US3874852A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent and method for determining leukocytes and hemoglobin in the blood
Assignee: Beckman Coulter Diagnostics Inc
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Filing: 1974-07-05
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Granted: 1975-04-01
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Expires: 1994-07-05
Abstract
Abstract Leukocytes and hemoglobin in the blood are determined in vitro with a reagent which comprises a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion in amounts sufficient to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for the determinations.
Inventors
- 👤 Thomas E Hamill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5094; G01N33/72; G01N33/721; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
71 words
A REAGENT FOR USE IN THE DETERMINATION OF LEUKOCYTES AND HEMOGLOBIN IN THE BLOOD, EHICH COMRPISES A FERRICYANIDE ION-FREE AQUEOUS SOLUTION CONTAINING A QUATERNARY AMMONIUM ION AND CYANIDE ION, SAID QUATERNARY AMMONIUM ION HAVING ATTACHED TO NITROGEN THREE SHORT CHAIN ALKYL GROUP AND ONE LONG CHAIN ALKYL GROUP, AND SAID IONS BEING PRESENT IN AMOUNTS SUFFSUFFICIENT TO STROMATOLYSE ERYTHROCYTE AND PLATELET CELLS AND CONVERT HEMOGOBLIN TO A CHROMOGEN FOR SAID DETERMINATIONS.
Claim 2
Dependent Claim
71 words
A REAGENT FOR USE IN THE DETERMINATION OF LEUKOCYTES AND HEMOGLOBIN IN THE BLOOD, EHICH COMRPISES A FERRICYANIDE ION-FREE AQUEOUS SOLUTION CONTAINING A QUATERNARY AMMONIUM ION AND CYANIDE ION, SAID QUATERNARY AMMONIUM ION HAVING ATTACHED TO NITROGEN THREE SHORT CHAIN ALKYL GROUP AND ONE LONG CHAIN ALKYL GROUP, AND SAID IONS BEING PRESENT IN AMOUNTS SUFFSUFFICIENT TO STROMATOLYSE ERYTHROCYTE AND PLATELET CELLS AND CONVERT HEMOGOBLIN TO A CHROMOGEN FOR SAID DETERMINATIONS.
Claim 3
Dependent Claim
57 words
A reagent according to claim 1 wherein said quaternary ammonium ion is provided by a quaternary ammonium salt incorporated in said solution in a concentration in the range of about 0.5 to 10 percent by weight of said solution, and said cyanide ion is present in a concentration in the range of about 0.0006 to 0.005 molar.
Claim 4
Dependent Claim
34 words
A reagent according to claim 2 wherein said salt is a trimethyl tetradecyl ammonium halide, sulfate, phosphate, or nitrate, and said cyanide ion is provided by an alkali metal cyanide incorporated in said solution.
Claim 5
Dependent Claim
33 words
A reagent according to claim 1 wherein said short chain alkyl groups each have from 1 to 3 carbon atoms and said long chain alkyl group has from 10 to 14 carbon atoms.
Claim 6
Dependent Claim
66 words
A reagent according to claim 4 wherein said quaternary ammonium ion is provided by a quaternary ammonium salt incorporated in said solution in a concentration in the range of about 0.5 to 10 percent by weight of said solution, and said cyanide ion is provided by an alkali metal cyanide incorporated in said solution in a concentration in the range of about 0.0006 to 0.005 molar.
Claim 7
Dependent Claim
16 words
A reagent according to claim 5 wherein said salt is a halide, sulfate, phosphate, or nitrate.
Claim 8
Dependent Claim
16 words
A reagent according to claim 1 comprising a composition having the following ingredients and relative proportions:
Claim 9
Dependent Claim
76 words
In a method of determining leukocytes and hemoglobin in the blood wherein a reagent is reacted with a blood sample to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for said determinations, the improvement which comprises employing as said reagent a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion, said quaternary ammonium ion having attached to nitrogen three short chain alkyl groups and one long chain alkyl group.
Claim 10
Dependent Claim
76 words
In a method of determining leukocytes and hemoglobin in the blood wherein a reagent is reacted with a blood sample to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for said determinations, the improvement which comprises employing as said reagent a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion, said quaternary ammonium ion having attached to nitrogen three short chain alkyl groups and one long chain alkyl group.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-31 | 1978-09-30 | 1979-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-31 | 1982-09-30 | 1983-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-31 | 1986-09-30 | 1987-04-01 | $7700.00 | Window Closed / Lapsed |