Patent US4902295A successfully refreshed from Google Patents.
US4902295A
Expired / Lapsed
US
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Transplantable artificial tissue
Assignee: Somatix Therapy Corp
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Filing: 1987-11-06
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Granted: 1990-02-20
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Expires: 2007-11-06
Abstract
Abstract A transplantable artificial tissue matrix structure containing viable cells which is suitable for insertion into the body is made by polymerizing precursors in an aqueous solution to form a shape retaining solid matrix comprising viable cells, matrix polymer and reversible gel polymer. The solution contains a matrix polymer precursor, a reversible gel polymer precursor, and viable cells. The reversible gel polymer is dissolved and removed to yield an insoluble, porous matrix containing viable cells. The conditions and reagents are selected to maintain the viability of the cells. The invention is particularly suitable for artificial transplant matrix tissue containing pancreatic islet cells.
Inventors
- 👤 Bennie J. Walthall
- 👤 Yvonne E. McHugh
- 👤 Houston F. Voss
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:38
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/14; A61K9/16; A61K9/1682; A61K9/1694; A61K35/00; A61K35/12; A61K35/37; A61K35/39; A61K9/1605; A61K9/1629; A61K9/1652; A61K9/1658; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/50; A61L27/60
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
92 words
A matrix composition comprising viable cells incorporated throughout a porous matrix of non-toxic biocompatible polymer substantially free from fibroblasts, said biocompatible polymer consisting essentially of the product of the polymerizations of a matrix polymer precursor and a reversible gel polymer, the matrix having a particle size of from 0.02 to 3 mm; a pore structure and size permitting cell movement throughout the matrix, movement of host cells into the matrix, diffusion of nutrients and macromolecules into and out of the matrix; wherein the composition is free from encapsulation in a semi-permeable membrane.
Claim 2
Dependent Claim
92 words
A matrix composition comprising viable cells incorporated throughout a porous matrix of non-toxic biocompatible polymer substantially free from fibroblasts, said biocompatible polymer consisting essentially of the product of the polymerizations of a matrix polymer precursor and a reversible gel polymer, the matrix having a particle size of from 0.02 to 3 mm; a pore structure and size permitting cell movement throughout the matrix, movement of host cells into the matrix, diffusion of nutrients and macromolecules into and out of the matrix; wherein the composition is free from encapsulation in a semi-permeable membrane.
Claim 3
Dependent Claim
39 words
The matrix composition of claim 1 wherein the matrix is a product of the polymerization of a matrix polymer precursor selected from the group consisting of plasma, fibrinogen, casein, fibrin, limulus lysate, milk protein and collagen and mixtures thereof.
Claim 4
Dependent Claim
14 words
The matrix composition of claim 1 wherein the viable cells are pancreatic islet cells.
Claim 5
Dependent Claim
85 words
The matrix composition of claim 1 produced by (a) polymerizing polymer precursors in an aqueous solution containing a matrix polymer precursor, a reversible gel polymer precursor, and viable cells to form a shape retaining solid matrix comprising viable cells, matrix polymer and reversible gel polymer, (b) dissolving gel polymer and removing gel polymer dissolution products from the matrix, and (c) recovering an insoluble, porous, unencapsulated matrix containing viable cells, wherein the conditions and reagents are selected to not significantly impair the viability of the cells.
Claim 6
Dependent Claim
14 words
The matrix composition of claim 4 wherein the viable cells are pancreatic islet cells.
Claim 7
Dependent Claim
142 words
The matrix composition of claim 1 produced by (a) polymerizing polymers precursors in an aqueous solution containing a matrix polymer precursor, a reversible gel polymer precursor, and viable cells to form a shape retaining solid matrix comprising viable cells, matrix polymer and reversible gel polymer, (i) wherein the matrix polymer precursor is selected from the group consisting of plasma, fibrinogen, casein, fibrin, limulus lysate, milk protein, collagen, and mixtures thereof, and (ii) the reversible gel polymer precursor is a member selected from the group consisting of agarose, carrageenan, agar, alginate, guar gum, gum arabic, pectin, tragacanth gum, xanthan gum, and mixtures thereof, (b) dissolving gel polymer and removing gel polymer dissolution products from the matrix, and (c) recovering an insoluble, porous, unencapsulated matrix containing viable cells, wherein the conditions and reagents are selected to not significantly impair the viability of the cells.
Claim 8
Dependent Claim
21 words
The matrix composition of claim 1 wherein the aqueous solution from which the matrix composition is formed contained fibronectin and laminin.
References Cited (Backward Citations)
US4645669A
Cited
US4722898A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1993-02-19 | 1993-08-21 | 1994-02-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-02-19 | 1997-08-21 | 1998-02-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-02-19 | 2001-08-21 | 2002-02-20 | $7700.00 | Window Closed / Lapsed |