Patent US4658815A successfully refreshed from Google Patents.
US5043288A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immobilize molecular binding partners to contact activating supports
Assignee: Individual
•
Filing: 1988-06-20
•
Granted: 1991-08-27
•
Expires: 2008-06-20
Abstract
Abstract Molecular binding partners such as antibodies and haptens are immobilized to support materials that have the property of contact activation of blood protein coagulation. A binding partner is attached to a surface-active protein carrier and the resulting conjugate non-covalently adsorbs onto the surface of the support. The method provides for diagnostic assays of greater sensitivity and convenience.
Inventors
- 👤 Marvin A. Motsenbocker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N33/53; G01N33/543; G01N33/54306; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/948; G01N2333/95; G01N2333/964; G01N2333/96425; G01N2333/96427; G01N2333/9643; G01N2333/96433; G01N2333/96441; G01N2333/96458; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/821; Y10S530/00; Y10S530/808; Y10S530/81
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
109 words
A method of preparing an immobilized binding member-support composition by absorbing a binding member carrier conjugate to a support material, said method comprising the steps of; a. forming a binding member carrier conjugate from a surface active carrier protein selected from the group consisting of factor XII, high molecular weight Kininogen (HMWK), fibrinogen and fibronectin and a binding member selected from the group consisting of antibody, antigen, hapten, avidin and biotin; and, b. contacting a complement activating support material having a high molecular mass and containing multiple negative charges with a solution of dissolved binding member carrier conjugate in an amount sufficient to coat said complement activating support material.
Claim 2
Independent Claim
109 words
A method of preparing an immobilized binding member-support composition by absorbing a binding member carrier conjugate to a support material, said method comprising the steps of; a. forming a binding member carrier conjugate from a surface active carrier protein selected from the group consisting of factor XII, high molecular weight Kininogen (HMWK), fibrinogen and fibronectin and a binding member selected from the group consisting of antibody, antigen, hapten, avidin and biotin; and, b. contacting a complement activating support material having a high molecular mass and containing multiple negative charges with a solution of dissolved binding member carrier conjugate in an amount sufficient to coat said complement activating support material.
Claim 3
Dependent Claim
19 words
The method of claim 1, wherein said carrier protein is prepared by expression of recombinant DNA from cell culture.
Claim 4
Dependent Claim
26 words
The method of claim 1, wherein said carrier protein and said binding member are contiguous and are prepared by expression of recombinant DNA from cell culture.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein said complement activating support material is glass.
Claim 6
Dependent Claim
16 words
The method of claim 4 wherein said complement activating support material is in a fibrous form.
Claim 7
Dependent Claim
29 words
The method of claim 1 wherein after contact of said complement activating support material with said binding member carrier conjugate, said complement activating support material is washed and dried.
Claim 8
Independent Claim
166 words
A method of measuring an analyte concentration by a sandwich assay method, the method comprising the steps of: a. simultaneously incubating a sample containing analyte with a binding member carrier conjugate comprised of binding member specific for the analyte conjugated to carrier protein selected from the group consisting of factor XII, HMWK, fibrinogen and fibronectin, and a second conjugate comprised of a second binding member specific for the analyte conjugated to a signal producing species, resulting in a complex of binding member carrier conjugate-analyte-binding member signal producing species conjugate; b. contacting a complement activating support material having a high molecular mass and multiple negative charges with the complex of step a resulting in the absorption of the complex to the support; c. separating the complement activating support material having the complex of step a absorbed thereto from unabsorbed conjugates; and, d. detecting a signal from the signal producing species in proportion to the amount of binding member carrier conjugate-analyte-binding member signal producing species conjugate complexes formed.
Claim 9
Independent Claim
166 words
A method of measuring an analyte concentration by a sandwich assay method, the method comprising the steps of: a. simultaneously incubating a sample containing analyte with a binding member carrier conjugate comprised of binding member specific for the analyte conjugated to carrier protein selected from the group consisting of factor XII, HMWK, fibrinogen and fibronectin, and a second conjugate comprised of a second binding member specific for the analyte conjugated to a signal producing species, resulting in a complex of binding member carrier conjugate-analyte-binding member signal producing species conjugate; b. contacting a complement activating support material having a high molecular mass and multiple negative charges with the complex of step a resulting in the absorption of the complex to the support; c. separating the complement activating support material having the complex of step a absorbed thereto from unabsorbed conjugates; and, d. detecting a signal from the signal producing species in proportion to the amount of binding member carrier conjugate-analyte-binding member signal producing species conjugate complexes formed.
Claim 10
Dependent Claim
19 words
The method of claim 7, wherein said carrier protein is prepared by expression of recombinant DNA from cell culture.
Claim 11
Dependent Claim
25 words
The method of claim 7, wherein said carrier protein and said binding member are contiguous are prepared by expression of recombinant DNA from cell culture.
Claim 12
Dependent Claim
13 words
The method of claim 7 wherein said complement activating support material is glass.
Claim 13
Dependent Claim
16 words
The method of claim 10 wherein said complement activating support material is in a fibrous form.
Claim 14
Dependent Claim
103 words
In an ELISA assay method, including incubation of analyte with binding member immobilized to complement activating support material and with a second binding member conjugated to a signal producing species, and detection of a signal that is proportional to the amount of analyte, wherein the improvement comprises: inhibiting non-specific binding of binding member signal producing species conjugate to complement activating support material by incubating surface active protein selected from the group consisting of factor XII, HMWK, fibrinogen and fibronectin with complement activating support material in solution in an excess amount needed to bind all the available surface of the complement activating support material.
Claim 15
Dependent Claim
103 words
In an ELISA assay method, including incubation of analyte with binding member immobilized to complement activating support material and with a second binding member conjugated to a signal producing species, and detection of a signal that is proportional to the amount of analyte, wherein the improvement comprises: inhibiting non-specific binding of binding member signal producing species conjugate to complement activating support material by incubating surface active protein selected from the group consisting of factor XII, HMWK, fibrinogen and fibronectin with complement activating support material in solution in an excess amount needed to bind all the available surface of the complement activating support material.
Claim 16
Dependent Claim
20 words
The method of claim 12 wherein said surface active protein is prepared by expression of recombinant DNA from cell culture.
Claim 17
Dependent Claim
22 words
The method of claim 12 wherein said complement activating support material is of high molecular weight and contains multiple negative surface charges.
Claim 18
Dependent Claim
16 words
The method of claim 12 wherein said complement activating support material is in a fibrous form.
References Cited (Backward Citations)
US3556945A
Cited
US3519538A
Cited
US3652761A
Cited
US3983000A
Cited
US4363634A
Cited
US4657873A
Cited
US4731337A
Cited
US4777145A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-08-26 | 1995-02-25 | 1995-08-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-08-26 | 1999-02-25 | 1999-08-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-08-26 | 2003-02-25 | 2003-08-27 | $7700.00 | Window Closed / Lapsed |