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US3983000A
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Bonding proteins to inorganic supports
Assignee: Corning Glass Works
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Filing: 1976-04-01
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Granted: 1976-09-28
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Expires: 1996-04-01
Abstract
Abstract Proteins such as enzymes or antibodies can be immobilized in a biologically active state on various inorganic supports via an intermediate residue of o-dianisidine.
Inventors
- 👤 Ralph A. Messing
- 👤 Gerald Odstrchel
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 04:15
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/552; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/913; Y10S435/917; Y10S530/00; Y10S530/81; Y10S530/811
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
71 words
A method of bonding a biologically active protein to an inorganic support material to prepare an immobilized protein-support composite, the method comprising the steps of a. reacting a high surface area, water insoluble inorganic material having available surface oxide or hydroxyl groups with a solution of o-dianisidine to form residues of o-dianisidine on the surface; and b. reacting the reaction product of step (a) with an aqueous solution of the protein.
Claim 2
Independent Claim
71 words
A method of bonding a biologically active protein to an inorganic support material to prepare an immobilized protein-support composite, the method comprising the steps of a. reacting a high surface area, water insoluble inorganic material having available surface oxide or hydroxyl groups with a solution of o-dianisidine to form residues of o-dianisidine on the surface; and b. reacting the reaction product of step (a) with an aqueous solution of the protein.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the protein of step (b) is selected from enzymes and antibodies.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein the protein is an enzyme selected from lactase, Bacillus subtilis protease, and catalase.
Claim 5
Dependent Claim
13 words
The method of claim 2 wherein the protein is an antibody to thyroxine.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein the inorganic material is siliceous.
Claim 7
Dependent Claim
34 words
The method of claim 5 wherein the siliceous material is in particulate form having a surface area of at least about 0.2 m 2 /g and selected from silica, porous glass, and fritted glass.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein the o-dianisidine is in an aqueous solution.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein the o-dianisidine is in an organic solution.
Claim 10
Dependent Claim
32 words
The method of claim 1 wherein after the reaction of step (a) but prior to the reaction of step (b) the reaction product of step (a) is reacted with a glutaraldehyde solution.
Claim 11
Dependent Claim
28 words
The method of claim 1 wherein after the reaction of step (a) but prior to the reaction of step (b) the reaction product of step (a) is diazotized.
Claim 12
Dependent Claim
32 words
An immobilized protein composite comprising a biologically active protein bonded via an o-dianisidine residue to a high surface area, essentially water insoluble inorganic material in accordance with the method of claim 1.
Claim 13
Dependent Claim
14 words
The composite of claim 11 wherein the protein is selected from enzymes and antibodies.
References Cited (Backward Citations)
US3930951A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-09-28 | 1980-03-29 | 1980-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-09-28 | 1984-03-29 | 1984-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-09-28 | 1988-03-29 | 1988-09-28 | $7700.00 | Window Closed / Lapsed |