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Patent US5013649A successfully refreshed from Google Patents.
US5013649A
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DNA sequences encoding osteoinductive products
Assignee: Genetics Institute LLC
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Filing: 1988-04-08
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Granted: 1991-05-07
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Expires: 2008-04-08
Abstract
Abstract Purified BMP-2 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Inventors
- 👤 Elizabeth A. Wang
- 👤 John M. Wozney
- 👤 Vicki Rosen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:31
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/51; A; A61; A61L; A61L24/00; A61L24/04; A61L24/043; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61K; A61K38/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
99 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleotide #1 through nucleotide #387 of figure I, (b) nucleotide #356 through nucleotide #1543 of figure II, (c) Nucleotide #402 through nucleotide #1626 of figure III, (d) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a), (b), and (c); and (e) sequences which (1) hybridize to any of sequences (a), (b), (c), or (d) under stringent hybridization conditions; and (2) encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 2
Dependent Claim
99 words
An isolated DNA sequence encoding an osteoinductive protein said DNA sequence comprising a coding sequence selected from the group consisting of (a) nucleotide #1 through nucleotide #387 of figure I, (b) nucleotide #356 through nucleotide #1543 of figure II, (c) Nucleotide #402 through nucleotide #1626 of figure III, (d) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a), (b), and (c); and (e) sequences which (1) hybridize to any of sequences (a), (b), (c), or (d) under stringent hybridization conditions; and (2) encode a protein characterized by the ability to induce the formation of bone and/or cartilage.
Claim 3
Dependent Claim
21 words
A vector comprising a DNA sequence of claim 1 in operative association with an expression control sequence for said DNA sequence.
Claim 4
Dependent Claim
25 words
A host cell transformed with the vector of claim 2 said host cells selected from the group consisting of mammalian, bacterial, yeast and insect cells.
Claim 5
Dependent Claim
39 words
A method for producing an osteoinductive protein, said method comprising the steps of (a) culturing in a suitable culture medium said transformed host cell of claim 3; and (b) isolating and purifying said osteoinductive protein from said culture medium.
Claim 6
Dependent Claim
14 words
The host cell of claim 4 wherein said host cell is a mammalian cell.
Claim 7
Dependent Claim
21 words
The host cell of claim 4 wherein said cells are selected from the group consisting of bacterial, yeast and insect cells.
Claim 8
Dependent Claim
11 words
A mammalian host cell transformed with the vector of claim 2.
References Cited (Backward Citations)
US4619989A
Cited
US4795804A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1994-05-06 | 1994-11-05 | 1995-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1998-05-06 | 1998-11-05 | 1999-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2002-05-06 | 2002-11-05 | 2003-05-07 | $7700.00 | Window Closed / Lapsed |