Patent US4625722A successfully refreshed from Google Patents.
US5284940A
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Preparation for nucleic acid samples
Assignee: Cerus Corp
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Filing: 1990-11-14
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Granted: 1994-02-08
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Expires: 2010-11-14
Abstract
Abstract Methods and compounds for the preparation of nucleic acid samples. Polymerase inhibitors are treated so as to allow for amplification of nucleic acid from biological sources, including whole blood.
Inventors
- 👤 Lily Lin
- 👤 Stephen T. Isaacs
- 👤 John E. Hearst
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:44
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/701; C12Q1/702; C12Q1/703; C07; C07K; C07K16/00; C07K16/44; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/1003; C12Q1/68; C12Q1/6806; C12Q1/6844; C12Q1/6848
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
34 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspecting of containing a polymerase inhibitor; and b) adding transferrin to said nucleic acid to neutralize said polymerase inhibitor.
Claim 2
Independent Claim
34 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspecting of containing a polymerase inhibitor; and b) adding transferrin to said nucleic acid to neutralize said polymerase inhibitor.
Claim 3
Dependent Claim
22 words
The method for preparing nucleic acid samples of claim 1, further comprising, after step (b), the step of amplifying said nucleic acid.
Claim 4
Dependent Claim
42 words
The method for preparing nucleic acid samples as in claim 1 wherein said transferrin is used in conjunction with one or more cofactors selected from the group consisting of bicarbonate ion, thiocyanate ion, cyanate ion, glycinate ion, thioglycolate ion, and salts thereof.
Claim 5
Independent Claim
65 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspecting of containing a polymerase inhibitor; and b) adding globin to said nucleic acid to neutralize said polymerase inhibitor in the presence of a cofactor selected from the group consisting of bicarbonate ion, azide ion, thiocyanate ion, cyanate ion, oxalate ion, malonate ion, glycinate ion, thioglycolate ion and salts thereof.
Claim 6
Independent Claim
65 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspecting of containing a polymerase inhibitor; and b) adding globin to said nucleic acid to neutralize said polymerase inhibitor in the presence of a cofactor selected from the group consisting of bicarbonate ion, azide ion, thiocyanate ion, cyanate ion, oxalate ion, malonate ion, glycinate ion, thioglycolate ion and salts thereof.
Claim 7
Dependent Claim
22 words
The method for preparing nucleic acid samples of claim 4, further comprising, after step (b), the step of amplifying said nucleic acid.
Claim 8
Independent Claim
66 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspected of containing a polymerase inhibitor; and b) adding serum albumin to said nucleic acid to neutralize said polymerase inhibitor in the presence of a cofactor selected from the group consisting of bicarbonate ion, azide ion, thiocyanate ion, cyanate ion, oxalate ion, malonate ion, glycinate ion, thioglycolate ion, and salts thereof.
Claim 9
Independent Claim
66 words
A method for preparing nucleic acid samples comprising the steps of: a) providing nucleic acid suspected of containing a polymerase inhibitor; and b) adding serum albumin to said nucleic acid to neutralize said polymerase inhibitor in the presence of a cofactor selected from the group consisting of bicarbonate ion, azide ion, thiocyanate ion, cyanate ion, oxalate ion, malonate ion, glycinate ion, thioglycolate ion, and salts thereof.
Claim 10
Dependent Claim
22 words
The method for preparing nucleic acid samples of claim 6, further comprising, after step (b), the step of amplifying said nucleic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1997-02-07 | 1997-08-09 | 1998-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2001-02-07 | 2001-08-09 | 2002-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2005-02-07 | 2005-08-09 | 2006-02-08 | $7700.00 | Window Closed / Lapsed |