US4927676A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for rapid adherence of endothelial cells onto a surface and surfaces prepared thereby
Assignee: Becton Dickinson and Co
•
Filing: 1989-04-19
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Granted: 1990-05-22
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Expires: 2009-04-19
Abstract
Abstract Small diameter plastic tubing is plasma-treated on the lumen wall and endothelial cells are adhered thereto to give antithrombogenic small diameter conduits useful for vascular grafts and other articles intended to come in contact with blood.
Inventors
- 👤 Joel L. Williams
- 👤 David B. Montgomery
- 👤 Lillian P. Baldwin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-22 23:19
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/50; A61L27/507; A61L33/00; A61L33/0094; A61L33/18; Y; Y10; Y10T; Y10T428/00; Y10T428/13; Y10T428/1352; Y10T428/1386; Y10T428/29; Y10T428/2913; Y10T428/31504; Y10T428/31551; Y10T428/31786; Y10T428/31855; Y10T428/31938
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
61 words
A method for endothelialization of a polymeric surface comprising contacting a polymeric surface with a plasma generated from a gaseous material comprising nitrogen whereby said polymeric surface is modified to contain surface amino groups, and applying to said modified surface sufficient endothelial cells to form a confluent layer of cells on said amino group-containing surface without a requirement for cell proliferation.
Claim 2
Independent Claim
61 words
A method for endothelialization of a polymeric surface comprising contacting a polymeric surface with a plasma generated from a gaseous material comprising nitrogen whereby said polymeric surface is modified to contain surface amino groups, and applying to said modified surface sufficient endothelial cells to form a confluent layer of cells on said amino group-containing surface without a requirement for cell proliferation.
Claim 3
Dependent Claim
24 words
The method in accordance with claim 1 wherein said surface is selected from the group consisting of polyurethane, polystyrene, polyester, polyolefin and fluorinated polyolefin.
Claim 4
Dependent Claim
13 words
The method in accordance with claim 1 wherein said surface is relatively flat.
Claim 5
Dependent Claim
12 words
The method in accordance with claim 1 wherein said surface is curved.
Claim 6
Dependent Claim
34 words
The method in accordance with claim 1 wherein said material further comprises a second material selected from the group consisting of hydrogen, halogen, argon, neon, krypton and xenon, oxygen and a compound of oxygen.
Claim 7
Dependent Claim
68 words
The method in accordance with claim 1 wherein said plasma is generated using a measured power density level of about 0.001 to 400 watts per cubic centimeter generated over a frequency range from DC to 100 megahertz, said plasma being applied to said material for about 2 seconds to 12 hours at about 0° to 200° C., the pressure of said material being about 0.01 to 100 torr.
Claim 8
Dependent Claim
12 words
The method in accordance with claim 1 wherein said applying includes incubating.
Claim 9
Dependent Claim
28 words
The method in accordance with claim 7 wherein said incubating is carried out at a temperature of from about 10° to 50° C. for 1 to 120 minutes.
Claim 10
Independent Claim
60 words
A method for applying cells to a surface comprising contacting a surface with a plasma generated from a gas whereby said surface is modified to contain reactive functional groups from said gas and applying to said modified surface sufficient cells to form a confluent layer of cells on said surface containing said functional groups without a requirement for cell proliferation.
Claim 11
Independent Claim
60 words
A method for applying cells to a surface comprising contacting a surface with a plasma generated from a gas whereby said surface is modified to contain reactive functional groups from said gas and applying to said modified surface sufficient cells to form a confluent layer of cells on said surface containing said functional groups without a requirement for cell proliferation.
Claim 12
Dependent Claim
11 words
The method of claim 9 wherein said cells are epithelial cells.
Claim 13
Dependent Claim
24 words
The method in accordance with claim 9 wherein said surface is selected from the group consisting of an inorganic surface and a polymeric surface.
Claim 14
Dependent Claim
23 words
The method in accordance with claim 9 wherein said gas is selected from the group consisting of nitrogen, oxygen, and a compound thereof.
Claim 15
Independent Claim
66 words
A method comprising treating the lumen wall of a polymeric conduit with a plasma generated from a gas, said plasma causing bonding of a reactive functional group from said gas onto said lumen wall; and sodding said lumen wall containing said reactive functional group with sufficient endothelial cells to form a confluent layer of cells adhered to said lumen wall without a requirement for cell proliferation.
Claim 16
Independent Claim
66 words
A method comprising treating the lumen wall of a polymeric conduit with a plasma generated from a gas, said plasma causing bonding of a reactive functional group from said gas onto said lumen wall; and sodding said lumen wall containing said reactive functional group with sufficient endothelial cells to form a confluent layer of cells adhered to said lumen wall without a requirement for cell proliferation.
Claim 17
Dependent Claim
21 words
The method of claim 13 wherein the lumen wall to be treated has an inside diameter of 4 mm or less.
Claim 18
Dependent Claim
23 words
The method in accordance with claim 13 wherein said gas is selected from the group consisting of oxygen, nitrogen and a compound thereof.
Claim 19
Dependent Claim
27 words
An antithrombogenic article comprising a plasma-treated polymeric surface having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said surface having amino groups.
Claim 20
Dependent Claim
27 words
An antithrombogenic article comprising a plasma-treated polymeric surface having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said surface having amino groups.
Claim 21
Dependent Claim
35 words
The article in accordance with claim 16 wherein said polymeric surface is selected from the group consisting of a polyolefin surface, a polyurethane surface, a fluorinated polyolefin surface, a polyester surface and a polystyrene surface.
Claim 22
Dependent Claim
23 words
The article in accordance with claim 16 selected from the group consisting of a plate, strip, film, sheet, fiber, fabric, conduit and cast.
Claim 23
Dependent Claim
28 words
An antithrombogenic article comprising a plasma-treated surface having reactive functional groups bonded thereto and a confluent layer of epithelial cells adhered to said surface containing reactive functional groups.
Claim 24
Dependent Claim
28 words
An antithrombogenic article comprising a plasma-treated surface having reactive functional groups bonded thereto and a confluent layer of epithelial cells adhered to said surface containing reactive functional groups.
Claim 25
Dependent Claim
24 words
The article in accordance with claim 19 wherein said functional groups are selected from the group consisting of amino groups and groups containing oxygen.
Claim 26
Dependent Claim
41 words
A polymeric conduit having an inside diameter less than about 6 mm comprising a plasma-treated lumen wall, said wall having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said lumen wall whereby said conduit is antithrombogenic.
Claim 27
Dependent Claim
41 words
A polymeric conduit having an inside diameter less than about 6 mm comprising a plasma-treated lumen wall, said wall having amino groups bonded thereto and a confluent layer of endothelial cells adhered to said lumen wall whereby said conduit is antithrombogenic.
References Cited (Backward Citations)
EP0206025A2
Cited
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