact therewith, whereby the material can be removed from the fluid medium with the glass microbeads and then stripped from the glass microbeads while leaving the at least one binding agent attached to the glass microbeads.
Claim 3
Dependent Claim
15 words
The glass microbeads according to claim 1, wherein said glass microbeads are hollow glass microspheres.
Claim 4
Dependent Claim
25 words
The glass microbeads according to claim 2, wherein said glass microbeads have an average density ranging between 0.5 and 1.5 relative to the fluid medium.
Claim 5
Dependent Claim
25 words
The glass microbeads according to claim 3, wherein said glass microbeads have an average density ranging between 0.9 and 1.1 relative to the fluid medium.
Claim 6
Dependent Claim
18 words
The glass microbeads according to claim 1, wherein said glass microbeads have a median diameter below 125 μm.
Claim 7
Dependent Claim
25 words
The glass microbeads according to claim 5, wherein at least 90% of said glass microbeads have a diameter ranging between 20 μm and 30 μm.
Claim 8
Dependent Claim
14 words
The glass microbeads according to claim 1, wherein said fixing agent comprises a silane.
Claim 9
Dependent Claim
22 words
The glass microbeads according to claim 1, wherein said fixing agent is applied to said glass microbeads substantially as a monomolecular layer.
Claim 10
Dependent Claim
22 words
The glass microbeads according to claim 1, wherein said at least one binding agent is one of an antigen or an antibody.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-09-02 | 1995-03-04 | 1995-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-09-02 | 1999-03-04 | 1999-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-09-02 | 2003-03-04 | 2003-09-03 | $7700.00 | Window Closed / Lapsed |