Patent US5359142A successfully refreshed from Google Patents.
US5359142A
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Method for enhanced expression of a protein
Assignee: Monsanto Technology LLC
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Filing: 1994-03-09
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Granted: 1994-10-25
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Expires: 2014-03-09
Abstract
Abstract Novel transcription initiation regions that provide for enhanced transcription of a DNA sequence, particularly a plant sequence, are provided.
Inventors
- 👤 Joan C. McPherson
- 👤 Robert Kay
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8216
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
62 words
A differentiated plant comprising that comprise a DNA construct having as components (a) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (b) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (c) a nucleotide sequence of interest; and (d) a termination region; wherein said components are operably joined.
Claim 2
Dependent Claim
62 words
A differentiated plant comprising that comprise a DNA construct having as components (a) a tandemly duplicated CaMV 35S enhancer sequence comprising an AluI-EcoRV fragment of a CaMV 35S upstream region; and (b) a promoter comprising an RNA polymerase binding site and an mRNA initiation site; (c) a nucleotide sequence of interest; and (d) a termination region; wherein said components are operably joined.
Claim 3
Dependent Claim
14 words
The differentiated plant of claim 1 wherein said promoter is a CaMV 35S promoter.
Claim 4
Dependent Claim
16 words
The differentiated plant of claim 1 wherein said nucleotide sequence of interest encodes a selection marker.
Claim 5
Dependent Claim
14 words
The differentiated plant of claim 3 wherein said selection marker is neomycinphosphotransferase type II.
Claim 6
Dependent Claim
18 words
The differentiated plant of claim 1 wherein said nucleotide sequence of interest encodes a protein providing herbicide resistance.
Claim 7
Dependent Claim
16 words
The differentiated plant of claim 1 wherein said nucleotide sequence of interest encodes an antisense RNA.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-10-24 | 1998-04-25 | 1998-10-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-10-24 | 2002-04-25 | 2002-10-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-10-24 | 2006-04-25 | 2006-10-25 | $7700.00 | Window Closed / Lapsed |