Reservoir chemical sensors

Claim 2

A reservoir chemical sensor comprising: a modular reservoir cell body made of acetal theremoplastic polymer; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent. a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adaptor means at each end of he cell for mounting and aligning the light source and detector to the cell body.

Claim 3

The sensor of claim 1 wherein the sensing reagent is a solution filling the reservoir cell body.

Claim 4

The sensor of claim 2 further comprising a transparent, protective window between the cell body and at least one of the source and detector.

Claim 5

The sensor of claim wherein the window has a surface energy which matches the surface tension of the reagent solution to prevent bubble formation.

Claim 6

The sensor of claim 1 wherein the light source and detector are aligned along a cell axis.

Claim 7

The sensor of claim 1 wherein the detector is aligned in a direction substantially at a right angle to the light source.

Claim 8

The sensor of claim 1 further comprising a color filter between the cell body and at least one of the source and detector.

Claim 9

The sensor of claim 1 further comprising a focusing or collimating lens between the cell body and at least one of the source and detector.

Claim 10

The sensor of claim 1 wherein the species communication means comprises an open port.

Claim 11

The sensor of claim 1 further comprising a pair of space parallel membranes across the cell body and enclosing the sensing reagent.

Claim 12

The sensor of claim 1 wherein the sensing reagent is immobilized in the cell body.

Claim 13

The sensor of claim 1 wherein the modular cell body has a volume of about 10 to about 200 microliters.

Claim 14

The sensor of claim 1 wherein the light source is selected form a diode, laser and lamp.

Claim 15

The sensor of claim 1 wherein the light source is a light emitting diode.

Claim 16

The sensor of claim 14 wherein the detector is a photodiode.

Claim 17

The sensor of claim 1 wherein the light source is a lamp surrounded by a fiber optic bundle.

Claim 18

The sensor of claim 1 wherein the sensing reagent is selected from the group consisting of: (a) phenol red, cresol red, methyl violet, congo red, phenolphthalein and bromcresol purple; pH sensitive absorption dyes and pH sensitive fluorescent dyes; (b) the reagents of (a) in combination with eosin; (c) fluorescein, acridine, umbelliferone and beta-naphthol; (d) thorium-morin chelate, a luminol-(NH 4 ) 2 MoO 4 -NH 4 NO 3 mixture, an ammonium molybdate/stannous chloride mixture and an N-ethyl-8-hydroxtetrahydroquinoline/ferric chloride mixture; (e) a solvent for benzene with a lower refractive index than benzene; (f) p-benzoquinone; (g) cupric neocuprine; (h) 2,2'-dipyridylketone hyrazone solution and lumpocupferron solution; (i) a solvent for TCE with a lower refractive index than TCE; (j) 2,2'-dipyridylketone hydrazone solution and indole-3-propionic acid; (k) ferrozine; (l) bis(2,4,6 trichlorophenyl) oxalate-H 2 O 2 -per-5ylene, acidified diphenylcarbazide, and a lphine/KOH/H 2 O 2 mixture; (m) 2,3-diaminmonaphthalene, 3,3'-diaminobenzidine, and dithizone; (n) 2,3 napthotriazole and a 1,10 phenanthroline/eosin mixture; (o) butylrhodamine B/chloride mixture and 5-hydroxy-2-hydroxmethyl-1,4-pyrone; (p) N-(4-methylumbelliferone-8-methyl)-1,10-diazo-18-crown-6; q) p-nitrophenyl hydrazine; (r) benzidine and tetramethyldiaminotriphenylmethane; (s) acidified vanillin and an alcohol dehydrogenase/zinc hydroxide mixture; (t) dihydroacridane and indigo blue; (u) Malachite green; (v) red thorin-barium complex; (w) a mixture of MM and BB monoclonal antibodies and MB polyclonal antibody tagged with a fluorescent compound; (x) antibodies specific to cocaine, caffeine, chlordane, methadone, morphine, hepatitis and influenza; (y) an enzyme selected form 3α-hydroxysteroid dehydrogenase, pyruvate dehydrogenase, aldehyde dehydrogenase and cholesterol dehydrogenase.

Claim 19

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body and aligned with the light source along a cell axis top detect effects produced by the interaction of the species of interest with the sensing reagent; at least one additional detector mounted to the cell body and aligned in a direction substantially at a right angle to the cell axis; an adapter means for mounting and aligning each light source and detector to the cell body.

Claim 20

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body and aligned with the light source along a cell axis top detect effects produced by the interaction of the species of interest with the sensing reagent; at least one additional detector mounted to the cell body and aligned in a direction substantially at a right angle to the cell axis; an adapter means for mounting and aligning each light source and detector to the cell body.

Claim 21

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent wherein the species communication means comprises a flow-through port on a lateral surface of the cell body and a species-permeable, sensing-reagent-impermeable membrane across the flow-through port and a membrane retaining means for holding the membrane in place across the flow-through port and maintaining a liquid tight seal; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body; wherein the membrane and sensing reagent are selected from the group consisting of the following combinations: (a) the membrane is a hydrogen ion/hydronium ion permeable membrane and the sensing reagent is a pH sensitive absorption dye in combination with eosin; (b) the membrane is an arsenic ion permeable membrane and the sensing reagent is selected from thorium-morin chelate, a luminol-(NH 4 ) 2 MoO 4 -NH 4 NO 3 mixture, an ammonium molybdate/stannous chloride mixture and an N-ethyl-8-hydroxtetrahydroquinoline/ferric chloride mixture; (c) the membrane is selected form high density polyethylene, high density polypropylene and fluorinated forms thereof, and the sensing reagent is a solvent for benzene with refractive index lower than benzene; (d) the membrane is a cyanide permeabel membrane and the sensing reagent is p-benzene; (e) the membrane is a hydrazine permeable membrane and the sensing reagent is cupric neocuprine solution; (f) the membrane is a cupric ion permeable membrane and the sensing reagent is selected form 2,2'-dipyridylketone hydrazone solution and lumpocupferron solution; (g) the membrane is a trichloroethylene permeable silicone polymer and the sensing reagent is a solvent for trichloroethylene with refractive index lower than trichloroethylene; (h) the membrane is a mercuric ion permeable membrane and the sensing reagent is selected form 2,2'-dipyridylketone hydrazone solution and indole-3- propionic acid; (i) the membrane is an iron(2+) permeable membrane and the sensing reagent is ferozine solution; (j) the membrane is a chromium permeate membrane and the sensing reagent is selected from bis)2,4,6 trichlorophenyl) oxalate-H 2 O 2 -per-5ylene, acidified diphenycarbazide, and a lophine/KOH/H 2 O 2 mixture; (k) the membrane is a selenium permeable membrane and the sensing reagent is selected form 2,3 napthothriazole and a 1,10 phenanthroline/eosin mixture; (m) the membrane is a gold permeable membrane and the sensing reagent is selected form butylrodamein B/chloride mixture and 5-hydrosy-2-hydroxmethyl-1,4-pyrone; (n) the membrane is a calcium permeable membrane and the sensing reagent is N-(4methylumbelliferone-8-methyl)-1,10-diazo-18-crown-6;

Claim 22

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent wherein the species communication means comprises a flow-through port on a lateral surface of the cell body and a species-permeable, sensing-reagent-impermeable membrane across the flow-through port and a membrane retaining means for holding the membrane in place across the flow-through port and maintaining a liquid tight seal; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body; wherein the membrane and sensing reagent are selected from the group consisting of the following combinations: (a) the membrane is a hydrogen ion/hydronium ion permeable membrane and the sensing reagent is a pH sensitive absorption dye in combination with eosin; (b) the membrane is an arsenic ion permeable membrane and the sensing reagent is selected from thorium-morin chelate, a luminol-(NH 4 ) 2 MoO 4 -NH 4 NO 3 mixture, an ammonium molybdate/stannous chloride mixture and an N-ethyl-8-hydroxtetrahydroquinoline/ferric chloride mixture; (c) the membrane is selected form high density polyethylene, high density polypropylene and fluorinated forms thereof, and the sensing reagent is a solvent for benzene with refractive index lower than benzene; (d) the membrane is a cyanide permeabel membrane and the sensing reagent is p-benzene; (e) the membrane is a hydrazine permeable membrane and the sensing reagent is cupric neocuprine solution; (f) the membrane is a cupric ion permeable membrane and the sensing reagent is selected form 2,2'-dipyridylketone hydrazone solution and lumpocupferron solution; (g) the membrane is a trichloroethylene permeable silicone polymer and the sensing reagent is a solvent for trichloroethylene with refractive index lower than trichloroethylene; (h) the membrane is a mercuric ion permeable membrane and the sensing reagent is selected form 2,2'-dipyridylketone hydrazone solution and indole-3- propionic acid; (i) the membrane is an iron(2+) permeable membrane and the sensing reagent is ferozine solution; (j) the membrane is a chromium permeate membrane and the sensing reagent is selected from bis)2,4,6 trichlorophenyl) oxalate-H 2 O 2 -per-5ylene, acidified diphenycarbazide, and a lophine/KOH/H 2 O 2 mixture; (k) the membrane is a selenium permeable membrane and the sensing reagent is selected form 2,3 napthothriazole and a 1,10 phenanthroline/eosin mixture; (m) the membrane is a gold permeable membrane and the sensing reagent is selected form butylrodamein B/chloride mixture and 5-hydrosy-2-hydroxmethyl-1,4-pyrone; (n) the membrane is a calcium permeable membrane and the sensing reagent is N-(4methylumbelliferone-8-methyl)-1,10-diazo-18-crown-6;

Claim 23

The sensor of claim 19 further comprising a sample preparation chamber adjacent to the modular cell body and having the semi-permeable membrane as an interface between the sample preparation chamber and the cell body.

Claim 24

The senor of claim 20 wherein the sample preparation chamber has an inlet port and an outlet port for passing sample through the chamber and an irradiation port for irradiating the sample in the chamber.

Claim 25

The senor of claim 20 further comprising a chemical bed in the chamber for producing chemical reactions of the sample in the chamber.

Claim 26

The sensor of claim 19 further comprising a lateral sensing reagent inlet port and a lateral sensing reagent outlet port formed on a lateral surface in the cell body.

Claim 27

The sensor of claim 19 for arsenic wherein the membrane is an arsenic ion permeable membrane and the sensing solution is selected from thorium-morin chelate, a luminol-(NH 4 ) 2 MoO 4 -NH 4 NO 3 mixture, an ammonium molybdate/stannous chloride mixture and an N-ethyl-8hydroxtetrahydroquinoline/ferric chloride mixture.

Claim 28

The sensor of claim 19 for benzene wherein the membrane is selected form high density polyethylene, high density polyproplene and fluoridated forms thereof, and the sensing reagent is a solvent for benzene with refractive index lower than benzene.

Claim 29

The sensor of claim 19 for cyanide wherein the membrane is a cyanide permeate membrane and the sensing reagent is p-benzoquinone.

Claim 30

The sensor of claim 19 for hydrazine wherein the membrane is a hydrazine permeable membrane and the sensing reagent is cupric neocuprine solution.

Claim 31

The sensor of claim 19 for cupric ion wherein the membrane is a cupric ion permeable membrane and the sensing reagent is selected for 2,2'- dipyridylketone hydrazone solution and lumpocupferron solution.

Claim 32

The sensor of claim 19 for trichloroethylene wherein the membrane is a trichloroethlene permeable silicone polymer and the sensing reagent is a solvent for trichloroethylene with refractive index lower than trichloroethylene.

Claim 33

The sensor of claim 29 wherein the solvent is ethanol or acetone.

Claim 34

The sensor of claim 19 for mercuric ion wherein the membrane is a mercuric ion permeate membrane and the sensing solution is selected form 2,2'-dipyridylketone hydrazone solution and indole-3-proionic acid.

Claim 35

The sensor of claim 19 for iron (2+) wherein the membrane is an iron (2+) permeable membrane and the sensing reagent is ferrozine solution.

Claim 36

The sensor of claim 19 for chromium wherein the membrane is a chromium permeable membrane and the sensing reagent is selected form bis(2,4,6 trichlorophenyl)oxalate-H 2 O 2 -per-5ylene, acidified diphenycarbazide, and a lophine/KOH/H 2 O 2 mixture.

Claim 37

The sensor of claim 19 for selenium wherein the membrane is a selenium permeate membrane and the sensing reagent is selected from 2,3-diaminonaphthalene, 3,3'-diaminobezidine, and dithizone.

Claim 38

The sensor of claim 19 for silver wherein the membrane is a silver permeable membrane and the sensing reagent is selected from 2,3 napthotriazole and a 1,10 phenanthroline/eosin mixture.

Claim 39

The sensor of claim 19 for gold wherein the membrane is a gold permeable membrane and the sensing reagent is selected from butylrhodamine B/chloride and 5-hydroxy-2-hydroxmehtyl-1,4-pyrone.

Claim 40

The sensor of claim 19 for calcium wherein the membrane is a calcium permeable membrane and the sensing reagent is N-(4-methylumbelliferone-8-methyl) 1,10-diazo-18-crown-6.

Claim 41

The sensor of claim 19 for aldehydes wherein the membrane is an aldehyde permeable membrane and the sensing reagent is p-nitrophenyl hydrazine.

Claim 42

The sensor of claim 19 for blood wherein the membrane is a blood permeable membrane and the sensing reagent is selected from benzidine and tetramethyldiaminotriphenylmethane.

Claim 43

The sensor of claim 19 for alcohol wherein the membrane is an alcohol permeable membrane and the sensing reagent is selected from acidified vanillin and an alcohol dehydrogenase/zinc hydroxide mixture.

Claim 44

The sensor of claim 19 for ozone wherein the membrane is an ozone permeable membrane and the sensing reagent is selected from dihydroacridane and indigo blue.

Claim 45

The sensor of claim 19 for sulfite wherein the membrane is a sulfit porous membrane and the sensing reagent is malachite green.

Claim 46

The sensor of claim 19 for sulfate wherein the membrane is a sulfate porous membrane and the sensing reagent is ed thorin-barium complex.

Claim 47

The sensor of claim 19 for creatine phosphokinase wherein the membrane is a creatine phosphokinase permeable membrane and the sensing reagent is a mixture of MM, BB monclonal antibodies and MB polyclonal antibody.

Claim 48

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent and comprising a lateral sample inlet port and an opposed lateral outlet port on opposed lateral surfaces of the cell body for passing sample through the cell body; a lateral sensing reagent inlet port on a lateral surface of the cell body for replenishing reagent in the cell body; valves placed in the inlet and outlet ports to regulate or control flow; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body.

Claim 49

A reservoir chemical sensor comprising: a modular reservoir cell body; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent and comprising a lateral sample inlet port and an opposed lateral outlet port on opposed lateral surfaces of the cell body for passing sample through the cell body; a lateral sensing reagent inlet port on a lateral surface of the cell body for replenishing reagent in the cell body; valves placed in the inlet and outlet ports to regulate or control flow; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body.

Claim 50

A reservoir chemical sensor comprising: a modular reservoir cell body comprising adjacent cell body sections and a semi-permeable membrane mounted between adjacent cell body sections to form adjacent cell body chambers; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body; wherein the sensing reagent further comprises a first reagent in one cell body chamber and a second reagent in a second cell body chamber, wherein the first reagent is reactive with the species of interest to form a reaction product, and the second reagent is reactive to the reaction product, and the membrane is permeable to the reaction product and impermeable to the first and second reagents.

Claim 51

A reservoir chemical sensor comprising: a modular reservoir cell body comprising adjacent cell body sections and a semi-permeable membrane mounted between adjacent cell body sections to form adjacent cell body chambers; a sensing reagent in the cell body; species communication means formed in the cell body for passing a species of interest into the cell body to interact with the sensing reagent; a light source positioned at one end of the cell body to illuminate the interior of the cell body; a detector positioned at an opposed end of the cell body to detect effects produced by the interaction of the species of interest with the sensing reagent; an adapter means at each end of the cell for mounting and aligning the light source and detector to the cell body; wherein the sensing reagent further comprises a first reagent in one cell body chamber and a second reagent in a second cell body chamber, wherein the first reagent is reactive with the species of interest to form a reaction product, and the second reagent is reactive to the reaction product, and the membrane is permeable to the reaction product and impermeable to the first and second reagents.

Claim 52

The sensor of claim 46 wherein the light source and detector are positioned in the second chamber.

Claim 53

The sensor of claim 46 wherein the species communication means are formed in the first chamber.

Claim 54

The sensor of claim 46 for arsenic wherein the first reagent is ammonium molybdate and the second reagent is stannous chloride.

Claim 55

The sensor of claim 46 for arsenic wherein the first reagent is N-ethyl-8-hydroxtetrahydroquinoline and the second reagent is ferric chloride.