Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
35 words
An implantable prosthetic surface for implantation in a human patient, comprising: a synthetic substrate and a surface layer, said surface layer comprising Type IV/V collagen and/or human endothelial cells present at least about 50% confluence.
Claim 2
Dependent Claim
35 words
An implantable prosthetic surface for implantation in a human patient, comprising: a synthetic substrate and a surface layer, said surface layer comprising Type IV/V collagen and/or human endothelial cells present at least about 50% confluence.
Claim 3
Dependent Claim
12 words
The prosthetic surface of claim 1, further comprising an intermediate protein layer.
Claim 4
Dependent Claim
25 words
The prosthetic surface of claim 2, wherein said protein layer is an interstitial collagen layer disposed between said substrate and said Type IV/V collagen layer.
Claim 5
Dependent Claim
17 words
The prosthetic surface of claim 1, comprising a linking agent linking said substrate to said surface layer.
Claim 6
Dependent Claim
27 words
The prosthetic surface of claim 4, wherein said linking agent is gluteraldehyde and wherein the free aldehyde groups of said gluteraldehyde have been substantially deactivated following linkage.
Claim 7
Dependent Claim
21 words
The prosthetic surface of claim 3, wherein said interstitial collagen layer and said Type IV/V collagen layer are human chorioallantoic membrane.
Claim 8
Dependent Claim
18 words
The prosthetic surface of any of the preceding claims, wherein said endothelial cells are uncultured, autologous endothelial cells.
Claim 9
Dependent Claim
18 words
The prosthetic surface of any of the preceding claims, wherein said endothelial cells are confluent prior to implantation.
Claim 10
Dependent Claim
23 words
The prosthetic surface of any of the preceding claims, wherein said endothelial cells are autologous endothelial cells exhibiting a substantially flat, cobblestone morphology.
Claim 11
Dependent Claim
18 words
The prosthetic surface of any of the preceding claims, wherein said endothelial cells are fat derived endothelial cells.
Claim 12
Dependent Claim
17 words
The prosthetic surface of any of the preceding claims, wherein said endothelial cells are microvascular endothelial cells.
Claim 13
Dependent Claim
31 words
The prosthetic surface of any of the preceding claims, wherein said prosthetic surface is the surface of a vascular graft having an internal diameter of no more than about 4 mm.
Claim 14
Independent Claim
63 words
A method of treating an implant intended for implantation in a human patient, comprising: (a) providing a synthetic substrate material; (b) binding an interstitial collagen layer to said substrate material; (c) binding a Type IV/V collagen surface layer to said interstitial collagen layer; and (d) adhering human endothelial cells to said Type IV/V collagen surface layer at least 50% confluence prior to implantation.
Claim 15
Independent Claim
63 words
A method of treating an implant intended for implantation in a human patient, comprising: (a) providing a synthetic substrate material; (b) binding an interstitial collagen layer to said substrate material; (c) binding a Type IV/V collagen surface layer to said interstitial collagen layer; and (d) adhering human endothelial cells to said Type IV/V collagen surface layer at least 50% confluence prior to implantation.
Claim 16
Dependent Claim
24 words
The method of claim 13, wherein said collagen layers are bound using a linking agent which is deactivated after binding and prior to implantation.
Claim 17
Dependent Claim
29 words
The method of claim 13, wherein said Type IV/V collagen surface layer is bound by binding the interstitial collagen surface of human chorioallantoic membrane to said interstitial collagen layer.