US5310881A
Expired / Lapsed
US
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Glycosaminoglycan-modified protein
Assignee: Seikagaku Corp
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Filing: 1992-07-02
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Granted: 1994-05-10
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Expires: 2012-07-02
Abstract
Abstract A glycosaminoglycan-modified protein wherein an amino group of a protein is bound to an aldehyde group, which has been formed by reducing and partially oxidizing the reducing terminal sugar moiety of a glycosaminoglycan, which has a high stability in vivo and can retain physiological activity of the protein for a prolonged period of time.
Inventors
- 👤 Katsukiyo Sakurai
- 👤 Kyosuke Miyazaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:12
CPC Classifications
C; C07; C07K; C07K14/00; C07K1/00; C07K1/107; C07K1/1072; C07K1/1077; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/61; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C12; C12N; C12N9/00; C12N9/96; A61K38/00
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
47 words
A glycosaminoglycan-modified protein represented by the formula: ##STR13## wherein P represents a protein residue exclusive of n amino groups from the protein; n is an integer of from 1 to 100; and GAG represents a glycosaminoglycan residue exclusive of a reducing terminal sugar moiety from the glycosaminoglycan.
Claim 2
Dependent Claim
47 words
A glycosaminoglycan-modified protein represented by the formula: ##STR13## wherein P represents a protein residue exclusive of n amino groups from the protein; n is an integer of from 1 to 100; and GAG represents a glycosaminoglycan residue exclusive of a reducing terminal sugar moiety from the glycosaminoglycan.
Claim 3
Dependent Claim
25 words
The glycosaminoglycan-modified protein according to claim 1, wherein the glycosaminoglycan content ranges from 1 to 99.9% by weight based on the weight of the protein.
Claim 4
Dependent Claim
34 words
The glycosaminoglycan-modified protein according to claim 1, wherein the glycosaminoglycan is selected from the group consisting of colominic acid, hyaluronic acid, chondroitin, chondroitin sulfate, teichuronic acid, dermatan sulfate, heparin, heparan sulfate, keratosulfate and keratopolysulfate.
Claim 5
Dependent Claim
49 words
A process for the production of a glycosaminoglycan-modified protein of claim 1, which comprises activating a glycosaminoglycan by partially oxidizing the reducing terminal sugar moiety of said glycosaminoglycan to thereby cleave the terminal sugar moiety followed by forming a lactone therefrom and reacting the activated glycosaminoglycan with a protein.
Claim 6
Dependent Claim
17 words
A pharmaceutical composition containing a glycosaminoglycan-modified protein of claim 1, and a pharmaceutically acceptable carrier or diluent.
Claim 7
Dependent Claim
38 words
A glycosaminoglycan-modified protein wherein a reducing terminal sugar moiety of the glycosaminoglycan is cleaved and a functional group of the reducing terminal sugar moiety-cleaved glycosaminoglycan is bound to an amino group of a protein via a covalent bond.
Claim 8
Dependent Claim
38 words
A glycosaminoglycan-modified protein wherein a reducing terminal sugar moiety of the glycosaminoglycan is cleaved and a functional group of the reducing terminal sugar moiety-cleaved glycosaminoglycan is bound to an amino group of a protein via a covalent bond.
Claim 9
Dependent Claim
44 words
A glycosaminoglycan-modified protein wherein an amino group of a protein is bound to a carbonyl group of the glycosaminoglycan, which has been formed by oxidizing a reducing terminal sugar moiety of a glycosaminoglycan and treating the reducing terminal sugar moiety-oxidized glycosaminoglycan with an acid.
Claim 10
Dependent Claim
44 words
A glycosaminoglycan-modified protein wherein an amino group of a protein is bound to a carbonyl group of the glycosaminoglycan, which has been formed by oxidizing a reducing terminal sugar moiety of a glycosaminoglycan and treating the reducing terminal sugar moiety-oxidized glycosaminoglycan with an acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-05-09 | 1997-11-08 | 1998-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-05-09 | 2001-11-08 | 2002-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-05-09 | 2005-11-08 | 2006-05-10 | $7700.00 | Window Closed / Lapsed |