EP0426641A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Promoter for transgenic plants
Assignee: Monsanto Technology LLC
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Filing: 1990-10-29
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Granted: —
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Expires: 2010-10-29
Abstract
Abstract A full-length transcript promoter from figwort mosaic virus (FMV) is identified and its DNA sequence given. The promoter functions as a strong and uniform promoter for chimeric genes inserted into plant cells. This strong promoter function is exhibited by a histochemical assay in floral buds and by reproductive scores of transgenic plants including the promoter. The promoter preferably includes a 5′ leader sequence that may be from the FMV itself or from a heterologous source with respect to the promoter. The promoter is used in a plant cassette vector, a chimeric gene and in methods for transforming plant cells to obtain transgenic plants, plant cells or seeds incorporating the FMV promoter.
Inventors
- 👤 Stephen Gary Rogers
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 15:46
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216; C12N15/8222; C12N2730/00; C12N2730/00011; C12N2730/00022
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
8 words
A full-length transcript promoter from figwort mosaic virus.
Claim 2
Dependent Claim
8 words
A full-length transcript promoter from figwort mosaic virus.
Claim 3
Dependent Claim
22 words
The promoter of claim 1 wherein said promoter has the nucleotide sequence as shown in nucleotides 6368 to 6930 of Figure 1.
Claim 4
Dependent Claim
16 words
A promoter of claim 1 further comprising a 5′ non-translated leader sequence from figwort mosaic virus.
Claim 5
Dependent Claim
21 words
A promoter of claim 1 further comprising a 5′ non-translated leader sequence from a source heterologous with respect to the promoter.
Claim 6
Dependent Claim
17 words
The promoter of claim 3 wherein said promoter has the nucleotide sequence as shown in Figure 1.
Claim 7
Independent Claim
27 words
A method for transforming a plant cell to express a chimeric gene, the improvement comprising a chimeric gene containing a full-length transcript promoter from figwort mosaic virus.
Claim 8
Independent Claim
27 words
A method for transforming a plant cell to express a chimeric gene, the improvement comprising a chimeric gene containing a full-length transcript promoter from figwort mosaic virus.
Claim 9
Dependent Claim
14 words
A method of claim 6 wherein said promoter includes a 5′ non-translated leader sequence.
Claim 10
Dependent Claim
16 words
A method of claim 7 wherein said 5′ non-translated leader sequence is from figwort mosaic virus.
Claim 11
Dependent Claim
21 words
A method of claim 7 wherein said 5′ non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 12
Dependent Claim
58 words
A chimeric gene that functions in plant cells comprising: a full-length transcript promoter from figwort mosaic virus; a structural DNA sequence that is heterologous with respect to the promoter; and a 3′ non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of the RNA.
Claim 13
Dependent Claim
58 words
A chimeric gene that functions in plant cells comprising: a full-length transcript promoter from figwort mosaic virus; a structural DNA sequence that is heterologous with respect to the promoter; and a 3′ non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of the RNA.
Claim 14
Dependent Claim
16 words
The chimeric gene of claim 10 wherein said promoter further comprises a 5′ non-translated leader sequence.
Claim 15
Dependent Claim
17 words
The chimeric gene of claim 11 wherein said 5′ non-translated leader sequence is from figwort mosaic virus.
Claim 16
Dependent Claim
22 words
The chimeric gene of claim 11 wherein said 5′ non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 17
Dependent Claim
47 words
A chimeric gene of claim 10 wherein said structural DNA sequence comprises a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell.
Claim 18
Dependent Claim
59 words
A transformed plant cell that contains a chimeric gene comprising: a full-length transcript promoter from figwort mosaic virus; a structural DNA sequence that is heterologous with respect to said promoter; and a 3′ non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of the RNA.
Claim 19
Dependent Claim
59 words
A transformed plant cell that contains a chimeric gene comprising: a full-length transcript promoter from figwort mosaic virus; a structural DNA sequence that is heterologous with respect to said promoter; and a 3′ non-translated region which encodes a polyadenylation signal which functions in plant cells to cause the addition of polyadenylate nucleotides to the 3′ end of the RNA.
Claim 20
Dependent Claim
16 words
A plant cell of claim 15 wherein said promoter further comprises a 5′ non-translated leader sequence.
Claim 21
Dependent Claim
18 words
A transformed plant cell of claim 16 wherein said 5′ non-translated leader sequence is from figwort mosaic virus.
Claim 22
Dependent Claim
23 words
A transformed plant cell of claim 16 wherein said 5′ non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 23
Dependent Claim
49 words
A transformed plant cell of claim 15 wherein said structural DNA sequence further comprises a coding sequence which causes the production of RNA, encoding a chloroplast transit peptide/5-enolpyruvylshikimate-3-phosphate synthase fusion polypeptide, which chloroplast transit peptide permits the fusion polypeptide to be imported into a chloroplast of a plant cell.
Claim 24
Dependent Claim
18 words
A transformed plant cell of claim 19 wherein the coding sequence encodes a glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
Claim 25
Dependent Claim
18 words
A transformed plant cell of claim 19 wherein the chloroplast transit peptide is from a plant EPSPS gene.
Claim 26
Dependent Claim
16 words
A chimeric gene of claim 14 wherein the coding sequence encodes a glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
Claim 27
Dependent Claim
17 words
A chimeric gene of claim 14 wherein the chloroplast transit peptide is from a plant EPSPS gene.
Claim 28
Dependent Claim
53 words
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens which is capable of inserting a chimeric gene into susceptible plant cells, wherein said chimeric gene comprises a full-length transcript promoter from figwort mosaic virus and a structural DNA sequence that is heterologous with respect to the promoter.
Claim 29
Dependent Claim
53 words
A plant transformation vector which comprises a disarmed plant tumor inducing plasmid of Agrobacterium tumefaciens which is capable of inserting a chimeric gene into susceptible plant cells, wherein said chimeric gene comprises a full-length transcript promoter from figwort mosaic virus and a structural DNA sequence that is heterologous with respect to the promoter.
Claim 30
Dependent Claim
17 words
A plant transformation vector of claim 24 wherein said promoter further comprises a 5′ non-translated leader sequence.
Claim 31
Dependent Claim
18 words
A plant transformation vector of claim 25 wherein said 5′ non-translated leader sequence is from figwort mosaic virus.
Claim 32
Dependent Claim
23 words
A plant transformation vector of claim 25 wherein said 5′ non-translated leader sequence is from a source heterologous with respect to the promoter.
Claim 33
Dependent Claim
14 words
A transgenic plant which comprises plant cells of any of Claims 15 to 21.
Claim 34
Dependent Claim
8 words
A seed from a plant of Claim 28.
References Cited (Backward Citations)
US4940835A
Cited
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