EP0128714A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Alpha-acetolactate decarboxylase enzyme and preparation thereof
Assignee: Novo Nordisk AS
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Filing: 1984-06-01
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Granted: —
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Expires: 2004-06-01
Abstract
Abstract A novel a-acetolactate decarboxylase enzyme product with improved stability is provided. The novel enzyme can be produced in high levels by cultivation of Bacillus strains selected from the group consisting of Bacillus brevis and Bacillus licheniformis.
Inventors
- 👤 Frank Olsen
- 👤 Knud Aunstrup
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 16:23
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/88; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/07; C12R2001/08; C12R2001/10; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/833; Y10S435/836
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
43 words
An a-acetolactate decarboxylase enzyme obtainable by cultivation in a suitable nutrient medium of Bacillus brevis strain ATCC 11031 or Bacillus licheniformis strains ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537 and, NCIB 11868.
Claim 2
Dependent Claim
43 words
An a-acetolactate decarboxylase enzyme obtainable by cultivation in a suitable nutrient medium of Bacillus brevis strain ATCC 11031 or Bacillus licheniformis strains ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537 and, NCIB 11868.
Claim 3
Dependent Claim
25 words
An a-acetolactate decarboxylase according to claim 1 wherein the a-acetolactate decarboxylase activity is in the range of from 0.1 to 10 NU per mg protein.
Claim 4
Dependent Claim
64 words
A process for the preparation of an a-acetolactate decarboxylase enzyme according to claim 1, which process comprises cultivation in a suitable nutrient medium containing carbon and nitrogen sources and inorganic salts of an a-acetolactate decarboxylase producing Bacillus strain selected from the group consisting of Bacillus brevis and Bacillus licheniformis strains and thereafter recovery of the a-acetolactate decarboxylase enzyme from the cells in the medium.
Claim 5
Dependent Claim
27 words
A process according to claim 3 in which the Bacillus brevis strain is ATCC 11031 or a mutant or variant thereof productive of the a-acetolactate decarboxylase enzyme.
Claim 6
Dependent Claim
51 words
A process according to claim 3 in which the Bacillus licheniformis strain is selected from the group consisting of ATCC 12759, ATCC 12713, ATCC 11946, ATCC 27326, NRRL B-3751, NCTC 2120, NCTC 8721, NCIB 6816, NCIB 8537, NCIB 11868 , and mutants and variations thereof productive of the a-acetolactate decarboxylase enzyme.
Claim 7
Dependent Claim
29 words
A process according to claim 5 wherein the nutrient medium comprises a nitrogen source whose content of free amino acids does not repress the formation of the a-acetolactate decarboxylase.
Claim 8
Dependent Claim
15 words
A process according to claim 6 wherein the nitrogen source is an inorganic nitrogen source.
References Cited (Backward Citations)
No citation records indexed.
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