US4928603A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of preparing a cryoprecipitated suspension and use thereof
Assignee: Columbia University
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Filing: 1988-06-06
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Granted: 1990-05-29
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Expires: 2008-06-06
Abstract
Abstract This invention concerns a method of preparing a cryoprecipitated suspension containing fibrinogen and Factor XIII useful as a precursor in the preparation of a fibrin glue which involves (a) freezing fresh frozen plasma from a single donor such as a human or other animal, e.g., a cow, sheep or pig, which has been screened for blood transmitted diseases, e.g., one or more of syphilis, hepatitis or acquired immune deficiency syndrome at about -80° C. for at least 6 hours, preferably for at least about 12 hours; (b) raising the temperature, so as to form a supernatant and a cryoprecipitated suspension containing fibrinogen and Factor XIII; and (c) recovering the cryopricipitated suspension.
Inventors
- 👤 Eric Rose
- 👤 Arthur Dresdale
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 08:00
CPC Classifications
A; A61; A61L; A61L24/00; A61L24/04; A61L24/10; A61L24/106; A61L24/001; A61L24/0015; A61L2300/00; A61L2300/20; A61L2300/252; A61L2300/40; A61L2300/418
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
82 words
A method of preparing a cryoprecipitated suspension containing fibrinogen and Factor XIII useful as a precursor in the preparation of a fibrin glue which consists essentially of: (a) freezing fresh frozen plasma from a single donor which has been screened for blood transmitted diseases at about -80° C. for at least 6 hours, (b) raising the temperature of the frozen plasma so as to form a supernatant and a cryoprecipitated suspension containing fibrinogen and Factor XIII, and (c) recovering the cryoprecipitated suspension.
Claim 2
Independent Claim
82 words
A method of preparing a cryoprecipitated suspension containing fibrinogen and Factor XIII useful as a precursor in the preparation of a fibrin glue which consists essentially of: (a) freezing fresh frozen plasma from a single donor which has been screened for blood transmitted diseases at about -80° C. for at least 6 hours, (b) raising the temperature of the frozen plasma so as to form a supernatant and a cryoprecipitated suspension containing fibrinogen and Factor XIII, and (c) recovering the cryoprecipitated suspension.
Claim 3
Dependent Claim
14 words
A method of claim 1, wherein the donor is a human or other animal.
Claim 4
Dependent Claim
15 words
A method of claim 2, wherein the donor is a cow, sheep or a pig.
Claim 5
Dependent Claim
22 words
A method of claim 1, wherein the blood transmitted diseases comprise one or more of syphilis, hepatitis or acquired immune deficiency syndrome.
Claim 6
Dependent Claim
21 words
A method of claim 1, wherein the fresh frozen plasma is frozen in step (a) for at least about 12 hours.
Claim 7
Dependent Claim
26 words
A method of claim 1, wherein the temperature of the frozen plasma is raised in step (b) to between about 0° C. and about room temperature.
Claim 8
Dependent Claim
18 words
A method of claim 1, wherein recovering the cryoprecipitated suspension comprises decanting the supernatant from the cryoprecipitated suspension.
Claim 9
Dependent Claim
14 words
A method of claim 1 which further comprises concentrating the cryoprecipitated suspension by centrifugation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-05-28 | 1993-11-27 | 1994-05-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-05-28 | 1997-11-27 | 1998-05-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-05-28 | 2001-11-27 | 2002-05-29 | $7700.00 | Window Closed / Lapsed |