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US5643789A
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Bioactive material template for in vitro synthesis of bone tissue
Assignee: University of Pennsylvania
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Filing: 1994-07-21
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Granted: 1997-07-01
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Expires: 2014-07-21
Abstract
Abstract Novel non-crystalline, porous bioactive glass and ceramic materials that permit the in vitro formation of bone tissue when exposed to a tissue culture medium and inoculated with cells are disclosed. The present invention also discloses methods of treating bioactive glass materials to control pH so that when the glass is exposed to a tissue culture medium and then inoculated with cells, bone tissue growth occurs in vitro. The glass material disclosed is preferably formed from SiO 2 , CaO, Na 2 O and P 2 O 5 and the porous, non-crystalline structure is most preferably created by melting the constituents, cooling and pulverizing the resulting glass, and then forming and hot pressing the powder. The glass of the present invention may be formed to produce templates that are useful for various indications, as well as granules that may be formed into a paste.
Inventors
- 👤 Paul Ducheyne
- 👤 Ahmed El-Ghannam
- 👤 Irving Shapiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:10
CPC Classifications
C; C03; C03C; C03C11/00; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0652; A; A61; A61F; A61F2/00; A61F2/02; A61F2/28; A61L; A61L27/00; A61L27/02; A61L27/025; A61L27/10; A61L27/12; A61L27/36; A61L27/3604; A61L27/3608; A61L27/3641; A61L27/3645; A61L27/365; A61L27/38; A61L27/3804; A61L27/3821; A61L27/3839; A61L27/3843; A61L27/3847; C03C3/00; C03C3/04; C03C3/076; C03C3/097; C03C4/00; C03C4/0007; C12N5/0068; C12N5/0654; A61F2310/00; A61F2310/00005; A61F2310/00179; A61F2310/00293; A61F2310/00329; A61L2430/00; A61L2430/02; C12N2533/00; C12N2533/10; C12N2533/12; Y; Y10; Y10S; Y10S623/00; Y10S623/92
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
60 words
A bioactive material for the in vitro inoculation of cells which express an osteoblastic phenotype comprising a substrate of bioactive glass or ceramic, said substrate having been treated prior to inoculation by immersion in a buffer solution containing a concentration of ions typical of interstitial fluid followed by immersion in a solution containing biological molecules normally occurring in bone tissue.
Claim 2
Dependent Claim
60 words
A bioactive material for the in vitro inoculation of cells which express an osteoblastic phenotype comprising a substrate of bioactive glass or ceramic, said substrate having been treated prior to inoculation by immersion in a buffer solution containing a concentration of ions typical of interstitial fluid followed by immersion in a solution containing biological molecules normally occurring in bone tissue.
Claim 3
Dependent Claim
29 words
The bioactive material of claim 1, wherein the glass comprises a non-crystalline glass consisting essentially of: SiO 2 ; CaO; Na 2 O; and P 2 O 5 .
Claim 4
Dependent Claim
35 words
The bioactive material of claim 2 having the following composition: 40-50% by weight SiO 2 ; 20-30% by weight CaO; 5-30% by weight Na 2 O; and 0-12% by weight P 2 O 5 .
Claim 5
Dependent Claim
34 words
The glass of claim 2 having the following composition: 45% by weight SiO 2 ; 24.5% by weight CaO; 24.5% by weight Na 2 O; and 6% by weight P 2 O 5 .
Claim 6
Dependent Claim
11 words
The bioactive material of claim 1, wherein the material is porous.
Claim 7
Dependent Claim
13 words
The bioactive material of claim 5, wherein the porosity is between about 10-80%.
Claim 8
Dependent Claim
13 words
The bioactive material of claim 5, wherein the porosity is between about 20-30%.
Claim 9
Dependent Claim
14 words
The material of claim 7, wherein the pore size is less than 800 μm.
Claim 10
Dependent Claim
14 words
The material of claim 8, wherein the pore size range is about 75-200 μm.
Claim 11
Dependent Claim
29 words
The bioactive material of claim 1 where said buffer solution is modified tris buffer and said solution containing biological molecules normally occurring in bone tissue is tissue culture medium.
Claim 12
Dependent Claim
29 words
The bioactive material of claim 1 wherein the cells which express an osteoblastic phenotype are selected from the group consisting of osteoblasts and cells which are precursors to osteoblasts.
Claim 13
Dependent Claim
74 words
An implantable bone tissue replacement comprising a substrate of bioactive glass or ceramic around which bone formation takes place and bone tissue formed on the substrate in vitro by inoculation with cells which express an osteoblastic phenotype, said substrate having been treated prior to inoculation by immersion in a buffer solution containing a concentration of ions typical of interstitial fluid followed by immersion in a solution containing biological molecules normally occurring in bone tissue.
Claim 14
Dependent Claim
74 words
An implantable bone tissue replacement comprising a substrate of bioactive glass or ceramic around which bone formation takes place and bone tissue formed on the substrate in vitro by inoculation with cells which express an osteoblastic phenotype, said substrate having been treated prior to inoculation by immersion in a buffer solution containing a concentration of ions typical of interstitial fluid followed by immersion in a solution containing biological molecules normally occurring in bone tissue.
Claim 15
Dependent Claim
15 words
The implantable bone tissue replacement of claim 12 wherein the substrate comprises a porous material.
Claim 16
Dependent Claim
18 words
The implantable bone tissue replacement of claim 12 wherein the substrate is in the form of a sheet.
Claim 17
Dependent Claim
17 words
The implantable bone tissue replacement of claim 12 wherein the substrate is in the form of granules.
Claim 18
Dependent Claim
23 words
The implantable bone tissue replacement of claim 42 further comprising a prosthesis wherein said substrate comprises at least a portion of said prosthesis.
Claim 19
Dependent Claim
31 words
The implantable bone tissue replacement of claim 12 where said buffer solution is modified tris buffer and said solution containing biological molecules normally occurring in bone tissue is tissue culture medium.
Claim 20
Dependent Claim
31 words
The implantable bone tissue replacement of claim 12 wherein the cells which express an osteoblastic phenotype are selected from the group consisting of osteoblasts and cells which are precursors to osteoblasts.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2000-06-30 | 2000-12-30 | 2001-07-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2004-06-30 | 2004-12-30 | 2005-07-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2008-06-30 | 2008-12-30 | 2009-07-01 | $7700.00 | Window Closed / Lapsed |