Patent US4885172A successfully refreshed from Google Patents.
US4885172A
Expired / Lapsed
US
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Composition for targeting, storing and loading of liposomes
Assignee: LIPSOME COMPANY Inc
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Filing: 1986-12-15
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Granted: 1989-12-05
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Expires: 2006-12-15
Abstract
Abstract The present invention describes a composition consisting of liposomes covalently or non-covalently coupled to the glycoprotein streptavidin. The streptavidin may additionally be coupled to biotinated proteins such as Immunoglobulin G or monoclonal antibodies. The liposomes of the invention may have a transmembrane potential across their membranes, and may be dehydrated. In addition, the composition may contain ionizable bioactive agents such as antineoplastic agents, and may be used in diagnostic assays.
Inventors
- 👤 Marcel B. Bally
- 👤 Helen Loughrey
- 👤 Pieter R. Cullis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:25
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K47/00; A61K47/50; A61K47/51; A61K47/62; A61K47/69; A61K47/6905; A61K47/6911; A61K47/6913
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
3 words
A streptavidin-coupled liposome.
Claim 2
Dependent Claim
3 words
A streptavidin-coupled liposome.
Claim 3
Dependent Claim
15 words
The streptavidin-coupled liposome of claim 1 wherein the streptavidin is coupled to the liposome covalently.
Claim 4
Dependent Claim
15 words
The streptavidin-coupled liposome of claim 1 wherein the streptavidin is coupled to the liposome non-covalently.
Claim 5
Dependent Claim
13 words
The streptavidin-coupled liposome of claim 1 wherein the liposome contains a bioactive agent.
Claim 6
Dependent Claim
13 words
The streptavidin-coupled liposome of claim 1 wherein the liposome has a transmembrane potential.
Claim 7
Dependent Claim
13 words
The streptavidin-coupled liposome of claim 5 wherein the liposome contains a bioactive agent.
Claim 8
Dependent Claim
11 words
The streptavidin-coupled liposome of claim 1 wherein the composition is dehydrated.
Claim 9
Dependent Claim
24 words
The streptavidin-coupled liposome of claim 1 wherein the liposome contains a bioactive agent and has a transmembrane potential, and wherein the composition is dehydrated.
Claim 10
Dependent Claim
17 words
A pharmaceutical composition comprising the streptavidin-coupled liposome of claim 1 and a pharmaceutically acceptable carrier or diluent.
Claim 11
Dependent Claim
15 words
The streptavidin-coupled liposome of claim 1 wherein streptavidin is additionally coupled to a biotinated protein.
Claim 12
Dependent Claim
12 words
The streptavidin-coupled liposome of claim 10 wherein the protein is Immunoglobulin G.
Claim 13
Dependent Claim
13 words
The streptavidin-coupled liposome of claim 10 wherein the protein is a monoclonal antibody.
Claim 14
Independent Claim
27 words
A method for preparing a liposome non-covalently coupled to streptavidin comprising the steps of: (a) preparing liposomes comprising biotinylated phosphatidylethanolamine; and (b) incubating the liposomes in streptavidin.
Claim 15
Independent Claim
27 words
A method for preparing a liposome non-covalently coupled to streptavidin comprising the steps of: (a) preparing liposomes comprising biotinylated phosphatidylethanolamine; and (b) incubating the liposomes in streptavidin.
Claim 16
Dependent Claim
12 words
The method of claim 13 wherein the liposomes are large unilamellar vesicles.
Claim 17
Dependent Claim
12 words
The method of claim 13 wherein the liposomes also comprise egg phosphatidylcholine.
Claim 18
Dependent Claim
20 words
The method of claim 15 wherein the biotinylated phosphatidylethanolamine is in an about 0.1%-0.5% mole ratio with the egg phosphatidylcholine.
Claim 19
Dependent Claim
20 words
The method of claim 16 wherein the biotinylated phosphatidylethanolamine is in an about 0.1% mole ratio with the egg phosphatidylcholine.
Claim 20
Dependent Claim
21 words
The method of claim 13 wherein step (b) is performed in about 10 fold molar excess of streptavidin to biotinylated phosphatidylethanolamine.
Claim 21
Dependent Claim
41 words
A method for preparing a protein-coupled streptavidin-biotinylated phosphatidylethanolamine-containing liposome wherein streptavidin is non-covalently coupled to the liposome, comprising the steps of: (a) forming the streptavidin-coupled biotinylated phosphatidylethanolamine liposome of claim 13, and (b) incubating a biotinylated fluorescent-amine-labelled protein with the liposomes.
Claim 22
Dependent Claim
18 words
A composition comprising a protein coupled to streptavidin which is non-covalently coupled to a liposome comprising biotinylated phosphatidylethanolamine.
Claim 23
Dependent Claim
18 words
A composition comprising a protein coupled to streptavidin which is non-covalently coupled to a liposome comprising biotinylated phosphatidylethanolamine.
Claim 24
Dependent Claim
13 words
The composition of claim 20 wherein the liposome is a large unilamellar vesicle.
Claim 25
Dependent Claim
15 words
The composition of claim 20 wherein the protein is an immunoglobulin or a monoclonal antibody.
Claim 26
Dependent Claim
11 words
The composition of claim 22 wherein the immunoglobulin is Immunoglobulin G.
Claim 27
Dependent Claim
10 words
The composition of claim 20 additionally comprising a bioactive agent.
Claim 28
Dependent Claim
16 words
A pharmaceutical composition comprising the composition of claim 20 and a suitable pharmaceutical carrier or diluent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-12-04 | 1993-06-05 | 1993-12-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-04 | 1997-06-05 | 1997-12-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-04 | 2001-06-05 | 2001-12-05 | $7700.00 | Window Closed / Lapsed |