US4895802A
Expired / Lapsed
US
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DNA strand coding for α-acetolactate decarboxylase and yeast transformed with the DNA strand
Assignee: Kirin Brewery Co Ltd
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Filing: 1986-12-11
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Granted: 1990-01-23
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Expires: 2006-12-11
Abstract
Abstract DNA strand having an ability in biotechnological production of α-acetolactate decarboxylase is disclosed. The DNA strand is characterized in that it has a nucleotide sequence coding for a polypeptide whose amino acid sequence is substantially from A to B of FIG. 1 and which has α-acetolactate decarboxcylase activity. Also disclosed is a yeast which belongs to Saccharomyces cerevisiae and which has been transformed by the DNA strand. The yeast is characterized by the face that its α-acetolactate producing ability is reduced, and will thus produce an alcoholic liquor such as beer which contains no or little diacetyls which have come from their precursor, namely α-acetolactate.
Inventors
- 👤 Hidetaka Sone
- 👤 Junichi Tanaka
- 👤 Takashi Inoue
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:00
CPC Classifications
C; C12; C12C; C12C11/00; C12C11/02; C12C11/003; C12C12/00; C12C12/002; C12C12/006; C12C5/00; C12C5/004; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12N15/87; C12N15/90; C12N9/00; C12N9/88; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/94
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
37 words
An isolated DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 2
Dependent Claim
37 words
An isolated DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 3
Dependent Claim
29 words
An isolated DNA sequence of claim 1, which has a nucleotide sequence of from A to B of the nucleotide sequence shown in FIG. 1 or its degenerative isomer.
Claim 4
Dependent Claim
52 words
A yeast belonging to Saccharomyces cerevisiae, having reduced α-acetolactate producing ability which has been transformed with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 5
Dependent Claim
52 words
A yeast belonging to Saccharomyces cerevisiae, having reduced α-acetolactate producing ability which has been transformed with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 6
Dependent Claim
15 words
A yeast according to claim 3, wherein the nucleotide sequence is contained in a plasmid.
Claim 7
Dependent Claim
27 words
A yeast according to claim 3, wherein the DNA sequence has the nucleotide sequence of from A to B of the nucleotide sequence shown in FIG. 1.
Claim 8
Dependent Claim
59 words
A process for producing a yeast having reduced α-acetolactate producing ability, which process comprises transforming a host yeast belonging to Saccharomyces cerevisiae with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 9
Dependent Claim
59 words
A process for producing a yeast having reduced α-acetolactate producing ability, which process comprises transforming a host yeast belonging to Saccharomyces cerevisiae with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 10
Dependent Claim
38 words
A process for producing a yeast having reduced α-acetolactate producing ability as claimed in claim 6, wherein the nucleotide sequence is one from A to B of the nucleotide sequence shown in FIG. 1 or its degenerative isomer.
Claim 11
Dependent Claim
25 words
A process for producing a yeast having reduced α-acetolactate producing ability as claimed in claim 6, wherein the nucleotide sequence is contained in a plasmid.
Claim 12
Dependent Claim
37 words
A process for producing a yeast having reduced α-acetolactate producing ability as claimed in claim 7, wherein the DNA sequence has the nucleotide sequence of from A to B of the nucleotide sequence shown in FIG. 1.
Claim 13
Dependent Claim
81 words
A process for producing an alcoholic beverage wherein a yeast is caused to act upon its substrate to produce ethanol therefrom, the improvement which comprises the use of a yeast belonging to Saccharomyces cerevisiae having reduced α-acetolactate producing ability, which yeast has been transformed with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 14
Dependent Claim
81 words
A process for producing an alcoholic beverage wherein a yeast is caused to act upon its substrate to produce ethanol therefrom, the improvement which comprises the use of a yeast belonging to Saccharomyces cerevisiae having reduced α-acetolactate producing ability, which yeast has been transformed with a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 15
Dependent Claim
21 words
The process for producing an alcoholic beverage as claimed in claim 10, wherein the nucleotide sequence is contained in a plasmid.
Claim 16
Dependent Claim
33 words
The process for producing an alcoholic beverage as claimed in claim 10, wherein the DNA sequence has a nucleotide sequence of from A to B of the nucleotide sequence shown in FIG. 1.
Claim 17
Dependent Claim
18 words
The process for producing an alcoholic beverage as claimed in claim 10, wherein the alcoholic beverage is beer.
Claim 18
Dependent Claim
42 words
A recombinant DNA vector having a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which the amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
Claim 19
Dependent Claim
42 words
A recombinant DNA vector having a DNA sequence which has a nucleotide sequence coding for a polypeptide having α-acetolactate decarboxylase activity, of which the amino acid sequence is substantially from A to B of the amino acid sequence shown in FIG. 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1993-01-22 | 1993-07-24 | 1994-01-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1997-01-22 | 1997-07-24 | 1998-01-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2001-01-22 | 2001-07-24 | 2002-01-23 | $7700.00 | Window Closed / Lapsed |