Patent US5034323A successfully refreshed from Google Patents.
US5034323A
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Genetic engineering of novel plant phenotypes
Assignee: BIONOVA HOLDING Corp (A DELAWARE CORPORATION)
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Filing: 1989-03-30
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Granted: 1991-07-23
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Expires: 2009-03-30
Abstract
Abstract Methods are provided for producing plants exhibiting one or more desired phenotypic traits. In particular, transgenotes are selected that comprise a DNA segment operably linked to a promoter, wherein transcription products of the segment are substantially homologous to corresponding transcripts of endogenous flavonoid biosynthetic pathway genes.
Inventors
- 👤 Richard A. Jorgensen
- 👤 Carolyn A. Napoli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:16
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8201; C12N15/8213; C12N15/8216; C12N15/8218; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/825; C12N9/10; C12N9/1025; C12N9/1029; C12N9/1037; C12N9/88; C12Y; C12Y101/00; C12Y101/01; C12Y101/01219
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
85 words
A method for modifying petunia flower color pattern comprising altering flower pigment production by transforming a petunia cell with a disabled Ti plasmid of Agrobacterium tumefaciens, wherein said disabled Ti plasmid comprises a substantially full length coding region of a chalcone synthase (CHS) gene segment which is operably linked to a promoter such that sense transcripts are produced, and wherein the segment is substantially homologous to an endogenous CHS gene and when transcribed in the transgenote is capable of effecting the flower color pattern modification.
Claim 2
Independent Claim
85 words
A method for modifying petunia flower color pattern comprising altering flower pigment production by transforming a petunia cell with a disabled Ti plasmid of Agrobacterium tumefaciens, wherein said disabled Ti plasmid comprises a substantially full length coding region of a chalcone synthase (CHS) gene segment which is operably linked to a promoter such that sense transcripts are produced, and wherein the segment is substantially homologous to an endogenous CHS gene and when transcribed in the transgenote is capable of effecting the flower color pattern modification.
Claim 3
Independent Claim
105 words
A method for modifying color pattern in a flower of a petunia plant having an endogenous chalcone synthase (CHS) gene, the method comprising, growing whole plant transgenotes from a cell, said transgenotes transformed with a recombinant nucleic acid sequence comprising a substantially full length coding region of a CHS gene segment operably linked to a promoter, wherein the segment is linked to the promoter such that sense transcripts are produced, the segment being substantially homologous to the endogenous CHS gene and when transcribed in the transgenote being capable of effecting the flower color pattern modification; and selecting a plant exhibiting the modified flower color pattern.
Claim 4
Independent Claim
105 words
A method for modifying color pattern in a flower of a petunia plant having an endogenous chalcone synthase (CHS) gene, the method comprising, growing whole plant transgenotes from a cell, said transgenotes transformed with a recombinant nucleic acid sequence comprising a substantially full length coding region of a CHS gene segment operably linked to a promoter, wherein the segment is linked to the promoter such that sense transcripts are produced, the segment being substantially homologous to the endogenous CHS gene and when transcribed in the transgenote being capable of effecting the flower color pattern modification; and selecting a plant exhibiting the modified flower color pattern.
Claim 5
Dependent Claim
10 words
A method of claim 2 wherein the promoter is constitutive.
Claim 6
Dependent Claim
15 words
A method of claim 2 wherein the promoter is a cauliflower mosaic virus 35S promoter.
Claim 7
Dependent Claim
10 words
A method of claim 2 wherein the promoter is heterologous.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-07-22 | 1995-01-21 | 1995-07-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-07-22 | 1999-01-21 | 1999-07-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-07-22 | 2003-01-21 | 2003-07-23 | $7700.00 | Window Closed / Lapsed |